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Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL

LIN-41 (TRIM71), an ancient protein best known for its role in timing mitotic stem cell lineages, has been recently shown to be involved in postmitotic neurons to time their differentiation and post-differentiation. Here, we report the identification of 276 LIN-41 protein-expressing neurons in the C...

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Detalles Bibliográficos
Autores principales: Shih, Mushaine, Chang, Chieh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015712/
https://www.ncbi.nlm.nih.gov/pubmed/35622497
http://dx.doi.org/10.17912/micropub.biology.000472
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author Shih, Mushaine
Chang, Chieh
author_facet Shih, Mushaine
Chang, Chieh
author_sort Shih, Mushaine
collection PubMed
description LIN-41 (TRIM71), an ancient protein best known for its role in timing mitotic stem cell lineages, has been recently shown to be involved in postmitotic neurons to time their differentiation and post-differentiation. Here, we report the identification of 276 LIN-41 protein-expressing neurons in the C. elegans nervous system by NeuroPAL and a CRISPR-engineered mNG::LIN-41 reporter, which represents 91% of all hermaphrodite neurons and includes 87 neurons that were not previously reported by CeNGEN using single-cell RNA-seq. Broad lin-41 protein expression in C. elegans neurons suggests a widespread role of LIN-41 (TRIM71) in timing neuronal assembly, plasticity, and maintenance.
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spelling pubmed-90157122022-04-19 Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL Shih, Mushaine Chang, Chieh MicroPubl Biol New Finding LIN-41 (TRIM71), an ancient protein best known for its role in timing mitotic stem cell lineages, has been recently shown to be involved in postmitotic neurons to time their differentiation and post-differentiation. Here, we report the identification of 276 LIN-41 protein-expressing neurons in the C. elegans nervous system by NeuroPAL and a CRISPR-engineered mNG::LIN-41 reporter, which represents 91% of all hermaphrodite neurons and includes 87 neurons that were not previously reported by CeNGEN using single-cell RNA-seq. Broad lin-41 protein expression in C. elegans neurons suggests a widespread role of LIN-41 (TRIM71) in timing neuronal assembly, plasticity, and maintenance. Caltech Library 2021-09-23 /pmc/articles/PMC9015712/ /pubmed/35622497 http://dx.doi.org/10.17912/micropub.biology.000472 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Shih, Mushaine
Chang, Chieh
Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL
title Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL
title_full Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL
title_fullStr Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL
title_full_unstemmed Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL
title_short Brain-wide identification of LIN-41 (TRIM71) protein-expressing neurons by NeuroPAL
title_sort brain-wide identification of lin-41 (trim71) protein-expressing neurons by neuropal
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015712/
https://www.ncbi.nlm.nih.gov/pubmed/35622497
http://dx.doi.org/10.17912/micropub.biology.000472
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