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Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit

Vestibular deficit is a very common disorder in clinical practice and is characterized by vertigo, spontaneous nystagmus, and autonomic nervous symptoms, including nausea, vomiting, and sweating. In addition, the comorbidity of vestibular deficit and anxiety has long been an integral component of th...

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Autores principales: Li, Feitian, Feng, Yisi, Liu, Hongyan, Kong, Dedi, Hsueh, Chi-Yao, Shi, Xunbei, Wu, Qianru, Li, Wei, Wang, Jing, Zhang, Yibo, Dai, Chunfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013912/
https://www.ncbi.nlm.nih.gov/pubmed/35444956
http://dx.doi.org/10.3389/fcimb.2022.821780
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author Li, Feitian
Feng, Yisi
Liu, Hongyan
Kong, Dedi
Hsueh, Chi-Yao
Shi, Xunbei
Wu, Qianru
Li, Wei
Wang, Jing
Zhang, Yibo
Dai, Chunfu
author_facet Li, Feitian
Feng, Yisi
Liu, Hongyan
Kong, Dedi
Hsueh, Chi-Yao
Shi, Xunbei
Wu, Qianru
Li, Wei
Wang, Jing
Zhang, Yibo
Dai, Chunfu
author_sort Li, Feitian
collection PubMed
description Vestibular deficit is a very common disorder in clinical practice and is characterized by vertigo, spontaneous nystagmus, and autonomic nervous symptoms, including nausea, vomiting, and sweating. In addition, the comorbidity of vestibular deficit and anxiety has long been an integral component of the medical literature. Previous studies have suggested that the mechanisms underlying this comorbidity involved overlap of vestibular and cerebellar networks. Emerging evidence has shown that the microbiota–gut–brain axis plays a key role in the regulation of affective disorders. Thus, we hypothesized that the gut microbiota may be involved in the comorbidity of vestibular deficit and anxiety. To verify this, we constructed a unilateral labyrinthectomy mouse model to simulate vestibular deficit. Then, 16S rRNA gene sequencing and liquid chromatography–mass spectrometry (LC-MS) were used to analyze the microbiome and metabolome of the cecal samples collected from mice in the unilateral labyrinthectomy, sham surgery, and control groups. Notably, unilateral labyrinthectomy shaped the composition of the mouse gut microbiome, resulting in increased abundance of Lachnospiraceae NK4A136 group, Odoribacter and Roseburia and decreased abundance of Prevotella and Parasutterella at the genus level. Tax4Fun functional prediction indicated a decrease in tryptophan metabolism in mice in the unilateral labyrinthectomy group. Moreover, functional correlation of changes in gut microbes and metabolites between different groups showed that the oleamide level was negatively correlated with Odoribacter abundance (r = -0.89, p = 0.0002). The butyric acid level was positively correlated with Parasutterella abundance (r = 0.85, p = 0.0010). The propanoate level was negatively correlated with Prevotella abundance (r = -0.81, p = 0.0020). The 20-HETE level was positively correlated with Parasutterella abundance (r = 0.84, p = 0.0013). The altered microbes and metabolites were closely related to the pathogenesis of affective disorders. Our results not only offer novel insights into the vestibular deficit comorbid with anxiety but also build an important basis for future research on this etiology.
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spelling pubmed-90139122022-04-19 Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit Li, Feitian Feng, Yisi Liu, Hongyan Kong, Dedi Hsueh, Chi-Yao Shi, Xunbei Wu, Qianru Li, Wei Wang, Jing Zhang, Yibo Dai, Chunfu Front Cell Infect Microbiol Cellular and Infection Microbiology Vestibular deficit is a very common disorder in clinical practice and is characterized by vertigo, spontaneous nystagmus, and autonomic nervous symptoms, including nausea, vomiting, and sweating. In addition, the comorbidity of vestibular deficit and anxiety has long been an integral component of the medical literature. Previous studies have suggested that the mechanisms underlying this comorbidity involved overlap of vestibular and cerebellar networks. Emerging evidence has shown that the microbiota–gut–brain axis plays a key role in the regulation of affective disorders. Thus, we hypothesized that the gut microbiota may be involved in the comorbidity of vestibular deficit and anxiety. To verify this, we constructed a unilateral labyrinthectomy mouse model to simulate vestibular deficit. Then, 16S rRNA gene sequencing and liquid chromatography–mass spectrometry (LC-MS) were used to analyze the microbiome and metabolome of the cecal samples collected from mice in the unilateral labyrinthectomy, sham surgery, and control groups. Notably, unilateral labyrinthectomy shaped the composition of the mouse gut microbiome, resulting in increased abundance of Lachnospiraceae NK4A136 group, Odoribacter and Roseburia and decreased abundance of Prevotella and Parasutterella at the genus level. Tax4Fun functional prediction indicated a decrease in tryptophan metabolism in mice in the unilateral labyrinthectomy group. Moreover, functional correlation of changes in gut microbes and metabolites between different groups showed that the oleamide level was negatively correlated with Odoribacter abundance (r = -0.89, p = 0.0002). The butyric acid level was positively correlated with Parasutterella abundance (r = 0.85, p = 0.0010). The propanoate level was negatively correlated with Prevotella abundance (r = -0.81, p = 0.0020). The 20-HETE level was positively correlated with Parasutterella abundance (r = 0.84, p = 0.0013). The altered microbes and metabolites were closely related to the pathogenesis of affective disorders. Our results not only offer novel insights into the vestibular deficit comorbid with anxiety but also build an important basis for future research on this etiology. Frontiers Media S.A. 2022-04-04 /pmc/articles/PMC9013912/ /pubmed/35444956 http://dx.doi.org/10.3389/fcimb.2022.821780 Text en Copyright © 2022 Li, Feng, Liu, Kong, Hsueh, Shi, Wu, Li, Wang, Zhang and Dai https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Feitian
Feng, Yisi
Liu, Hongyan
Kong, Dedi
Hsueh, Chi-Yao
Shi, Xunbei
Wu, Qianru
Li, Wei
Wang, Jing
Zhang, Yibo
Dai, Chunfu
Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit
title Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit
title_full Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit
title_fullStr Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit
title_full_unstemmed Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit
title_short Gut Microbiome and Metabolome Changes in Mice With Acute Vestibular Deficit
title_sort gut microbiome and metabolome changes in mice with acute vestibular deficit
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013912/
https://www.ncbi.nlm.nih.gov/pubmed/35444956
http://dx.doi.org/10.3389/fcimb.2022.821780
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