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Determining immunoglobulin-specific B cell receptor repertoire of murine splenocytes by next-generation sequencing

High-throughput antibody repertoire analysis via next-generation sequencing is a key method in understanding humoral immunity. Errors introduced during library preparation and sequencing can be corrected with molecular amplification fingerprinting using unique molecular identifiers. Here, we provide...

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Detalles Bibliográficos
Autores principales:	Werner, Anja, Schäfer, Simon, Gleußner, Nina, Nimmerjahn, Falk, Winkler, Thomas H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010798/ https://www.ncbi.nlm.nih.gov/pubmed/35434659 http://dx.doi.org/10.1016/j.xpro.2022.101277

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