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Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses

The 5′UTR part of coronavirus genomes plays key roles in the viral replication cycle and translation of viral mRNAs. The first 75–80 nt, also called the leader sequence, are identical for genomic mRNA and subgenomic mRNAs. Recently, it was shown that cooperative actions of a 5′UTR segment and the no...

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Autores principales: Sosnowski, Piotr, Tidu, Antonin, Eriani, Gilbert, Westhof, Eric, Martin, Franck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014872/
https://www.ncbi.nlm.nih.gov/pubmed/35236777
http://dx.doi.org/10.1261/rna.078972.121
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author Sosnowski, Piotr
Tidu, Antonin
Eriani, Gilbert
Westhof, Eric
Martin, Franck
author_facet Sosnowski, Piotr
Tidu, Antonin
Eriani, Gilbert
Westhof, Eric
Martin, Franck
author_sort Sosnowski, Piotr
collection PubMed
description The 5′UTR part of coronavirus genomes plays key roles in the viral replication cycle and translation of viral mRNAs. The first 75–80 nt, also called the leader sequence, are identical for genomic mRNA and subgenomic mRNAs. Recently, it was shown that cooperative actions of a 5′UTR segment and the nonstructural protein NSP1 are essential for both the inhibition of host mRNAs and for specific translation of viral mRNAs. Here, sequence analyses of both the 5′UTR RNA segment and the NSP1 protein have been done for several coronaviruses, with special attention to the betacoronaviruses. The conclusions are: (i) precise specific molecular signatures can be found in both the RNA and the NSP1 protein; (ii) both types of signatures correlate between each other. Indeed, definite sequence motifs in the RNA correlate with sequence motifs in the protein, indicating a coevolution between the 5′UTR and NSP1 in betacoronaviruses. Experimental mutational data on 5′UTR and NSP1 from SARS-CoV-2 using cell-free translation extracts support these conclusions and show that some conserved key residues in the amino-terminal half of the NSP1 protein are essential for evasion to the inhibitory effect of NSP1 on translation.
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spelling pubmed-90148722022-05-02 Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses Sosnowski, Piotr Tidu, Antonin Eriani, Gilbert Westhof, Eric Martin, Franck RNA Article The 5′UTR part of coronavirus genomes plays key roles in the viral replication cycle and translation of viral mRNAs. The first 75–80 nt, also called the leader sequence, are identical for genomic mRNA and subgenomic mRNAs. Recently, it was shown that cooperative actions of a 5′UTR segment and the nonstructural protein NSP1 are essential for both the inhibition of host mRNAs and for specific translation of viral mRNAs. Here, sequence analyses of both the 5′UTR RNA segment and the NSP1 protein have been done for several coronaviruses, with special attention to the betacoronaviruses. The conclusions are: (i) precise specific molecular signatures can be found in both the RNA and the NSP1 protein; (ii) both types of signatures correlate between each other. Indeed, definite sequence motifs in the RNA correlate with sequence motifs in the protein, indicating a coevolution between the 5′UTR and NSP1 in betacoronaviruses. Experimental mutational data on 5′UTR and NSP1 from SARS-CoV-2 using cell-free translation extracts support these conclusions and show that some conserved key residues in the amino-terminal half of the NSP1 protein are essential for evasion to the inhibitory effect of NSP1 on translation. Cold Spring Harbor Laboratory Press 2022-05 /pmc/articles/PMC9014872/ /pubmed/35236777 http://dx.doi.org/10.1261/rna.078972.121 Text en © 2022 Sosnowski et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Article
Sosnowski, Piotr
Tidu, Antonin
Eriani, Gilbert
Westhof, Eric
Martin, Franck
Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses
title Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses
title_full Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses
title_fullStr Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses
title_full_unstemmed Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses
title_short Correlated sequence signatures are present within the genomic 5′UTR RNA and NSP1 protein in coronaviruses
title_sort correlated sequence signatures are present within the genomic 5′utr rna and nsp1 protein in coronaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014872/
https://www.ncbi.nlm.nih.gov/pubmed/35236777
http://dx.doi.org/10.1261/rna.078972.121
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