Defective in utilizing glutathione 3, DUG3, is required for conidiation and host infection in the rice blast fungus Magnaporthe oryzae

Glutathione, an important redox buffer of the cell, also functions as a source of sulphur and nitrogen under starvation conditions. The metabolism and maintenance of glutathione homeostasis are vital for the appropriate functioning of the cell. In addition to the γ-glutamyl transpeptidase, the fungus-specific alternative pathway involving DUG1, DUG2 and DUG3 genes also mediate glutathione degradation. Here, we studied the functional significance of DUG3 in the vegetative growth and infection cycle of the cereal blast fungus Magnaporthe oryzae . Cells lacking the DUG3 gene displayed reduced conidiation, delayed appressorium formation, and a decrease in the severity of host infection. Further, we show that the γ-glutamyl transpeptidase inhibitor severely compromises the vegetative growth of the M. oryzae cells lacking the DUG3 gene. Taken together, our results suggest a significant role of glutathione metabolism in the growth and virulence of M. oryzae .