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Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application

This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum e...

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Autores principales: Chen, Fujia, Xu, Yilin, Ding, Nana, Li, Hongyan, Li, Tongbiao, Liu, Fengyun, Liang, Mengxue, Song, Li, Liu, Junhe, Li, Enzhong, Liu, Jiayang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015872/
https://www.ncbi.nlm.nih.gov/pubmed/35445134
http://dx.doi.org/10.1155/2022/3811036
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author Chen, Fujia
Xu, Yilin
Ding, Nana
Li, Hongyan
Li, Tongbiao
Liu, Fengyun
Liang, Mengxue
Song, Li
Liu, Junhe
Li, Enzhong
Liu, Jiayang
author_facet Chen, Fujia
Xu, Yilin
Ding, Nana
Li, Hongyan
Li, Tongbiao
Liu, Fengyun
Liang, Mengxue
Song, Li
Liu, Junhe
Li, Enzhong
Liu, Jiayang
author_sort Chen, Fujia
collection PubMed
description This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase—1.0%, papain—1.0%, pectase—0.5%, pH—5, extraction temperature—50°C, and liquid-to-solid ratio—30 mL/g; prediction value of RTPs was 7.54%; the experimental yield of RTPs was 7.22%, while 50 minutes was optimized in Weibull kinetics model. Then high-dose groups of RTP extract could reduce the TC, TG, and LDL-C levels and increase the level of HDL-C in high-fat mice, with the ability to lower the MDA content and enhance SOD level.
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spelling pubmed-90158722022-04-19 Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application Chen, Fujia Xu, Yilin Ding, Nana Li, Hongyan Li, Tongbiao Liu, Fengyun Liang, Mengxue Song, Li Liu, Junhe Li, Enzhong Liu, Jiayang Biomed Res Int Research Article This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase—1.0%, papain—1.0%, pectase—0.5%, pH—5, extraction temperature—50°C, and liquid-to-solid ratio—30 mL/g; prediction value of RTPs was 7.54%; the experimental yield of RTPs was 7.22%, while 50 minutes was optimized in Weibull kinetics model. Then high-dose groups of RTP extract could reduce the TC, TG, and LDL-C levels and increase the level of HDL-C in high-fat mice, with the ability to lower the MDA content and enhance SOD level. Hindawi 2022-04-11 /pmc/articles/PMC9015872/ /pubmed/35445134 http://dx.doi.org/10.1155/2022/3811036 Text en Copyright © 2022 Fujia Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Fujia
Xu, Yilin
Ding, Nana
Li, Hongyan
Li, Tongbiao
Liu, Fengyun
Liang, Mengxue
Song, Li
Liu, Junhe
Li, Enzhong
Liu, Jiayang
Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
title Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
title_full Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
title_fullStr Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
title_full_unstemmed Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
title_short Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
title_sort extraction of radix trichosanthis polysaccharides for potential antihyperlipidemic application
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015872/
https://www.ncbi.nlm.nih.gov/pubmed/35445134
http://dx.doi.org/10.1155/2022/3811036
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