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Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA

Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and pro...

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Autores principales: Quach, Huy Quang, Ovsyannikova, Inna G., Poland, Gregory A., Kennedy, Richard B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013014/
https://www.ncbi.nlm.nih.gov/pubmed/35439529
http://dx.doi.org/10.1016/j.jim.2022.113275
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author Quach, Huy Quang
Ovsyannikova, Inna G.
Poland, Gregory A.
Kennedy, Richard B.
author_facet Quach, Huy Quang
Ovsyannikova, Inna G.
Poland, Gregory A.
Kennedy, Richard B.
author_sort Quach, Huy Quang
collection PubMed
description Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.
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spelling pubmed-90130142022-04-18 Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA Quach, Huy Quang Ovsyannikova, Inna G. Poland, Gregory A. Kennedy, Richard B. J Immunol Methods Article Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters. Elsevier B.V. 2022-06 2022-04-16 /pmc/articles/PMC9013014/ /pubmed/35439529 http://dx.doi.org/10.1016/j.jim.2022.113275 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Quach, Huy Quang
Ovsyannikova, Inna G.
Poland, Gregory A.
Kennedy, Richard B.
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA
title Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA
title_full Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA
title_fullStr Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA
title_full_unstemmed Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA
title_short Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA
title_sort detection of sars-cov-2 peptide-specific antibodies in syrian hamster serum by elisa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013014/
https://www.ncbi.nlm.nih.gov/pubmed/35439529
http://dx.doi.org/10.1016/j.jim.2022.113275
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