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Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL
LIN-41 (TRIM71), an ancient protein best known for its role in timing mitotic stem cell lineages, has been recently shown to be involved in postmitotic neurons to time their differentiation and post-differentiation. Here, we report the identification of 276 LIN-41 protein-expressing neurons in the C...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015712/ https://www.ncbi.nlm.nih.gov/pubmed/35622497 http://dx.doi.org/10.17912/micropub.biology.000472 |
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author | Shih, Mushaine Chang, Chieh |
author_facet | Shih, Mushaine Chang, Chieh |
author_sort | Shih, Mushaine |
collection | PubMed |
description | LIN-41 (TRIM71), an ancient protein best known for its role in timing mitotic stem cell lineages, has been recently shown to be involved in postmitotic neurons to time their differentiation and post-differentiation. Here, we report the identification of 276 LIN-41 protein-expressing neurons in the C. elegans nervous system by NeuroPAL and a CRISPR-engineered mNG::LIN-41 reporter, which represents 91% of all hermaphrodite neurons and includes 87 neurons that were not previously reported by CeNGEN using single-cell RNA-seq. Broad lin-41 protein expression in C. elegans neurons suggests a widespread role of LIN-41 (TRIM71) in timing neuronal assembly, plasticity, and maintenance. |
format | Online Article Text |
id | pubmed-9015712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-90157122022-04-19 Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL Shih, Mushaine Chang, Chieh MicroPubl Biol New Finding LIN-41 (TRIM71), an ancient protein best known for its role in timing mitotic stem cell lineages, has been recently shown to be involved in postmitotic neurons to time their differentiation and post-differentiation. Here, we report the identification of 276 LIN-41 protein-expressing neurons in the C. elegans nervous system by NeuroPAL and a CRISPR-engineered mNG::LIN-41 reporter, which represents 91% of all hermaphrodite neurons and includes 87 neurons that were not previously reported by CeNGEN using single-cell RNA-seq. Broad lin-41 protein expression in C. elegans neurons suggests a widespread role of LIN-41 (TRIM71) in timing neuronal assembly, plasticity, and maintenance. Caltech Library 2021-09-23 /pmc/articles/PMC9015712/ /pubmed/35622497 http://dx.doi.org/10.17912/micropub.biology.000472 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Shih, Mushaine Chang, Chieh Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL |
title | Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL |
title_full | Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL |
title_fullStr | Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL |
title_full_unstemmed | Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL |
title_short | Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL |
title_sort | brain-wide identification of lin-41 (trim71) protein-expressing neurons by neuropal |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015712/ https://www.ncbi.nlm.nih.gov/pubmed/35622497 http://dx.doi.org/10.17912/micropub.biology.000472 |
work_keys_str_mv | AT shihmushaine brainwideidentificationoflin41trim71proteinexpressingneuronsbyneuropal AT changchieh brainwideidentificationoflin41trim71proteinexpressingneuronsbyneuropal |