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Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking
BACKGROUND: Radix Scutellariae (RS) has been used to treat influenza for thousands of years in China. However, its mechanisms of action remain unclear. The aim of the present study was to use a network pharmacology and molecular docking-based approach to explore active components and potential molec...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011286/ https://www.ncbi.nlm.nih.gov/pubmed/35433930 http://dx.doi.org/10.21037/atm-22-1176 |
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author | Li, Qing Liu, Yuntao Yang, Min Jin, Lianshun Wu, Yali Tang, Lijuan He, Liuyun Wu, Dinghong Zhang, Zhongde |
author_facet | Li, Qing Liu, Yuntao Yang, Min Jin, Lianshun Wu, Yali Tang, Lijuan He, Liuyun Wu, Dinghong Zhang, Zhongde |
author_sort | Li, Qing |
collection | PubMed |
description | BACKGROUND: Radix Scutellariae (RS) has been used to treat influenza for thousands of years in China. However, its mechanisms of action remain unclear. The aim of the present study was to use a network pharmacology and molecular docking-based approach to explore active components and potential molecular mechanisms of RS for influenza A. METHODS: Target genes of RS and influenza A were attained by accessing network databases. We then determined the intersection of both genes through bioinformatics using R and Perl language. The protein-protein interaction (PPI) network was constructed by the STRING website (https://cn.string-db.org). The network analysis was done using Cytoscape software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were applied for the above genes. Effective components as core targets were screened out based on the condition that the interaction must come first. These core targets were combined with 3D structures of main RNA coding proteins of influenza A virus. Molecular docking was used to visualize drug–target interaction via AutoDock Vina and PyMOL. RESULTS: Twenty-eight active components and 40 target genes were acquired through the regulatory network of active components of RS and the PPI network. Seventy-one bioinformatics expressions were obtained through GO enrichment analysis (P<0.05). A total of 124 signaling pathways were screened by KEGG enrichment analysis (P<0.05). Acacetin, wogonin, baicalein, oroxylin A, and beta-sitosterol, which are rich in RS, are closely related to hemagglutinin (HA), NeurAminidase (NA), nucleoprotein (NP), polymerase basic protein 1 (PB1), polymerase basic protein 2 (PB2), polymerase acidic (PA), matrix protein 1 (M1), matrix protein 2 (M2), and non-structural protein (NS), which are the main RNA coding proteins of influenza A virus. The binding energies of these 8 proteins were less than –5 kJ/mol, indicating that the ligands had strong affinity with receptor proteins. CONCLUSIONS: RS is rich in core target compounds, and its mechanism of action is further expressed. It could have a good therapeutic effect for influenza A through multi-compound and multi-target regulation of these specific protein targets, and targets and pathways related to immunity and inflammation. |
format | Online Article Text |
id | pubmed-9011286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-90112862022-04-16 Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking Li, Qing Liu, Yuntao Yang, Min Jin, Lianshun Wu, Yali Tang, Lijuan He, Liuyun Wu, Dinghong Zhang, Zhongde Ann Transl Med Original Article BACKGROUND: Radix Scutellariae (RS) has been used to treat influenza for thousands of years in China. However, its mechanisms of action remain unclear. The aim of the present study was to use a network pharmacology and molecular docking-based approach to explore active components and potential molecular mechanisms of RS for influenza A. METHODS: Target genes of RS and influenza A were attained by accessing network databases. We then determined the intersection of both genes through bioinformatics using R and Perl language. The protein-protein interaction (PPI) network was constructed by the STRING website (https://cn.string-db.org). The network analysis was done using Cytoscape software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were applied for the above genes. Effective components as core targets were screened out based on the condition that the interaction must come first. These core targets were combined with 3D structures of main RNA coding proteins of influenza A virus. Molecular docking was used to visualize drug–target interaction via AutoDock Vina and PyMOL. RESULTS: Twenty-eight active components and 40 target genes were acquired through the regulatory network of active components of RS and the PPI network. Seventy-one bioinformatics expressions were obtained through GO enrichment analysis (P<0.05). A total of 124 signaling pathways were screened by KEGG enrichment analysis (P<0.05). Acacetin, wogonin, baicalein, oroxylin A, and beta-sitosterol, which are rich in RS, are closely related to hemagglutinin (HA), NeurAminidase (NA), nucleoprotein (NP), polymerase basic protein 1 (PB1), polymerase basic protein 2 (PB2), polymerase acidic (PA), matrix protein 1 (M1), matrix protein 2 (M2), and non-structural protein (NS), which are the main RNA coding proteins of influenza A virus. The binding energies of these 8 proteins were less than –5 kJ/mol, indicating that the ligands had strong affinity with receptor proteins. CONCLUSIONS: RS is rich in core target compounds, and its mechanism of action is further expressed. It could have a good therapeutic effect for influenza A through multi-compound and multi-target regulation of these specific protein targets, and targets and pathways related to immunity and inflammation. AME Publishing Company 2022-03 /pmc/articles/PMC9011286/ /pubmed/35433930 http://dx.doi.org/10.21037/atm-22-1176 Text en 2022 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Li, Qing Liu, Yuntao Yang, Min Jin, Lianshun Wu, Yali Tang, Lijuan He, Liuyun Wu, Dinghong Zhang, Zhongde Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking |
title | Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking |
title_full | Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking |
title_fullStr | Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking |
title_full_unstemmed | Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking |
title_short | Mechanism of Radix Scutellariae in the treatment of influenza A based on network pharmacology and molecular docking |
title_sort | mechanism of radix scutellariae in the treatment of influenza a based on network pharmacology and molecular docking |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011286/ https://www.ncbi.nlm.nih.gov/pubmed/35433930 http://dx.doi.org/10.21037/atm-22-1176 |
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