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Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP

Identification of resistant sources to Ascochyta blight (AB) has been considered as a main purpose in most chickpea breeding programs. Achievements to molecular markers related to resistance to Ascochyta rabiei allows selection programs to be developed more accurately and efficiently. The aim of thi...

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Autores principales: Zangene, Kiyanoush, Emamjomeh, Abbasali, Shokouhifar, Farhad, Mamarabadi, Mojtaba, Mehdinezhad, Nafiseh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013395/
https://www.ncbi.nlm.nih.gov/pubmed/35429254
http://dx.doi.org/10.1186/s13568-022-01385-y
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author Zangene, Kiyanoush
Emamjomeh, Abbasali
Shokouhifar, Farhad
Mamarabadi, Mojtaba
Mehdinezhad, Nafiseh
author_facet Zangene, Kiyanoush
Emamjomeh, Abbasali
Shokouhifar, Farhad
Mamarabadi, Mojtaba
Mehdinezhad, Nafiseh
author_sort Zangene, Kiyanoush
collection PubMed
description Identification of resistant sources to Ascochyta blight (AB) has been considered as a main purpose in most chickpea breeding programs. Achievements to molecular markers related to resistance to Ascochyta rabiei allows selection programs to be developed more accurately and efficiently. The aim of this study was to investigate the applicability of a functional SNP in differentiating Iranian resistant cultivars to be used in selection programs. Amplification of SNP-containing fragment with specific primer pair and its sequencing resulted in tracking and determining the allelic pattern of SNP18, SNP18-2147, SNP18-2491 and SNP18-2554 loci belong to GSH118 gene in ILC263 (sensitive) and MCC133 (resistant) chickpea lines. Mutations in SNP18 and SNP18-2147 occur at the protein level at positions 499 and 554. Bioinformatics studies have shown that the GSH118 gene is a Lucien-rich repeat receptor kinases (LRR-RKs) and encodes a membrane protein which can be involved in recognizing microorganisms and initiating immune signaling pathways in plants. Additional studies to determine the function of this gene and its interaction with other proteins can be effective in gaining more knowledge about the molecular basis of resistance against AB.
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spelling pubmed-90133952022-05-02 Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP Zangene, Kiyanoush Emamjomeh, Abbasali Shokouhifar, Farhad Mamarabadi, Mojtaba Mehdinezhad, Nafiseh AMB Express Original Article Identification of resistant sources to Ascochyta blight (AB) has been considered as a main purpose in most chickpea breeding programs. Achievements to molecular markers related to resistance to Ascochyta rabiei allows selection programs to be developed more accurately and efficiently. The aim of this study was to investigate the applicability of a functional SNP in differentiating Iranian resistant cultivars to be used in selection programs. Amplification of SNP-containing fragment with specific primer pair and its sequencing resulted in tracking and determining the allelic pattern of SNP18, SNP18-2147, SNP18-2491 and SNP18-2554 loci belong to GSH118 gene in ILC263 (sensitive) and MCC133 (resistant) chickpea lines. Mutations in SNP18 and SNP18-2147 occur at the protein level at positions 499 and 554. Bioinformatics studies have shown that the GSH118 gene is a Lucien-rich repeat receptor kinases (LRR-RKs) and encodes a membrane protein which can be involved in recognizing microorganisms and initiating immune signaling pathways in plants. Additional studies to determine the function of this gene and its interaction with other proteins can be effective in gaining more knowledge about the molecular basis of resistance against AB. Springer Berlin Heidelberg 2022-04-16 /pmc/articles/PMC9013395/ /pubmed/35429254 http://dx.doi.org/10.1186/s13568-022-01385-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Zangene, Kiyanoush
Emamjomeh, Abbasali
Shokouhifar, Farhad
Mamarabadi, Mojtaba
Mehdinezhad, Nafiseh
Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP
title Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP
title_full Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP
title_fullStr Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP
title_full_unstemmed Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP
title_short Differentiation of an Iranian resistance chickpea line to Ascochyta blight from a susceptible line using a functional SNP
title_sort differentiation of an iranian resistance chickpea line to ascochyta blight from a susceptible line using a functional snp
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013395/
https://www.ncbi.nlm.nih.gov/pubmed/35429254
http://dx.doi.org/10.1186/s13568-022-01385-y
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