Development and Application of CRISPR-Cas Based Tools

Abundant CRISPR-Cas systems in nature provide us with unlimited valuable resources to develop a variety of versatile tools, which are powerful weapons in biological discovery and disease treatment. Here, we systematically review the development of CRISPR-Cas based tools from DNA nuclease to RNA nucl...

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Detalles Bibliográficos
Autores principales: Hu, Yanping, Li, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013964/
https://www.ncbi.nlm.nih.gov/pubmed/35445018
http://dx.doi.org/10.3389/fcell.2022.834646
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author Hu, Yanping
Li, Wei
author_facet Hu, Yanping
Li, Wei
author_sort Hu, Yanping
collection PubMed
description Abundant CRISPR-Cas systems in nature provide us with unlimited valuable resources to develop a variety of versatile tools, which are powerful weapons in biological discovery and disease treatment. Here, we systematically review the development of CRISPR-Cas based tools from DNA nuclease to RNA nuclease, from nuclease dependent-tools to nucleic acid recognition dependent-tools. Also, considering the limitations and challenges of current CRISPR-Cas based tools, we discuss the potential directions for development of novel CRISPR toolkits in the future.
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spelling pubmed-90139642022-04-19 Development and Application of CRISPR-Cas Based Tools Hu, Yanping Li, Wei Front Cell Dev Biol Cell and Developmental Biology Abundant CRISPR-Cas systems in nature provide us with unlimited valuable resources to develop a variety of versatile tools, which are powerful weapons in biological discovery and disease treatment. Here, we systematically review the development of CRISPR-Cas based tools from DNA nuclease to RNA nuclease, from nuclease dependent-tools to nucleic acid recognition dependent-tools. Also, considering the limitations and challenges of current CRISPR-Cas based tools, we discuss the potential directions for development of novel CRISPR toolkits in the future. Frontiers Media S.A. 2022-04-04 /pmc/articles/PMC9013964/ /pubmed/35445018 http://dx.doi.org/10.3389/fcell.2022.834646 Text en Copyright © 2022 Hu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Hu, Yanping
Li, Wei
Development and Application of CRISPR-Cas Based Tools
title Development and Application of CRISPR-Cas Based Tools
title_full Development and Application of CRISPR-Cas Based Tools
title_fullStr Development and Application of CRISPR-Cas Based Tools
title_full_unstemmed Development and Application of CRISPR-Cas Based Tools
title_short Development and Application of CRISPR-Cas Based Tools
title_sort development and application of crispr-cas based tools
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013964/
https://www.ncbi.nlm.nih.gov/pubmed/35445018
http://dx.doi.org/10.3389/fcell.2022.834646
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