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Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2
OBJECTIVE: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the pathogen causing the coronavirus disease 2019 (COVID-19) global pandemic. Recent studies have shown the importance of the throat and salivary glands as sites of virus replication and transmission. The viral host receptor,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
sian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI. Published by Elsevier Ltd.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010230/ https://www.ncbi.nlm.nih.gov/pubmed/35441076 http://dx.doi.org/10.1016/j.ajoms.2022.04.001 |
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author | Okamoto, Nako Saito, Akatsuki Okabayashi, Tamaki Komine, Akihiko |
author_facet | Okamoto, Nako Saito, Akatsuki Okabayashi, Tamaki Komine, Akihiko |
author_sort | Okamoto, Nako |
collection | PubMed |
description | OBJECTIVE: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the pathogen causing the coronavirus disease 2019 (COVID-19) global pandemic. Recent studies have shown the importance of the throat and salivary glands as sites of virus replication and transmission. The viral host receptor, angiotensin-converting enzyme 2 (ACE2), is broadly enriched in epithelial cells of the salivary glands and oral mucosae. Oral care products containing cetylpyridinium chloride (CPC) as a bactericidal ingredient are known to exhibit antiviral activity against SARS-CoV-2 in vitro. However, the exact mechanism of action remains unknown. METHODS: This study examined the antiviral activity of CPC against SARS-CoV-2 and its inhibitory effect on the interaction between the viral spike (S) protein and ACE2 using an enzyme-linked immunosorbent assay. RESULTS: CPC (0.05%, 0.1% and 0.3%) effectively inactivated SARS-CoV-2 within the contact times (20 and 60 s) in directions for use of oral care products in vitro. The binding ability of both the S protein and ACE2 were reduced by CPC. CONCLUSIONS: Our results suggest that CPC inhibits the interaction between S protein and ACE2, and thus, reduces infectivity of SARS-CoV-2 and suppresses viral adsorption. |
format | Online Article Text |
id | pubmed-9010230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | sian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI. Published by Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90102302022-04-15 Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 Okamoto, Nako Saito, Akatsuki Okabayashi, Tamaki Komine, Akihiko J Oral Maxillofac Surg Med Pathol Original Research OBJECTIVE: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the pathogen causing the coronavirus disease 2019 (COVID-19) global pandemic. Recent studies have shown the importance of the throat and salivary glands as sites of virus replication and transmission. The viral host receptor, angiotensin-converting enzyme 2 (ACE2), is broadly enriched in epithelial cells of the salivary glands and oral mucosae. Oral care products containing cetylpyridinium chloride (CPC) as a bactericidal ingredient are known to exhibit antiviral activity against SARS-CoV-2 in vitro. However, the exact mechanism of action remains unknown. METHODS: This study examined the antiviral activity of CPC against SARS-CoV-2 and its inhibitory effect on the interaction between the viral spike (S) protein and ACE2 using an enzyme-linked immunosorbent assay. RESULTS: CPC (0.05%, 0.1% and 0.3%) effectively inactivated SARS-CoV-2 within the contact times (20 and 60 s) in directions for use of oral care products in vitro. The binding ability of both the S protein and ACE2 were reduced by CPC. CONCLUSIONS: Our results suggest that CPC inhibits the interaction between S protein and ACE2, and thus, reduces infectivity of SARS-CoV-2 and suppresses viral adsorption. sian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI. Published by Elsevier Ltd. 2022-11 2022-04-15 /pmc/articles/PMC9010230/ /pubmed/35441076 http://dx.doi.org/10.1016/j.ajoms.2022.04.001 Text en © 2022Asian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Original Research Okamoto, Nako Saito, Akatsuki Okabayashi, Tamaki Komine, Akihiko Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 |
title | Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 |
title_full | Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 |
title_fullStr | Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 |
title_full_unstemmed | Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 |
title_short | Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 |
title_sort | virucidal activity and mechanism of action of cetylpyridinium chloride against sars-cov-2 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010230/ https://www.ncbi.nlm.nih.gov/pubmed/35441076 http://dx.doi.org/10.1016/j.ajoms.2022.04.001 |
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