Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index

Radiotherapy is one of the most important options for treating breast cancer in humans. The development of biomarkers to monitor radiosensitivity is scarce. The aim of this study is to investigate the γH2AX levels in the human blood samples 0.5 h after radiotherapy compared to the levels before radi...

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Autores principales: Mahmoud, Alkhansa S., E. Hassan, Ammar M., Ali, Amna A., Hassan, Nuha M., Yousif, Amna A., Elbashir, Fawzia E., Omer, Ahmed, Abdalla, Omer M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015078/
https://www.ncbi.nlm.nih.gov/pubmed/35444787
http://dx.doi.org/10.4103/genint.genint_1_22
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author Mahmoud, Alkhansa S.
E. Hassan, Ammar M.
Ali, Amna A.
Hassan, Nuha M.
Yousif, Amna A.
Elbashir, Fawzia E.
Omer, Ahmed
Abdalla, Omer M.
author_facet Mahmoud, Alkhansa S.
E. Hassan, Ammar M.
Ali, Amna A.
Hassan, Nuha M.
Yousif, Amna A.
Elbashir, Fawzia E.
Omer, Ahmed
Abdalla, Omer M.
author_sort Mahmoud, Alkhansa S.
collection PubMed
description Radiotherapy is one of the most important options for treating breast cancer in humans. The development of biomarkers to monitor radiosensitivity is scarce. The aim of this study is to investigate the γH2AX levels in the human blood samples 0.5 h after radiotherapy compared to the levels before radiotherapy in breast cancer patients in relation to their respective body mass index (BMI). Blood plasma samples were collected from a total of 20 breast cancer patients before and after radiotherapy to measure γH2AX levels with an antibody against γH2AX based on enzyme-linked immunosorbent assay technique. The median BMI of the patients was 30 kg/m(2). γH2AX was differentially expressed in breast cancer patients before radiotherapy. γH2AX levels significantly increased in 14 patients after radiotherapy (P = 0.006), whereas γH2AX levels decreased in three patients after radiotherapy, and three patients were excluded. There was no correlation between γH2AX values after radiotherapy and BMI (P = 0.5, r = 0.1). Our results suggest that γH2AX can be used by ELISA technique to measure γH2AX in the blood plasma of breast cancer patients undergoing radiotherapy and can be considered a biomarker of radiosensitivity.
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spelling pubmed-90150782022-04-19 Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index Mahmoud, Alkhansa S. E. Hassan, Ammar M. Ali, Amna A. Hassan, Nuha M. Yousif, Amna A. Elbashir, Fawzia E. Omer, Ahmed Abdalla, Omer M. Genome Integr Original Article Radiotherapy is one of the most important options for treating breast cancer in humans. The development of biomarkers to monitor radiosensitivity is scarce. The aim of this study is to investigate the γH2AX levels in the human blood samples 0.5 h after radiotherapy compared to the levels before radiotherapy in breast cancer patients in relation to their respective body mass index (BMI). Blood plasma samples were collected from a total of 20 breast cancer patients before and after radiotherapy to measure γH2AX levels with an antibody against γH2AX based on enzyme-linked immunosorbent assay technique. The median BMI of the patients was 30 kg/m(2). γH2AX was differentially expressed in breast cancer patients before radiotherapy. γH2AX levels significantly increased in 14 patients after radiotherapy (P = 0.006), whereas γH2AX levels decreased in three patients after radiotherapy, and three patients were excluded. There was no correlation between γH2AX values after radiotherapy and BMI (P = 0.5, r = 0.1). Our results suggest that γH2AX can be used by ELISA technique to measure γH2AX in the blood plasma of breast cancer patients undergoing radiotherapy and can be considered a biomarker of radiosensitivity. Wolters Kluwer - Medknow 2022-03-24 /pmc/articles/PMC9015078/ /pubmed/35444787 http://dx.doi.org/10.4103/genint.genint_1_22 Text en Copyright: © 2022 Genome Integrity https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Mahmoud, Alkhansa S.
E. Hassan, Ammar M.
Ali, Amna A.
Hassan, Nuha M.
Yousif, Amna A.
Elbashir, Fawzia E.
Omer, Ahmed
Abdalla, Omer M.
Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index
title Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index
title_full Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index
title_fullStr Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index
title_full_unstemmed Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index
title_short Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index
title_sort detection of radiation-induced dna damage in breast cancer patients by using gamma h2ax biomarker: a possible correlation with their body mass index
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015078/
https://www.ncbi.nlm.nih.gov/pubmed/35444787
http://dx.doi.org/10.4103/genint.genint_1_22
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