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Functional analysis of human circulating immune cells based on high-dimensional mass cytometry

With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings. However, long-term clinical sample collection may cause batch effects that mask true biological results. Here, we present a validated and streamli...

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Detalles Bibliográficos
Autores principales: Liu, Xiuxing, Lv, Jianjie, Wang, Huishi, Zheng, Yingfeng, Su, Wenru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010797/
https://www.ncbi.nlm.nih.gov/pubmed/35434658
http://dx.doi.org/10.1016/j.xpro.2022.101310
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author Liu, Xiuxing
Lv, Jianjie
Wang, Huishi
Zheng, Yingfeng
Su, Wenru
author_facet Liu, Xiuxing
Lv, Jianjie
Wang, Huishi
Zheng, Yingfeng
Su, Wenru
author_sort Liu, Xiuxing
collection PubMed
description With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings. However, long-term clinical sample collection may cause batch effects that mask true biological results. Here, we present a validated and streamlined mass cytometry workflow that features fixed staining for clinical use and optimized barcode staining patterns. The reagents and approaches used in this workflow can help reduce batch effects, thereby extending the application range and advantages of mass cytometry.
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spelling pubmed-90107972022-04-16 Functional analysis of human circulating immune cells based on high-dimensional mass cytometry Liu, Xiuxing Lv, Jianjie Wang, Huishi Zheng, Yingfeng Su, Wenru STAR Protoc Protocol With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings. However, long-term clinical sample collection may cause batch effects that mask true biological results. Here, we present a validated and streamlined mass cytometry workflow that features fixed staining for clinical use and optimized barcode staining patterns. The reagents and approaches used in this workflow can help reduce batch effects, thereby extending the application range and advantages of mass cytometry. Elsevier 2022-04-08 /pmc/articles/PMC9010797/ /pubmed/35434658 http://dx.doi.org/10.1016/j.xpro.2022.101310 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Xiuxing
Lv, Jianjie
Wang, Huishi
Zheng, Yingfeng
Su, Wenru
Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
title Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
title_full Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
title_fullStr Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
title_full_unstemmed Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
title_short Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
title_sort functional analysis of human circulating immune cells based on high-dimensional mass cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010797/
https://www.ncbi.nlm.nih.gov/pubmed/35434658
http://dx.doi.org/10.1016/j.xpro.2022.101310
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