Cargando…
Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings. However, long-term clinical sample collection may cause batch effects that mask true biological results. Here, we present a validated and streamli...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010797/ https://www.ncbi.nlm.nih.gov/pubmed/35434658 http://dx.doi.org/10.1016/j.xpro.2022.101310 |
_version_ | 1784687561511272448 |
---|---|
author | Liu, Xiuxing Lv, Jianjie Wang, Huishi Zheng, Yingfeng Su, Wenru |
author_facet | Liu, Xiuxing Lv, Jianjie Wang, Huishi Zheng, Yingfeng Su, Wenru |
author_sort | Liu, Xiuxing |
collection | PubMed |
description | With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings. However, long-term clinical sample collection may cause batch effects that mask true biological results. Here, we present a validated and streamlined mass cytometry workflow that features fixed staining for clinical use and optimized barcode staining patterns. The reagents and approaches used in this workflow can help reduce batch effects, thereby extending the application range and advantages of mass cytometry. |
format | Online Article Text |
id | pubmed-9010797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90107972022-04-16 Functional analysis of human circulating immune cells based on high-dimensional mass cytometry Liu, Xiuxing Lv, Jianjie Wang, Huishi Zheng, Yingfeng Su, Wenru STAR Protoc Protocol With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings. However, long-term clinical sample collection may cause batch effects that mask true biological results. Here, we present a validated and streamlined mass cytometry workflow that features fixed staining for clinical use and optimized barcode staining patterns. The reagents and approaches used in this workflow can help reduce batch effects, thereby extending the application range and advantages of mass cytometry. Elsevier 2022-04-08 /pmc/articles/PMC9010797/ /pubmed/35434658 http://dx.doi.org/10.1016/j.xpro.2022.101310 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Liu, Xiuxing Lv, Jianjie Wang, Huishi Zheng, Yingfeng Su, Wenru Functional analysis of human circulating immune cells based on high-dimensional mass cytometry |
title | Functional analysis of human circulating immune cells based on high-dimensional mass cytometry |
title_full | Functional analysis of human circulating immune cells based on high-dimensional mass cytometry |
title_fullStr | Functional analysis of human circulating immune cells based on high-dimensional mass cytometry |
title_full_unstemmed | Functional analysis of human circulating immune cells based on high-dimensional mass cytometry |
title_short | Functional analysis of human circulating immune cells based on high-dimensional mass cytometry |
title_sort | functional analysis of human circulating immune cells based on high-dimensional mass cytometry |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010797/ https://www.ncbi.nlm.nih.gov/pubmed/35434658 http://dx.doi.org/10.1016/j.xpro.2022.101310 |
work_keys_str_mv | AT liuxiuxing functionalanalysisofhumancirculatingimmunecellsbasedonhighdimensionalmasscytometry AT lvjianjie functionalanalysisofhumancirculatingimmunecellsbasedonhighdimensionalmasscytometry AT wanghuishi functionalanalysisofhumancirculatingimmunecellsbasedonhighdimensionalmasscytometry AT zhengyingfeng functionalanalysisofhumancirculatingimmunecellsbasedonhighdimensionalmasscytometry AT suwenru functionalanalysisofhumancirculatingimmunecellsbasedonhighdimensionalmasscytometry |