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Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway

Although studies have shown that basic fibroblast growth factor (bFGF) can activate autophagy and promote peripheral nerve repair, the role and the molecular mechanism of action of bFGF in the facial nerve are not clear. In this study, a thermosensitive in situ forming poloxamer hydrogel was used as...

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Autores principales: Hu, Binbin, Zhang, Hanbo, Xu, Menglu, Li, Lei, Wu, Man, Zhang, Susu, Liu, Xuejun, Xia, Weidong, Xu, Ke, Xiao, Jian, Zhang, Hongyu, Ni, Liyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011134/
https://www.ncbi.nlm.nih.gov/pubmed/35431972
http://dx.doi.org/10.3389/fphar.2022.778680
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author Hu, Binbin
Zhang, Hanbo
Xu, Menglu
Li, Lei
Wu, Man
Zhang, Susu
Liu, Xuejun
Xia, Weidong
Xu, Ke
Xiao, Jian
Zhang, Hongyu
Ni, Liyan
author_facet Hu, Binbin
Zhang, Hanbo
Xu, Menglu
Li, Lei
Wu, Man
Zhang, Susu
Liu, Xuejun
Xia, Weidong
Xu, Ke
Xiao, Jian
Zhang, Hongyu
Ni, Liyan
author_sort Hu, Binbin
collection PubMed
description Although studies have shown that basic fibroblast growth factor (bFGF) can activate autophagy and promote peripheral nerve repair, the role and the molecular mechanism of action of bFGF in the facial nerve are not clear. In this study, a thermosensitive in situ forming poloxamer hydrogel was used as a vehicle to deliver bFGF for treating facial nerve injury (FNI) in the rat model. Using H&E and Masson’s staining, we found that bFGF hydrogel can promote the functional recovery and regeneration of the facial nerve. Furthermore, studies on the mechanism showed that bFGF can promote FNI recovery by promoting autophagy and inhibiting apoptosis. Additionally, this study demonstrated that the role of hydrogel binding bFGF in nerve repair was mediated through the activation of the PAK1 signaling pathway in Schwann cells (SCs). These results indicated that poloxamer thermosensitive hydrogel loaded with bFGF can significantly restore the morphology and function of the injured facial nerve by promoting autophagy and inhibiting apoptosis by activating the PAK1 pathway, which can provide a promising strategy for FNI recovery.
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spelling pubmed-90111342022-04-16 Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway Hu, Binbin Zhang, Hanbo Xu, Menglu Li, Lei Wu, Man Zhang, Susu Liu, Xuejun Xia, Weidong Xu, Ke Xiao, Jian Zhang, Hongyu Ni, Liyan Front Pharmacol Pharmacology Although studies have shown that basic fibroblast growth factor (bFGF) can activate autophagy and promote peripheral nerve repair, the role and the molecular mechanism of action of bFGF in the facial nerve are not clear. In this study, a thermosensitive in situ forming poloxamer hydrogel was used as a vehicle to deliver bFGF for treating facial nerve injury (FNI) in the rat model. Using H&E and Masson’s staining, we found that bFGF hydrogel can promote the functional recovery and regeneration of the facial nerve. Furthermore, studies on the mechanism showed that bFGF can promote FNI recovery by promoting autophagy and inhibiting apoptosis. Additionally, this study demonstrated that the role of hydrogel binding bFGF in nerve repair was mediated through the activation of the PAK1 signaling pathway in Schwann cells (SCs). These results indicated that poloxamer thermosensitive hydrogel loaded with bFGF can significantly restore the morphology and function of the injured facial nerve by promoting autophagy and inhibiting apoptosis by activating the PAK1 pathway, which can provide a promising strategy for FNI recovery. Frontiers Media S.A. 2022-04-01 /pmc/articles/PMC9011134/ /pubmed/35431972 http://dx.doi.org/10.3389/fphar.2022.778680 Text en Copyright © 2022 Hu, Zhang, Xu, Li, Wu, Zhang, Liu, Xia, Xu, Xiao, Zhang and Ni. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Hu, Binbin
Zhang, Hanbo
Xu, Menglu
Li, Lei
Wu, Man
Zhang, Susu
Liu, Xuejun
Xia, Weidong
Xu, Ke
Xiao, Jian
Zhang, Hongyu
Ni, Liyan
Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway
title Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway
title_full Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway
title_fullStr Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway
title_full_unstemmed Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway
title_short Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway
title_sort delivery of basic fibroblast growth factor through an in situ forming smart hydrogel activates autophagy in schwann cells and improves facial nerves generation via the pak-1 signaling pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011134/
https://www.ncbi.nlm.nih.gov/pubmed/35431972
http://dx.doi.org/10.3389/fphar.2022.778680
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