Cargando…
Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis
Zebrafish is a popular research model; but its mechanism of sex determination is unclear and the sex of juvenile fish cannot be distinguished. To obtain fish with defined sex, we crossed domesticated zebrafish with the Nadia strain that has a female-dominant W segment. These fish were placed on a zi...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013747/ https://www.ncbi.nlm.nih.gov/pubmed/35445010 http://dx.doi.org/10.3389/fcell.2022.866267 |
_version_ | 1784688060903981056 |
---|---|
author | Pan, You-Jiun Tong, Sok-Keng Hsu, Chen-wei Weng, Jui-Hsia Chung, Bon-chu |
author_facet | Pan, You-Jiun Tong, Sok-Keng Hsu, Chen-wei Weng, Jui-Hsia Chung, Bon-chu |
author_sort | Pan, You-Jiun |
collection | PubMed |
description | Zebrafish is a popular research model; but its mechanism of sex determination is unclear and the sex of juvenile fish cannot be distinguished. To obtain fish with defined sex, we crossed domesticated zebrafish with the Nadia strain that has a female-dominant W segment. These fish were placed on a ziwi:GFP background to facilitate sorting of fluorescent germ cells for transcriptomic analysis. We analyzed the transcriptomes of germ cells at 10–14 days postfertilization (dpf), when sex dimorphic changes started to appear. Gene ontology showed that genes upregulated in the 10-dpf presumptive females are involved in cell cycles. This correlates with our detection of increased germ cell numbers and proliferation. We also detected upregulation of meiotic genes in the presumptive females at 14 dpf. Disruption of a meiotic gene, sycp3, resulted in sex reversal to infertile males. The germ cells of sycp3 mutants could not reach diplotene and underwent apoptosis. Preventing apoptosis by disrupting tp53 restored female characteristics in sycp3 mutants, demonstrating that adequate germ cells are required for female development. Thus, our transcriptome and gene mutation demonstrate that initial germ cell proliferation followed by meiosis is the hallmark of female differentiation in zebrafish. |
format | Online Article Text |
id | pubmed-9013747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90137472022-04-19 Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis Pan, You-Jiun Tong, Sok-Keng Hsu, Chen-wei Weng, Jui-Hsia Chung, Bon-chu Front Cell Dev Biol Cell and Developmental Biology Zebrafish is a popular research model; but its mechanism of sex determination is unclear and the sex of juvenile fish cannot be distinguished. To obtain fish with defined sex, we crossed domesticated zebrafish with the Nadia strain that has a female-dominant W segment. These fish were placed on a ziwi:GFP background to facilitate sorting of fluorescent germ cells for transcriptomic analysis. We analyzed the transcriptomes of germ cells at 10–14 days postfertilization (dpf), when sex dimorphic changes started to appear. Gene ontology showed that genes upregulated in the 10-dpf presumptive females are involved in cell cycles. This correlates with our detection of increased germ cell numbers and proliferation. We also detected upregulation of meiotic genes in the presumptive females at 14 dpf. Disruption of a meiotic gene, sycp3, resulted in sex reversal to infertile males. The germ cells of sycp3 mutants could not reach diplotene and underwent apoptosis. Preventing apoptosis by disrupting tp53 restored female characteristics in sycp3 mutants, demonstrating that adequate germ cells are required for female development. Thus, our transcriptome and gene mutation demonstrate that initial germ cell proliferation followed by meiosis is the hallmark of female differentiation in zebrafish. Frontiers Media S.A. 2022-04-04 /pmc/articles/PMC9013747/ /pubmed/35445010 http://dx.doi.org/10.3389/fcell.2022.866267 Text en Copyright © 2022 Pan, Tong, Hsu, Weng and Chung. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Pan, You-Jiun Tong, Sok-Keng Hsu, Chen-wei Weng, Jui-Hsia Chung, Bon-chu Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis |
title | Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis |
title_full | Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis |
title_fullStr | Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis |
title_full_unstemmed | Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis |
title_short | Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis |
title_sort | zebrafish establish female germ cell identity by advancing cell proliferation and meiosis |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013747/ https://www.ncbi.nlm.nih.gov/pubmed/35445010 http://dx.doi.org/10.3389/fcell.2022.866267 |
work_keys_str_mv | AT panyoujiun zebrafishestablishfemalegermcellidentitybyadvancingcellproliferationandmeiosis AT tongsokkeng zebrafishestablishfemalegermcellidentitybyadvancingcellproliferationandmeiosis AT hsuchenwei zebrafishestablishfemalegermcellidentitybyadvancingcellproliferationandmeiosis AT wengjuihsia zebrafishestablishfemalegermcellidentitybyadvancingcellproliferationandmeiosis AT chungbonchu zebrafishestablishfemalegermcellidentitybyadvancingcellproliferationandmeiosis |