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Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum e...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015872/ https://www.ncbi.nlm.nih.gov/pubmed/35445134 http://dx.doi.org/10.1155/2022/3811036 |
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author | Chen, Fujia Xu, Yilin Ding, Nana Li, Hongyan Li, Tongbiao Liu, Fengyun Liang, Mengxue Song, Li Liu, Junhe Li, Enzhong Liu, Jiayang |
author_facet | Chen, Fujia Xu, Yilin Ding, Nana Li, Hongyan Li, Tongbiao Liu, Fengyun Liang, Mengxue Song, Li Liu, Junhe Li, Enzhong Liu, Jiayang |
author_sort | Chen, Fujia |
collection | PubMed |
description | This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase—1.0%, papain—1.0%, pectase—0.5%, pH—5, extraction temperature—50°C, and liquid-to-solid ratio—30 mL/g; prediction value of RTPs was 7.54%; the experimental yield of RTPs was 7.22%, while 50 minutes was optimized in Weibull kinetics model. Then high-dose groups of RTP extract could reduce the TC, TG, and LDL-C levels and increase the level of HDL-C in high-fat mice, with the ability to lower the MDA content and enhance SOD level. |
format | Online Article Text |
id | pubmed-9015872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-90158722022-04-19 Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application Chen, Fujia Xu, Yilin Ding, Nana Li, Hongyan Li, Tongbiao Liu, Fengyun Liang, Mengxue Song, Li Liu, Junhe Li, Enzhong Liu, Jiayang Biomed Res Int Research Article This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase—1.0%, papain—1.0%, pectase—0.5%, pH—5, extraction temperature—50°C, and liquid-to-solid ratio—30 mL/g; prediction value of RTPs was 7.54%; the experimental yield of RTPs was 7.22%, while 50 minutes was optimized in Weibull kinetics model. Then high-dose groups of RTP extract could reduce the TC, TG, and LDL-C levels and increase the level of HDL-C in high-fat mice, with the ability to lower the MDA content and enhance SOD level. Hindawi 2022-04-11 /pmc/articles/PMC9015872/ /pubmed/35445134 http://dx.doi.org/10.1155/2022/3811036 Text en Copyright © 2022 Fujia Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Fujia Xu, Yilin Ding, Nana Li, Hongyan Li, Tongbiao Liu, Fengyun Liang, Mengxue Song, Li Liu, Junhe Li, Enzhong Liu, Jiayang Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application |
title | Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application |
title_full | Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application |
title_fullStr | Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application |
title_full_unstemmed | Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application |
title_short | Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application |
title_sort | extraction of radix trichosanthis polysaccharides for potential antihyperlipidemic application |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015872/ https://www.ncbi.nlm.nih.gov/pubmed/35445134 http://dx.doi.org/10.1155/2022/3811036 |
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