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Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike

Molecular imprinting has proven to be a versatile and simple strategy to obtain selective materials also termed “plastic antibodies” for a wide variety of species, i.e., from ions to macromolecules and viruses. However, to the best of the authors’ knowledge, the development of epitope‐imprinted poly...

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Autores principales: Batista, Alex D., Rajpal, Soumya, Keitel, Benedikt, Dietl, Sandra, Fresco‐Cala, Beatriz, Dinc, Mehmet, Groß, Rüdiger, Sobek, Harald, Münch, Jan, Mizaikoff, Boris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011513/
https://www.ncbi.nlm.nih.gov/pubmed/35441074
http://dx.doi.org/10.1002/admi.202101925
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author Batista, Alex D.
Rajpal, Soumya
Keitel, Benedikt
Dietl, Sandra
Fresco‐Cala, Beatriz
Dinc, Mehmet
Groß, Rüdiger
Sobek, Harald
Münch, Jan
Mizaikoff, Boris
author_facet Batista, Alex D.
Rajpal, Soumya
Keitel, Benedikt
Dietl, Sandra
Fresco‐Cala, Beatriz
Dinc, Mehmet
Groß, Rüdiger
Sobek, Harald
Münch, Jan
Mizaikoff, Boris
author_sort Batista, Alex D.
collection PubMed
description Molecular imprinting has proven to be a versatile and simple strategy to obtain selective materials also termed “plastic antibodies” for a wide variety of species, i.e., from ions to macromolecules and viruses. However, to the best of the authors’ knowledge, the development of epitope‐imprinted polymers for selective binding of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is not reported to date. An epitope from the SARS‐CoV‐2 spike protein comprising 17 amino acids is used as a template during the imprinting process. The interactions between the epitope template and organosilane monomers used for the polymer synthesis are predicted via molecular docking simulations. The molecularly imprinted polymer presents a 1.8‐fold higher selectivity against the target epitope compared to non‐imprinted control polymers. Rebinding studies with pseudoviruses containing SARS‐CoV‐2 spike protein demonstrate the superior selectivity of the molecularly imprinted matrices, which mimic the interactions of angiotensin‐converting enzyme 2 receptors from human cells. The obtained results highlight the potential of SARS‐CoV‐2 molecularly imprinted polymers for a variety of applications including chem/biosensing and antiviral delivery.
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spelling pubmed-90115132022-04-15 Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike Batista, Alex D. Rajpal, Soumya Keitel, Benedikt Dietl, Sandra Fresco‐Cala, Beatriz Dinc, Mehmet Groß, Rüdiger Sobek, Harald Münch, Jan Mizaikoff, Boris Adv Mater Interfaces Research Articles Molecular imprinting has proven to be a versatile and simple strategy to obtain selective materials also termed “plastic antibodies” for a wide variety of species, i.e., from ions to macromolecules and viruses. However, to the best of the authors’ knowledge, the development of epitope‐imprinted polymers for selective binding of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is not reported to date. An epitope from the SARS‐CoV‐2 spike protein comprising 17 amino acids is used as a template during the imprinting process. The interactions between the epitope template and organosilane monomers used for the polymer synthesis are predicted via molecular docking simulations. The molecularly imprinted polymer presents a 1.8‐fold higher selectivity against the target epitope compared to non‐imprinted control polymers. Rebinding studies with pseudoviruses containing SARS‐CoV‐2 spike protein demonstrate the superior selectivity of the molecularly imprinted matrices, which mimic the interactions of angiotensin‐converting enzyme 2 receptors from human cells. The obtained results highlight the potential of SARS‐CoV‐2 molecularly imprinted polymers for a variety of applications including chem/biosensing and antiviral delivery. John Wiley and Sons Inc. 2022-01-05 2022-02-14 /pmc/articles/PMC9011513/ /pubmed/35441074 http://dx.doi.org/10.1002/admi.202101925 Text en © 2022 The Authors. Advanced Materials Interfaces published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Batista, Alex D.
Rajpal, Soumya
Keitel, Benedikt
Dietl, Sandra
Fresco‐Cala, Beatriz
Dinc, Mehmet
Groß, Rüdiger
Sobek, Harald
Münch, Jan
Mizaikoff, Boris
Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike
title Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike
title_full Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike
title_fullStr Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike
title_full_unstemmed Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike
title_short Plastic Antibodies Mimicking the ACE2 Receptor for Selective Binding of SARS‐CoV‐2 Spike
title_sort plastic antibodies mimicking the ace2 receptor for selective binding of sars‐cov‐2 spike
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011513/
https://www.ncbi.nlm.nih.gov/pubmed/35441074
http://dx.doi.org/10.1002/admi.202101925
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