Cargando…
Measurement of procalcitonin in saliva of pigs: a pilot study
BACKGROUND: Procalcitonin (PCT) is a widely used biomarker of sepsis in human medicine and can have potential applications in the veterinary field. This study aimed to explore whether PCT could be measured in the saliva of pigs and whether its concentration changes in sepsis. Therefore, a specific a...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011990/ https://www.ncbi.nlm.nih.gov/pubmed/35428339 http://dx.doi.org/10.1186/s12917-022-03240-5 |
_version_ | 1784687711405211648 |
---|---|
author | López-Martínez, María José Escribano, Damián Martínez-Miró, Silvia Ramis, Guillermo Manzanilla, Edgar G. Tecles, Fernando Martínez-Subiela, Silvia Cerón, José J. |
author_facet | López-Martínez, María José Escribano, Damián Martínez-Miró, Silvia Ramis, Guillermo Manzanilla, Edgar G. Tecles, Fernando Martínez-Subiela, Silvia Cerón, José J. |
author_sort | López-Martínez, María José |
collection | PubMed |
description | BACKGROUND: Procalcitonin (PCT) is a widely used biomarker of sepsis in human medicine and can have potential applications in the veterinary field. This study aimed to explore whether PCT could be measured in the saliva of pigs and whether its concentration changes in sepsis. Therefore, a specific assay was developed and analytically validated, and changes in PCT concentration were evaluated in two conditions: a) in an experimental model of sepsis produced by the administration of lipopolysaccharide (LPS) to pigs (n = 5), that was compared with a model of non-septic inflammation induced by turpentine oil (n = 4), and b) in healthy piglets (n = 11) compared to piglets with meningitis (n = 20), a disease that usually involves sepsis and whose treatment often requires large amounts of antibiotics in farms. RESULTS: The assay showed coefficients of variation within the recommended limits and adequate linearity after serial sample dilutions. The method's detection limit was set at 68 μg/L, and the lower limit of quantification was 414 μg/L. In the LPS experiment, higher concentrations of PCT were found after 24 h in the animals injected with LPS (mean = 5790 μg/L) compared to those treated with turpentine oil (mean = 2127 μg/L, P = 0.045). Also, animals with meningitis had higher concentrations of PCT (mean = 21515 μg/L) than healthy pigs (mean = 6096 μg/L, P value < 0.0001). CONCLUSIONS: According to these results, this assay could be potentially used as a tool for the non-invasive detection of sepsis in pigs, which is currently a topic of high importance due to antibiotic use restriction. |
format | Online Article Text |
id | pubmed-9011990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90119902022-04-16 Measurement of procalcitonin in saliva of pigs: a pilot study López-Martínez, María José Escribano, Damián Martínez-Miró, Silvia Ramis, Guillermo Manzanilla, Edgar G. Tecles, Fernando Martínez-Subiela, Silvia Cerón, José J. BMC Vet Res Research Article BACKGROUND: Procalcitonin (PCT) is a widely used biomarker of sepsis in human medicine and can have potential applications in the veterinary field. This study aimed to explore whether PCT could be measured in the saliva of pigs and whether its concentration changes in sepsis. Therefore, a specific assay was developed and analytically validated, and changes in PCT concentration were evaluated in two conditions: a) in an experimental model of sepsis produced by the administration of lipopolysaccharide (LPS) to pigs (n = 5), that was compared with a model of non-septic inflammation induced by turpentine oil (n = 4), and b) in healthy piglets (n = 11) compared to piglets with meningitis (n = 20), a disease that usually involves sepsis and whose treatment often requires large amounts of antibiotics in farms. RESULTS: The assay showed coefficients of variation within the recommended limits and adequate linearity after serial sample dilutions. The method's detection limit was set at 68 μg/L, and the lower limit of quantification was 414 μg/L. In the LPS experiment, higher concentrations of PCT were found after 24 h in the animals injected with LPS (mean = 5790 μg/L) compared to those treated with turpentine oil (mean = 2127 μg/L, P = 0.045). Also, animals with meningitis had higher concentrations of PCT (mean = 21515 μg/L) than healthy pigs (mean = 6096 μg/L, P value < 0.0001). CONCLUSIONS: According to these results, this assay could be potentially used as a tool for the non-invasive detection of sepsis in pigs, which is currently a topic of high importance due to antibiotic use restriction. BioMed Central 2022-04-15 /pmc/articles/PMC9011990/ /pubmed/35428339 http://dx.doi.org/10.1186/s12917-022-03240-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article López-Martínez, María José Escribano, Damián Martínez-Miró, Silvia Ramis, Guillermo Manzanilla, Edgar G. Tecles, Fernando Martínez-Subiela, Silvia Cerón, José J. Measurement of procalcitonin in saliva of pigs: a pilot study |
title | Measurement of procalcitonin in saliva of pigs: a pilot study |
title_full | Measurement of procalcitonin in saliva of pigs: a pilot study |
title_fullStr | Measurement of procalcitonin in saliva of pigs: a pilot study |
title_full_unstemmed | Measurement of procalcitonin in saliva of pigs: a pilot study |
title_short | Measurement of procalcitonin in saliva of pigs: a pilot study |
title_sort | measurement of procalcitonin in saliva of pigs: a pilot study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011990/ https://www.ncbi.nlm.nih.gov/pubmed/35428339 http://dx.doi.org/10.1186/s12917-022-03240-5 |
work_keys_str_mv | AT lopezmartinezmariajose measurementofprocalcitonininsalivaofpigsapilotstudy AT escribanodamian measurementofprocalcitonininsalivaofpigsapilotstudy AT martinezmirosilvia measurementofprocalcitonininsalivaofpigsapilotstudy AT ramisguillermo measurementofprocalcitonininsalivaofpigsapilotstudy AT manzanillaedgarg measurementofprocalcitonininsalivaofpigsapilotstudy AT teclesfernando measurementofprocalcitonininsalivaofpigsapilotstudy AT martinezsubielasilvia measurementofprocalcitonininsalivaofpigsapilotstudy AT ceronjosej measurementofprocalcitonininsalivaofpigsapilotstudy |