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Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model

We previously developed a non-cell-dependent biodegradable scaffold to create in situ tissue-engineered vasculature (iTEV) and tested it in a canine inferior vena cava (IVC) model. As iTEV features change dramatically during tissue generation, practical, simple, and accurate methods to evaluate iTEV...

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Detalles Bibliográficos
Autores principales: Matsumura, Goki, Isayama, Noriko, Sato, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015146/
https://www.ncbi.nlm.nih.gov/pubmed/35436313
http://dx.doi.org/10.1371/journal.pone.0267274
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author Matsumura, Goki
Isayama, Noriko
Sato, Hideki
author_facet Matsumura, Goki
Isayama, Noriko
Sato, Hideki
author_sort Matsumura, Goki
collection PubMed
description We previously developed a non-cell-dependent biodegradable scaffold to create in situ tissue-engineered vasculature (iTEV) and tested it in a canine inferior vena cava (IVC) model. As iTEV features change dramatically during tissue generation, practical, simple, and accurate methods to evaluate iTEV are needed. The present study examined the usefulness of a novel method to evaluate iTEV growth and remodeling according to a simple formula using angiography: hepatic vein (HV) index = (IVC–HV junction angle) ÷ (π × [minimal internal iTEV diameter ÷ 2](2)). HV index strongly correlated with the pressure gradient across iTEV, which tended to improve during the tissue generation period up to 12 months post-implantation. Time-course changes in HV index reflected iTEV tissue development and in-vivo characteristics, such as hemodynamic congestion. In conclusion, HV index is useful to assess iTEV graft function because it represents both the morphometrics and hemodynamics of iTEV with only diagnostic imaging data.
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spelling pubmed-90151462022-04-19 Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model Matsumura, Goki Isayama, Noriko Sato, Hideki PLoS One Research Article We previously developed a non-cell-dependent biodegradable scaffold to create in situ tissue-engineered vasculature (iTEV) and tested it in a canine inferior vena cava (IVC) model. As iTEV features change dramatically during tissue generation, practical, simple, and accurate methods to evaluate iTEV are needed. The present study examined the usefulness of a novel method to evaluate iTEV growth and remodeling according to a simple formula using angiography: hepatic vein (HV) index = (IVC–HV junction angle) ÷ (π × [minimal internal iTEV diameter ÷ 2](2)). HV index strongly correlated with the pressure gradient across iTEV, which tended to improve during the tissue generation period up to 12 months post-implantation. Time-course changes in HV index reflected iTEV tissue development and in-vivo characteristics, such as hemodynamic congestion. In conclusion, HV index is useful to assess iTEV graft function because it represents both the morphometrics and hemodynamics of iTEV with only diagnostic imaging data. Public Library of Science 2022-04-18 /pmc/articles/PMC9015146/ /pubmed/35436313 http://dx.doi.org/10.1371/journal.pone.0267274 Text en © 2022 Matsumura et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Matsumura, Goki
Isayama, Noriko
Sato, Hideki
Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
title Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
title_full Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
title_fullStr Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
title_full_unstemmed Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
title_short Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
title_sort evaluation method for cell-free in situ tissue-engineered vasculature monitoring: proof of growth and development in a canine ivc model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015146/
https://www.ncbi.nlm.nih.gov/pubmed/35436313
http://dx.doi.org/10.1371/journal.pone.0267274
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