Cargando…
Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources
Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Urmia University Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010831/ https://www.ncbi.nlm.nih.gov/pubmed/35529810 http://dx.doi.org/10.30466/vrf.2020.110372.2618 |
_version_ | 1784687569316872192 |
---|---|
author | Barazandeh, Mohadeseh Shahin, Khashayar Hedayatkhah, Abolghasem Komijani, Majid Mansoorianfar, Mojtaba |
author_facet | Barazandeh, Mohadeseh Shahin, Khashayar Hedayatkhah, Abolghasem Komijani, Majid Mansoorianfar, Mojtaba |
author_sort | Barazandeh, Mohadeseh |
collection | PubMed |
description | Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (– 20.00 up to 70.00 ˚C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers. |
format | Online Article Text |
id | pubmed-9010831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Urmia University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-90108312022-05-05 Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources Barazandeh, Mohadeseh Shahin, Khashayar Hedayatkhah, Abolghasem Komijani, Majid Mansoorianfar, Mojtaba Vet Res Forum Original Article Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (– 20.00 up to 70.00 ˚C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers. Urmia University Press 2021-12 2021-12-15 /pmc/articles/PMC9010831/ /pubmed/35529810 http://dx.doi.org/10.30466/vrf.2020.110372.2618 Text en © 2021 Urmia University. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Barazandeh, Mohadeseh Shahin, Khashayar Hedayatkhah, Abolghasem Komijani, Majid Mansoorianfar, Mojtaba Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources |
title | Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources |
title_full | Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources |
title_fullStr | Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources |
title_full_unstemmed | Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources |
title_short | Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources |
title_sort | characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant pseudomonas aeruginosa isolated from human and domestic sources |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010831/ https://www.ncbi.nlm.nih.gov/pubmed/35529810 http://dx.doi.org/10.30466/vrf.2020.110372.2618 |
work_keys_str_mv | AT barazandehmohadeseh characterizationofanovelbulletshapedlyticbacteriophageagainstextensivelydrugresistantpseudomonasaeruginosaisolatedfromhumananddomesticsources AT shahinkhashayar characterizationofanovelbulletshapedlyticbacteriophageagainstextensivelydrugresistantpseudomonasaeruginosaisolatedfromhumananddomesticsources AT hedayatkhahabolghasem characterizationofanovelbulletshapedlyticbacteriophageagainstextensivelydrugresistantpseudomonasaeruginosaisolatedfromhumananddomesticsources AT komijanimajid characterizationofanovelbulletshapedlyticbacteriophageagainstextensivelydrugresistantpseudomonasaeruginosaisolatedfromhumananddomesticsources AT mansoorianfarmojtaba characterizationofanovelbulletshapedlyticbacteriophageagainstextensivelydrugresistantpseudomonasaeruginosaisolatedfromhumananddomesticsources |