Cargando…

Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection

Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostre...

Descripción completa

Detalles Bibliográficos
Autores principales: Picot, Sandy, Faury, Nicole, Pelletier, Camille, Arzul, Isabelle, Chollet, Bruno, Dégremont, Lionel, Renault, Tristan, Morga, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014014/
https://www.ncbi.nlm.nih.gov/pubmed/35444958
http://dx.doi.org/10.3389/fcimb.2022.858311
_version_ 1784688123348779008
author Picot, Sandy
Faury, Nicole
Pelletier, Camille
Arzul, Isabelle
Chollet, Bruno
Dégremont, Lionel
Renault, Tristan
Morga, Benjamin
author_facet Picot, Sandy
Faury, Nicole
Pelletier, Camille
Arzul, Isabelle
Chollet, Bruno
Dégremont, Lionel
Renault, Tristan
Morga, Benjamin
author_sort Picot, Sandy
collection PubMed
description Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas, recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1.
format Online
Article
Text
id pubmed-9014014
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90140142022-04-19 Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin Front Cell Infect Microbiol Cellular and Infection Microbiology Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas, recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1. Frontiers Media S.A. 2022-04-04 /pmc/articles/PMC9014014/ /pubmed/35444958 http://dx.doi.org/10.3389/fcimb.2022.858311 Text en Copyright © 2022 Picot, Faury, Pelletier, Arzul, Chollet, Dégremont, Renault and Morga https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Picot, Sandy
Faury, Nicole
Pelletier, Camille
Arzul, Isabelle
Chollet, Bruno
Dégremont, Lionel
Renault, Tristan
Morga, Benjamin
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
title Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
title_full Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
title_fullStr Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
title_full_unstemmed Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
title_short Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
title_sort monitoring autophagy at cellular and molecular level in crassostrea gigas during an experimental ostreid herpesvirus 1 (oshv-1) infection
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014014/
https://www.ncbi.nlm.nih.gov/pubmed/35444958
http://dx.doi.org/10.3389/fcimb.2022.858311
work_keys_str_mv AT picotsandy monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection
AT faurynicole monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection
AT pelletiercamille monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection
AT arzulisabelle monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection
AT cholletbruno monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection
AT degremontlionel monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection
AT renaulttristan monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection
AT morgabenjamin monitoringautophagyatcellularandmolecularlevelincrassostreagigasduringanexperimentalostreidherpesvirus1oshv1infection