Cargando…

Determining immunoglobulin-specific B cell receptor repertoire of murine splenocytes by next-generation sequencing

High-throughput antibody repertoire analysis via next-generation sequencing is a key method in understanding humoral immunity. Errors introduced during library preparation and sequencing can be corrected with molecular amplification fingerprinting using unique molecular identifiers. Here, we provide...

Descripción completa

Detalles Bibliográficos
Autores principales:	Werner, Anja, Schäfer, Simon, Gleußner, Nina, Nimmerjahn, Falk, Winkler, Thomas H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010798/ https://www.ncbi.nlm.nih.gov/pubmed/35434659 http://dx.doi.org/10.1016/j.xpro.2022.101277

Ejemplares similares

SYNTHESIS, INTRACELLULAR DISTRIBUTION, AND SECRETION OF IMMUNOGLOBULIN AND H-2 ANTIGEN IN MURINE SPLENOCYTES
por: Wernet, Dorothee, et al.
Publicado: (1973)

CELL SURFACE IMMUNOGLOBULIN : X. Identification of an IgD-Like Molecule on the Surface of Murine Splenocytes
por: Melcher, Ulrich, et al.
Publicado: (1974)

Next generation sequencing based assessment of the alloreactive T cell receptor repertoire in kidney transplant patients during rejection: a prospective cohort study
por: Aschauer, Constantin, et al.
Publicado: (2019)

CELL SURFACE IMMUNOGLOBULIN : IX. A NEW METHOD FOR THE STUDY OF SYNTHESIS, INTRACELLULAR TRANSPORT, AND EXTERIORIZATION IN MURINE SPLENOCYTES
por: Vitetta, Ellen S., et al.
Publicado: (1974)

Next-Generation Sequencing of Antibody Display Repertoires
por: Rouet, Romain, et al.
Publicado: (2018)

Immunoglobulin gene rearrangement in Koreans with multiple myeloma: Clonality assessment and repertoire analysis using next-generation sequencing
por: Kim, Miyoung, et al.
Publicado: (2021)

Next-Generation Sequencing Revealed a Distinct Immunoglobulin Repertoire with Specific Mutation Hotspots in Acute Myeloid Leukemia
por: Xia, Miaoran, et al.
Publicado: (2022)

Development and application of a next-generation sequencing protocol and bioinformatics pipeline for the comprehensive analysis of the canine immunoglobulin repertoire
por: Cullen, Jonah N., et al.
Publicado: (2022)

Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
por: Schulze, Thomas T., et al.
Publicado: (2022)

Protocol for the generation of murine bronchiolospheres
por: Vazquez-Armendariz, Ana Ivonne, et al.
Publicado: (2021)

The Application of Next Generation Sequencing to the Understanding of Antibody Repertoires
por: Mathonet, Pascale, et al.
Publicado: (2013)

AN ELECTROPHORETIC DIFFERENCE BETWEEN SURFACE AND SECRETED IGM OF MURINE SPLENOCYTES
por: Melcher, Ulrich, et al.
Publicado: (1973)

Suppression of IFN-γ Production in Murine Splenocytes by Histamine Receptor Antagonists
por: Kamei, Miho, et al.
Publicado: (2018)

Deletions of immunoglobulin C kappa region characterized by the circular excision products in mouse splenocytes
Publicado: (1991)

Targeting B cells in the pre-phase of systemic autoimmunity globally interferes with autoimmune pathology
por: Werner, Anja, et al.
Publicado: (2021)

Analyzing Immunoglobulin Repertoires
por: Chaudhary, Neha, et al.
Publicado: (2018)

Killer Immunoglobulin-Like Receptor Allele Determination Using Next-Generation Sequencing Technology
por: Maniangou, Bercelin, et al.
Publicado: (2017)

Immune Repertoire after Immunization As Seen by Next-Generation Sequencing and Proteomics
por: VanDuijn, Martijn M., et al.
Publicado: (2017)

Optimizing the cryopreservation of murine splenocytes for improved antigen-specific T cell function in ELISPOT
por: Gad, Ekram, et al.
Publicado: (2013)

The Past, Present, and Future of Immune Repertoire Biology – The Rise of Next-Generation Repertoire Analysis
por: Six, Adrien, et al.
Publicado: (2013)

Exonuclease activity and P nucleotide addition in the generation of the expressed immunoglobulin repertoire
por: Jackson, Katherine JL, et al.
Publicado: (2004)

The Effect of Enzymatically Polymerised Polyphenols on CD4 Binding and Cytokine Production in Murine Splenocytes
por: Yamanaka, Daisuke, et al.
Publicado: (2012)

Protective effects of strawberry and mulberry fruit polysaccharides on inflammation and apoptosis in murine primary splenocytes
por: Liu, Chieh-Jung, et al.
Publicado: (2014)

Repertoire of microRNAs in Epithelial Ovarian Cancer as Determined by Next Generation Sequencing of Small RNA cDNA Libraries
por: Wyman, Stacia K., et al.
Publicado: (2009)

Generation and functional characterization of murine mammary organoids
por: Yip, Hon Yan Kelvin, et al.
Publicado: (2021)

The effect of kynurenic acid on the synthesis of selected cytokines by murine splenocytes – in vitro and ex vivo studies
por: Małaczewska, Joanna, et al.
Publicado: (2016)

Optimisation of a murine splenocyte mycobacterial growth inhibition assay using virulent Mycobacterium tuberculosis
por: Jensen, Christina, et al.
Publicado: (2017)

Identification of disease-specific motifs in the antibody specificity repertoire via next-generation sequencing
por: Pantazes, Robert J., et al.
Publicado: (2016)

Repertoire of the gut microbiota from stomach to colon using culturomics and next-generation sequencing
por: Mailhe, Morgane, et al.
Publicado: (2018)

Quantitative assessment of the robustness of next-generation sequencing of antibody variable gene repertoires from immunized mice
por: Greiff, Victor, et al.
Publicado: (2014)

Generation of mixed murine organoids to model cellular interactions
por: Krotenberg Garcia, Ana, et al.
Publicado: (2021)

Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
por: Yang, Yang, et al.
Publicado: (2015)

T-cell receptor-α repertoire of CD8+ T cells following allogeneic stem cell transplantation using next-generation sequencing
por: Link-Rachner, Cornelia S., et al.
Publicado: (2019)

Murine gammaherpesvirus-68 expands, but does not activate, CD11b(+) gr-1(+) splenocytes in vivo
por: Nelson, Daniel A, et al.
Publicado: (2012)

Toll-like Receptor agonists and alpha-galactosylceramide synergistically enhance the production of interferon-gamma in murine splenocytes
por: Ando, Tatsuya, et al.
Publicado: (2013)

Glycosylation of immunoglobulin G determines osteoclast differentiation and bone loss
por: Harre, Ulrike, et al.
Publicado: (2015)

Pseudomonas-specific 16S rRNA insect gut-microbiome profiling using next-generation sequencing
por: Gupta, Ayushi, et al.
Publicado: (2022)

Next generation sequencing reveals changes of the γδ T cell receptor repertoires in patients with pulmonary tuberculosis
por: Cheng, Chaofei, et al.
Publicado: (2018)

Antibody response of mouse splenocytes using mixture of supernatants of thymocytes, adherent and non-adherent splenocytes: in-vitro immunization-II.
por: Kim, D., et al.
Publicado: (1990)

Toward a more accurate view of human B-cell repertoire by next-generation sequencing, unbiased repertoire capture and single-molecule barcoding
por: He, Linling, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_f0eq70eimfqc86j22m521i6lis