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Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs

Trained immunity is the capacity of innate immune cells to produce an improved response against a secondary infection after a previous unrelated infection. Salmonellosis represents a public health issue and affects the pig farming industry. In general, vaccination against salmonellosis is still faci...

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Autores principales: Vaz-Rodrigues, Rita, Ferreras-Colino, Elisa, Ugarte-Ruíz, María, Pesciaroli, Michele, Thomas, Jobin, García-Seco, Teresa, Sevilla, Iker A., Pérez-Sancho, Marta, Mateo, Rafael, Domínguez, Lucas, Gortazar, Christian, Risalde, María A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014587/
https://www.ncbi.nlm.nih.gov/pubmed/35436975
http://dx.doi.org/10.1186/s13567-022-01047-8
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author Vaz-Rodrigues, Rita
Ferreras-Colino, Elisa
Ugarte-Ruíz, María
Pesciaroli, Michele
Thomas, Jobin
García-Seco, Teresa
Sevilla, Iker A.
Pérez-Sancho, Marta
Mateo, Rafael
Domínguez, Lucas
Gortazar, Christian
Risalde, María A.
author_facet Vaz-Rodrigues, Rita
Ferreras-Colino, Elisa
Ugarte-Ruíz, María
Pesciaroli, Michele
Thomas, Jobin
García-Seco, Teresa
Sevilla, Iker A.
Pérez-Sancho, Marta
Mateo, Rafael
Domínguez, Lucas
Gortazar, Christian
Risalde, María A.
author_sort Vaz-Rodrigues, Rita
collection PubMed
description Trained immunity is the capacity of innate immune cells to produce an improved response against a secondary infection after a previous unrelated infection. Salmonellosis represents a public health issue and affects the pig farming industry. In general, vaccination against salmonellosis is still facing problems regarding the control of distinct serovars. Therefore, we hypothesized that an immunostimulant based on heat inactivated Mycobacterium bovis (HIMB) could have an immune training effect in pigs challenged with Salmonella enterica serovar Choleraesuis (S. Choleraesuis) and decided to explore the amplitude of this non-specific immune response. For this purpose, twenty-four 10 days-old female piglets were randomly separated in three groups: immunized group (n = 10) received orally two doses of HIMB prior to the intratracheal S. Choleraesuis-challenge, positive control group (n = 9) that was only challenged with S. Choleraesuis, and negative control group (n = 5) that was neither immunized nor infected. All individuals were necropsied 21 days post-challenge. HIMB improved weight gain and reduced respiratory symptoms and pulmonary lesions caused by S. Choleraesuis in pigs. Pigs immunized with HIMB showed higher cytokine production, especially of serum TNFα and lung CCL28, an important mediator of mucosal trained immunity. Moreover, immunized pigs showed lower levels of the biomarker of lipid oxidation malondialdehyde and higher activity of the antioxidant enzyme superoxide dismutase than untreated challenged pigs. However, the excretion and tissue colonization of S. Choleraesuis remained unaffected. This proof-of-concept study suggests beneficial clinical, pathological, and heterologous immunological effects against bacterial pathogens within the concept of trained immunity, opening avenues for further research.
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spelling pubmed-90145872022-04-19 Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs Vaz-Rodrigues, Rita Ferreras-Colino, Elisa Ugarte-Ruíz, María Pesciaroli, Michele Thomas, Jobin García-Seco, Teresa Sevilla, Iker A. Pérez-Sancho, Marta Mateo, Rafael Domínguez, Lucas Gortazar, Christian Risalde, María A. Vet Res Research Article Trained immunity is the capacity of innate immune cells to produce an improved response against a secondary infection after a previous unrelated infection. Salmonellosis represents a public health issue and affects the pig farming industry. In general, vaccination against salmonellosis is still facing problems regarding the control of distinct serovars. Therefore, we hypothesized that an immunostimulant based on heat inactivated Mycobacterium bovis (HIMB) could have an immune training effect in pigs challenged with Salmonella enterica serovar Choleraesuis (S. Choleraesuis) and decided to explore the amplitude of this non-specific immune response. For this purpose, twenty-four 10 days-old female piglets were randomly separated in three groups: immunized group (n = 10) received orally two doses of HIMB prior to the intratracheal S. Choleraesuis-challenge, positive control group (n = 9) that was only challenged with S. Choleraesuis, and negative control group (n = 5) that was neither immunized nor infected. All individuals were necropsied 21 days post-challenge. HIMB improved weight gain and reduced respiratory symptoms and pulmonary lesions caused by S. Choleraesuis in pigs. Pigs immunized with HIMB showed higher cytokine production, especially of serum TNFα and lung CCL28, an important mediator of mucosal trained immunity. Moreover, immunized pigs showed lower levels of the biomarker of lipid oxidation malondialdehyde and higher activity of the antioxidant enzyme superoxide dismutase than untreated challenged pigs. However, the excretion and tissue colonization of S. Choleraesuis remained unaffected. This proof-of-concept study suggests beneficial clinical, pathological, and heterologous immunological effects against bacterial pathogens within the concept of trained immunity, opening avenues for further research. BioMed Central 2022-04-18 2022 /pmc/articles/PMC9014587/ /pubmed/35436975 http://dx.doi.org/10.1186/s13567-022-01047-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Vaz-Rodrigues, Rita
Ferreras-Colino, Elisa
Ugarte-Ruíz, María
Pesciaroli, Michele
Thomas, Jobin
García-Seco, Teresa
Sevilla, Iker A.
Pérez-Sancho, Marta
Mateo, Rafael
Domínguez, Lucas
Gortazar, Christian
Risalde, María A.
Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs
title Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs
title_full Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs
title_fullStr Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs
title_full_unstemmed Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs
title_short Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs
title_sort nonspecific protection of heat-inactivated mycobacterium bovis against salmonella choleraesuis infection in pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014587/
https://www.ncbi.nlm.nih.gov/pubmed/35436975
http://dx.doi.org/10.1186/s13567-022-01047-8
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