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Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment

Listeria monocytogenes is one of the most important foodborne pathogens responsible for listeriosis, a severe disease with symptoms ranging from septicemia, meningoencephalitis, and abortion. Given the strong impact of listeriosis on human health and the difficulty of controlling L. monocytogenes al...

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Autores principales: Gattuso, Antonietta, Cella, Eleonora, Fillo, Silvia, Ortoffi, Marco Francesco, Angeletti, Silvia, Ciccozzi, Massimo, De Medici, Dario, Lista, Florigio, Fiore, Alfonsina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010861/
https://www.ncbi.nlm.nih.gov/pubmed/35432277
http://dx.doi.org/10.3389/fmicb.2022.816880
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author Gattuso, Antonietta
Cella, Eleonora
Fillo, Silvia
Ortoffi, Marco Francesco
Angeletti, Silvia
Ciccozzi, Massimo
De Medici, Dario
Lista, Florigio
Fiore, Alfonsina
author_facet Gattuso, Antonietta
Cella, Eleonora
Fillo, Silvia
Ortoffi, Marco Francesco
Angeletti, Silvia
Ciccozzi, Massimo
De Medici, Dario
Lista, Florigio
Fiore, Alfonsina
author_sort Gattuso, Antonietta
collection PubMed
description Listeria monocytogenes is one of the most important foodborne pathogens responsible for listeriosis, a severe disease with symptoms ranging from septicemia, meningoencephalitis, and abortion. Given the strong impact of listeriosis on human health and the difficulty of controlling L. monocytogenes along the food production chain, listeriosis has become a priority subjected to molecular surveillance in European Union/European Economic Area since 2007. From 2018, surveillance is based on whole-genome sequence using the core genome multilocus sequence type. The complete sequences of 132 clinical strains were used to define the evolutionary relatedness among subtypes of L. monocytogenes isolated in Italy from 2010 to 2016, allowing the identification of clades and/or clusters associated with outbreaks or sporadic cases of listeriosis. All the strains analyzed are clustered in lineages I and II, and the majority of the strains were classified as lineage II. A probable epidemic entrance in different years for every clade and cluster from each different region was defined. The persistence of the same specific clonal complexes of L. monocytogenes has been found over long periods; this may be related to the fact that some strains are able to survive better than others in a food production environment. Phylogenic studies, using whole-genome sequence data, are able to identify the emergence of highly persistent pathogenic variants, contributing to improving the hazard characterization of L. monocytogenes.
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spelling pubmed-90108612022-04-16 Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment Gattuso, Antonietta Cella, Eleonora Fillo, Silvia Ortoffi, Marco Francesco Angeletti, Silvia Ciccozzi, Massimo De Medici, Dario Lista, Florigio Fiore, Alfonsina Front Microbiol Microbiology Listeria monocytogenes is one of the most important foodborne pathogens responsible for listeriosis, a severe disease with symptoms ranging from septicemia, meningoencephalitis, and abortion. Given the strong impact of listeriosis on human health and the difficulty of controlling L. monocytogenes along the food production chain, listeriosis has become a priority subjected to molecular surveillance in European Union/European Economic Area since 2007. From 2018, surveillance is based on whole-genome sequence using the core genome multilocus sequence type. The complete sequences of 132 clinical strains were used to define the evolutionary relatedness among subtypes of L. monocytogenes isolated in Italy from 2010 to 2016, allowing the identification of clades and/or clusters associated with outbreaks or sporadic cases of listeriosis. All the strains analyzed are clustered in lineages I and II, and the majority of the strains were classified as lineage II. A probable epidemic entrance in different years for every clade and cluster from each different region was defined. The persistence of the same specific clonal complexes of L. monocytogenes has been found over long periods; this may be related to the fact that some strains are able to survive better than others in a food production environment. Phylogenic studies, using whole-genome sequence data, are able to identify the emergence of highly persistent pathogenic variants, contributing to improving the hazard characterization of L. monocytogenes. Frontiers Media S.A. 2022-04-01 /pmc/articles/PMC9010861/ /pubmed/35432277 http://dx.doi.org/10.3389/fmicb.2022.816880 Text en Copyright © 2022 Gattuso, Cella, Fillo, Ortoffi, Angeletti, Ciccozzi, De Medici, Lista and Fiore. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gattuso, Antonietta
Cella, Eleonora
Fillo, Silvia
Ortoffi, Marco Francesco
Angeletti, Silvia
Ciccozzi, Massimo
De Medici, Dario
Lista, Florigio
Fiore, Alfonsina
Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment
title Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment
title_full Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment
title_fullStr Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment
title_full_unstemmed Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment
title_short Phylogenetic and Evolutionary Genomic Analysis of Listeria monocytogenes Clinical Strains in the Framework of Foodborne Listeriosis Risk Assessment
title_sort phylogenetic and evolutionary genomic analysis of listeria monocytogenes clinical strains in the framework of foodborne listeriosis risk assessment
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010861/
https://www.ncbi.nlm.nih.gov/pubmed/35432277
http://dx.doi.org/10.3389/fmicb.2022.816880
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