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Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst
Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (Cu(x)O/TiO(2)) inactivated the severe acute respiratory syndrome corona...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010443/ https://www.ncbi.nlm.nih.gov/pubmed/35422456 http://dx.doi.org/10.1038/s41598-022-09402-7 |
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author | Nakano, Ryuichi Yamaguchi, Akira Sunada, Kayano Nagai, Takeshi Nakano, Akiyo Suzuki, Yuki Yano, Hisakazu Ishiguro, Hitoshi Miyauchi, Masahiro |
author_facet | Nakano, Ryuichi Yamaguchi, Akira Sunada, Kayano Nagai, Takeshi Nakano, Akiyo Suzuki, Yuki Yano, Hisakazu Ishiguro, Hitoshi Miyauchi, Masahiro |
author_sort | Nakano, Ryuichi |
collection | PubMed |
description | Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (Cu(x)O/TiO(2)) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the Cu(x)O/TiO(2) photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the Cu(x)O/TiO(2) photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The Cu(x)O/TiO(2) photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night. |
format | Online Article Text |
id | pubmed-9010443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90104432022-04-18 Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst Nakano, Ryuichi Yamaguchi, Akira Sunada, Kayano Nagai, Takeshi Nakano, Akiyo Suzuki, Yuki Yano, Hisakazu Ishiguro, Hitoshi Miyauchi, Masahiro Sci Rep Article Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (Cu(x)O/TiO(2)) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the Cu(x)O/TiO(2) photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the Cu(x)O/TiO(2) photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The Cu(x)O/TiO(2) photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night. Nature Publishing Group UK 2022-04-14 /pmc/articles/PMC9010443/ /pubmed/35422456 http://dx.doi.org/10.1038/s41598-022-09402-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nakano, Ryuichi Yamaguchi, Akira Sunada, Kayano Nagai, Takeshi Nakano, Akiyo Suzuki, Yuki Yano, Hisakazu Ishiguro, Hitoshi Miyauchi, Masahiro Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst |
title | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst |
title_full | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst |
title_fullStr | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst |
title_full_unstemmed | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst |
title_short | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO(2)-based photocatalyst |
title_sort | inactivation of various variant types of sars-cov-2 by indoor-light-sensitive tio(2)-based photocatalyst |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010443/ https://www.ncbi.nlm.nih.gov/pubmed/35422456 http://dx.doi.org/10.1038/s41598-022-09402-7 |
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