Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL

LIN-41 (TRIM71), an ancient protein best known for its role in timing mitotic stem cell lineages, has been recently shown to be involved in postmitotic neurons to time their differentiation and post-differentiation. Here, we report the identification of 276 LIN-41 protein-expressing neurons in the C. elegans nervous system by NeuroPAL and a CRISPR-engineered mNG::LIN-41 reporter, which represents 91% of all hermaphrodite neurons and includes 87 neurons that were not previously reported by CeNGEN using single-cell RNA-seq. Broad lin-41 protein expression in C. elegans neurons suggests a widespread role of LIN-41 (TRIM71) in timing neuronal assembly, plasticity, and maintenance.