Cargando…

Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion

Probiotics are known to contribute to the anti-oxidation, immunoregulation, and aging delay. Here, we investigated the extension of lifespan by fermented pickles-origin Pediococcus acidilactici (PA) in Caenorhabditis elegans (C. elegans), and found that PA promoted a significantly extended longevity...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Rui, Zhang, Yong, Qian, Weiyi, Leng, Yan, Long, Yan, Liu, Xinjie, Li, Jinping, Wan, Xiangyuan, Wei, Xun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010657/
https://www.ncbi.nlm.nih.gov/pubmed/35433778
http://dx.doi.org/10.3389/fnut.2022.821685
_version_ 1784687527576207360
author Hu, Rui
Zhang, Yong
Qian, Weiyi
Leng, Yan
Long, Yan
Liu, Xinjie
Li, Jinping
Wan, Xiangyuan
Wei, Xun
author_facet Hu, Rui
Zhang, Yong
Qian, Weiyi
Leng, Yan
Long, Yan
Liu, Xinjie
Li, Jinping
Wan, Xiangyuan
Wei, Xun
author_sort Hu, Rui
collection PubMed
description Probiotics are known to contribute to the anti-oxidation, immunoregulation, and aging delay. Here, we investigated the extension of lifespan by fermented pickles-origin Pediococcus acidilactici (PA) in Caenorhabditis elegans (C. elegans), and found that PA promoted a significantly extended longevity of wild-type C. elegans. The further results revealed that PA regulated the longevity via promoting the insulin/IGF-1 signaling, JNK/MAPK signaling but not TOR signaling in C. elegans, and that PA reduced the reactive oxygen species (ROS) levels and modulated expression of genes involved in fatty acids uptake and lipolysis, thus reducing the fat accumulation in C. elegans. Moreover, this study identified the nrfl-1 as the key regulator of the PA-mediated longevity, and the nrfl-1/daf-18 signaling might be activated. Further, we highlighted the roles of one chloride ion exchanger gene sulp-6 in the survival of C. elegans and other two chloride ion channel genes clh-1 and clh-4 in the prolonged lifespan by PA-feeding through the modulating expression of genes involved in inflammation. Therefore, these findings reveal the detailed and novel molecular mechanisms on the longevity of C. elegans promoted by PA.
format Online
Article
Text
id pubmed-9010657
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90106572022-04-16 Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion Hu, Rui Zhang, Yong Qian, Weiyi Leng, Yan Long, Yan Liu, Xinjie Li, Jinping Wan, Xiangyuan Wei, Xun Front Nutr Nutrition Probiotics are known to contribute to the anti-oxidation, immunoregulation, and aging delay. Here, we investigated the extension of lifespan by fermented pickles-origin Pediococcus acidilactici (PA) in Caenorhabditis elegans (C. elegans), and found that PA promoted a significantly extended longevity of wild-type C. elegans. The further results revealed that PA regulated the longevity via promoting the insulin/IGF-1 signaling, JNK/MAPK signaling but not TOR signaling in C. elegans, and that PA reduced the reactive oxygen species (ROS) levels and modulated expression of genes involved in fatty acids uptake and lipolysis, thus reducing the fat accumulation in C. elegans. Moreover, this study identified the nrfl-1 as the key regulator of the PA-mediated longevity, and the nrfl-1/daf-18 signaling might be activated. Further, we highlighted the roles of one chloride ion exchanger gene sulp-6 in the survival of C. elegans and other two chloride ion channel genes clh-1 and clh-4 in the prolonged lifespan by PA-feeding through the modulating expression of genes involved in inflammation. Therefore, these findings reveal the detailed and novel molecular mechanisms on the longevity of C. elegans promoted by PA. Frontiers Media S.A. 2022-04-01 /pmc/articles/PMC9010657/ /pubmed/35433778 http://dx.doi.org/10.3389/fnut.2022.821685 Text en Copyright © 2022 Hu, Zhang, Qian, Leng, Long, Liu, Li, Wan and Wei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Hu, Rui
Zhang, Yong
Qian, Weiyi
Leng, Yan
Long, Yan
Liu, Xinjie
Li, Jinping
Wan, Xiangyuan
Wei, Xun
Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion
title Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion
title_full Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion
title_fullStr Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion
title_full_unstemmed Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion
title_short Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion
title_sort pediococcus acidilactici promotes the longevity of c. elegans by regulating the insulin/igf-1 and jnk/mapk signaling, fat accumulation and chloride ion
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010657/
https://www.ncbi.nlm.nih.gov/pubmed/35433778
http://dx.doi.org/10.3389/fnut.2022.821685
work_keys_str_mv AT hurui pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT zhangyong pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT qianweiyi pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT lengyan pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT longyan pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT liuxinjie pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT lijinping pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT wanxiangyuan pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion
AT weixun pediococcusacidilacticipromotesthelongevityofcelegansbyregulatingtheinsulinigf1andjnkmapksignalingfataccumulationandchlorideion