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LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle

The cuticle of C. elegans presents the second largest surface area in the soma of worms. We have recently reported that the permeability barrier in the cuticle is dependent on six permeability-determining (PD) collagens and BLMP-1, a transcription factor. To identify additional regulators of cuticle...

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Autores principales: Sandhu, Anjali, Sheokand, Riya, Singh, Varsha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015713/
https://www.ncbi.nlm.nih.gov/pubmed/35622525
http://dx.doi.org/10.17912/micropub.biology.000434
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author Sandhu, Anjali
Sheokand, Riya
Singh, Varsha
author_facet Sandhu, Anjali
Sheokand, Riya
Singh, Varsha
author_sort Sandhu, Anjali
collection PubMed
description The cuticle of C. elegans presents the second largest surface area in the soma of worms. We have recently reported that the permeability barrier in the cuticle is dependent on six permeability-determining (PD) collagens and BLMP-1, a transcription factor. To identify additional regulators of cuticle permeability, we performed RNA interference for 286 transcription factors expressed in the C. elegans hypodermis and studied cuticle permeability to Hoechst 33258, a nucleic acid dye. Although the cuticle of wild type N2 strain is impermeable to this dye, RNAi of lir-1 or T26A8.1 (PDTF-1) caused permeability defect in the cuticle. LIR- 1 and PDTF-1 positively regulate expression of collagen and/or collagen processing enzymes. As a consequence, lir-1 and pdtf-1 RNAi also caused enhanced susceptibility to exogenous toxins such as paraquat, levamisole and ivermectin. Thus, LIR-1 and PDTF-1 are two hypodermis-specific transcriptional regulators of cuticle permeability.
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spelling pubmed-90157132022-04-19 LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle Sandhu, Anjali Sheokand, Riya Singh, Varsha MicroPubl Biol New Finding The cuticle of C. elegans presents the second largest surface area in the soma of worms. We have recently reported that the permeability barrier in the cuticle is dependent on six permeability-determining (PD) collagens and BLMP-1, a transcription factor. To identify additional regulators of cuticle permeability, we performed RNA interference for 286 transcription factors expressed in the C. elegans hypodermis and studied cuticle permeability to Hoechst 33258, a nucleic acid dye. Although the cuticle of wild type N2 strain is impermeable to this dye, RNAi of lir-1 or T26A8.1 (PDTF-1) caused permeability defect in the cuticle. LIR- 1 and PDTF-1 positively regulate expression of collagen and/or collagen processing enzymes. As a consequence, lir-1 and pdtf-1 RNAi also caused enhanced susceptibility to exogenous toxins such as paraquat, levamisole and ivermectin. Thus, LIR-1 and PDTF-1 are two hypodermis-specific transcriptional regulators of cuticle permeability. Caltech Library 2021-07-27 /pmc/articles/PMC9015713/ /pubmed/35622525 http://dx.doi.org/10.17912/micropub.biology.000434 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Sandhu, Anjali
Sheokand, Riya
Singh, Varsha
LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle
title LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle
title_full LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle
title_fullStr LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle
title_full_unstemmed LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle
title_short LIR-1 and PDTF-1 regulate the permeability barrier function of the C. elegans cuticle
title_sort lir-1 and pdtf-1 regulate the permeability barrier function of the c. elegans cuticle
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015713/
https://www.ncbi.nlm.nih.gov/pubmed/35622525
http://dx.doi.org/10.17912/micropub.biology.000434
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