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Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway

Premature ovarian insufficiency (POI) poses a great threat to reproductive-age women. Ovarian fibrogenesis is a basic histologic feature of POI. Ovarian theca–stroma cells are responsible for ovarian fibrosis, but few studies have focused on the ovarian microenvironment. The role and mechanism of ch...

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Autores principales: Wang, Chaojun, Sun, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010671/
https://www.ncbi.nlm.nih.gov/pubmed/35432206
http://dx.doi.org/10.3389/fendo.2022.823740
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author Wang, Chaojun
Sun, Yun
author_facet Wang, Chaojun
Sun, Yun
author_sort Wang, Chaojun
collection PubMed
description Premature ovarian insufficiency (POI) poses a great threat to reproductive-age women. Ovarian fibrogenesis is a basic histologic feature of POI. Ovarian theca–stroma cells are responsible for ovarian fibrosis, but few studies have focused on the ovarian microenvironment. The role and mechanism of chemokines in the development of POI remain unclear. Here, we evaluated C-X-C motif chemokine ligand 10 (CXCL10) in biochemical POI patients, POI patients, and a POI mouse model. CXCL10 levels in serum and follicular fluid were higher in both bPOI and POI patients than in controls. An increased level of CXCL10 was also observed in a POI mouse model. CXCL10 concentrations in serum and follicular fluid were positively associated with follicle-stimulating hormone and negatively associated with antral follicle count. Our study for the first time found that CXCL10 induced COL1A1 and COL1A2 production, two subunits of collagen I in mouse theca–stroma cells by activating the JNK/c-Jun pathway. Inhibition of JNK and c-Jun attenuated the increases of COL1A1 and COL1A2 caused by CXCL10. Moreover, CXCL10 had no effects on hormone synthesis, proliferation, and apoptosis in human luteinized granulosa (hGL) cells. Our findings revealed a potential diagnostic value of CXCL10 in the early stage of POI and shed new insights into the biological function of CXCL10 in ovarian fibrosis.
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spelling pubmed-90106712022-04-16 Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway Wang, Chaojun Sun, Yun Front Endocrinol (Lausanne) Endocrinology Premature ovarian insufficiency (POI) poses a great threat to reproductive-age women. Ovarian fibrogenesis is a basic histologic feature of POI. Ovarian theca–stroma cells are responsible for ovarian fibrosis, but few studies have focused on the ovarian microenvironment. The role and mechanism of chemokines in the development of POI remain unclear. Here, we evaluated C-X-C motif chemokine ligand 10 (CXCL10) in biochemical POI patients, POI patients, and a POI mouse model. CXCL10 levels in serum and follicular fluid were higher in both bPOI and POI patients than in controls. An increased level of CXCL10 was also observed in a POI mouse model. CXCL10 concentrations in serum and follicular fluid were positively associated with follicle-stimulating hormone and negatively associated with antral follicle count. Our study for the first time found that CXCL10 induced COL1A1 and COL1A2 production, two subunits of collagen I in mouse theca–stroma cells by activating the JNK/c-Jun pathway. Inhibition of JNK and c-Jun attenuated the increases of COL1A1 and COL1A2 caused by CXCL10. Moreover, CXCL10 had no effects on hormone synthesis, proliferation, and apoptosis in human luteinized granulosa (hGL) cells. Our findings revealed a potential diagnostic value of CXCL10 in the early stage of POI and shed new insights into the biological function of CXCL10 in ovarian fibrosis. Frontiers Media S.A. 2022-04-01 /pmc/articles/PMC9010671/ /pubmed/35432206 http://dx.doi.org/10.3389/fendo.2022.823740 Text en Copyright © 2022 Wang and Sun https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Wang, Chaojun
Sun, Yun
Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway
title Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway
title_full Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway
title_fullStr Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway
title_full_unstemmed Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway
title_short Induction of Collagen I by CXCL10 in Ovarian Theca–Stroma Cells via the JNK Pathway
title_sort induction of collagen i by cxcl10 in ovarian theca–stroma cells via the jnk pathway
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010671/
https://www.ncbi.nlm.nih.gov/pubmed/35432206
http://dx.doi.org/10.3389/fendo.2022.823740
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