Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients

Viral infections and reactivations are major causes of morbidity and mortality after hematopoietic stem cell (HSCT) and solid organ transplantation (SOT) as well as in patients with immunodeficiencies. Latent herpesviruses (e.g., cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6), lytic v...

Descripción completa

Detalles Bibliográficos
Autores principales: Schulze Lammers, Friederike C., Bonifacius, Agnes, Tischer-Zimmermann, Sabine, Goudeva, Lilia, Martens, Jörg, Lepenies, Bernd, von Karpowitz, Maria, Einecke, Gunilla, Beutel, Gernot, Skripuletz, Thomas, Blasczyk, Rainer, Beier, Rita, Maecker-Kolhoff, Britta, Eiz-Vesper, Britta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015970/
https://www.ncbi.nlm.nih.gov/pubmed/34989946
http://dx.doi.org/10.1007/s10875-021-01205-1
_version_ 1784688427613028352
author Schulze Lammers, Friederike C.
Bonifacius, Agnes
Tischer-Zimmermann, Sabine
Goudeva, Lilia
Martens, Jörg
Lepenies, Bernd
von Karpowitz, Maria
Einecke, Gunilla
Beutel, Gernot
Skripuletz, Thomas
Blasczyk, Rainer
Beier, Rita
Maecker-Kolhoff, Britta
Eiz-Vesper, Britta
author_facet Schulze Lammers, Friederike C.
Bonifacius, Agnes
Tischer-Zimmermann, Sabine
Goudeva, Lilia
Martens, Jörg
Lepenies, Bernd
von Karpowitz, Maria
Einecke, Gunilla
Beutel, Gernot
Skripuletz, Thomas
Blasczyk, Rainer
Beier, Rita
Maecker-Kolhoff, Britta
Eiz-Vesper, Britta
author_sort Schulze Lammers, Friederike C.
collection PubMed
description Viral infections and reactivations are major causes of morbidity and mortality after hematopoietic stem cell (HSCT) and solid organ transplantation (SOT) as well as in patients with immunodeficiencies. Latent herpesviruses (e.g., cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6), lytic viruses (e.g., adenovirus), and polyomaviruses (e.g., BK virus, JC virus) can cause severe complications. Antiviral drugs form the mainstay of treatment for viral infections and reactivations after transplantation, but they have side effects and cannot achieve complete viral clearance without prior reconstitution of functional antiviral T-cell immunity. The aim of this study was to establish normal ranges for virus-specific T-cell (VST) frequencies in healthy donors. Such data are needed for better interpretation of VST frequencies observed in immunocompromised patients. Therefore, we measured the frequencies of VSTs against 23 viral protein-derived peptide pools from 11 clinically relevant human viruses in blood from healthy donors (n = 151). Specifically, we determined the VST frequencies by interferon-gamma enzyme-linked immunospot assay and classified their distribution according to age and gender to allow for a more specific evaluation and prediction of antiviral immune responses. The reference values established here provide an invaluable tool for immune response evaluation, intensity of therapeutic drugs and treatment decision-making in immunosuppressed patients. This data should make an important contribution to improving the assessment of immune responses in immunocompromised patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10875-021-01205-1.
format Online
Article
Text
id pubmed-9015970
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-90159702022-05-02 Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients Schulze Lammers, Friederike C. Bonifacius, Agnes Tischer-Zimmermann, Sabine Goudeva, Lilia Martens, Jörg Lepenies, Bernd von Karpowitz, Maria Einecke, Gunilla Beutel, Gernot Skripuletz, Thomas Blasczyk, Rainer Beier, Rita Maecker-Kolhoff, Britta Eiz-Vesper, Britta J Clin Immunol Original Article Viral infections and reactivations are major causes of morbidity and mortality after hematopoietic stem cell (HSCT) and solid organ transplantation (SOT) as well as in patients with immunodeficiencies. Latent herpesviruses (e.g., cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6), lytic viruses (e.g., adenovirus), and polyomaviruses (e.g., BK virus, JC virus) can cause severe complications. Antiviral drugs form the mainstay of treatment for viral infections and reactivations after transplantation, but they have side effects and cannot achieve complete viral clearance without prior reconstitution of functional antiviral T-cell immunity. The aim of this study was to establish normal ranges for virus-specific T-cell (VST) frequencies in healthy donors. Such data are needed for better interpretation of VST frequencies observed in immunocompromised patients. Therefore, we measured the frequencies of VSTs against 23 viral protein-derived peptide pools from 11 clinically relevant human viruses in blood from healthy donors (n = 151). Specifically, we determined the VST frequencies by interferon-gamma enzyme-linked immunospot assay and classified their distribution according to age and gender to allow for a more specific evaluation and prediction of antiviral immune responses. The reference values established here provide an invaluable tool for immune response evaluation, intensity of therapeutic drugs and treatment decision-making in immunosuppressed patients. This data should make an important contribution to improving the assessment of immune responses in immunocompromised patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10875-021-01205-1. Springer US 2022-01-06 2022 /pmc/articles/PMC9015970/ /pubmed/34989946 http://dx.doi.org/10.1007/s10875-021-01205-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Schulze Lammers, Friederike C.
Bonifacius, Agnes
Tischer-Zimmermann, Sabine
Goudeva, Lilia
Martens, Jörg
Lepenies, Bernd
von Karpowitz, Maria
Einecke, Gunilla
Beutel, Gernot
Skripuletz, Thomas
Blasczyk, Rainer
Beier, Rita
Maecker-Kolhoff, Britta
Eiz-Vesper, Britta
Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients
title Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients
title_full Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients
title_fullStr Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients
title_full_unstemmed Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients
title_short Antiviral T-Cell Frequencies in a Healthy Population: Reference Values for Evaluating Antiviral Immune Cell Profiles in Immunocompromised Patients
title_sort antiviral t-cell frequencies in a healthy population: reference values for evaluating antiviral immune cell profiles in immunocompromised patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9015970/
https://www.ncbi.nlm.nih.gov/pubmed/34989946
http://dx.doi.org/10.1007/s10875-021-01205-1
work_keys_str_mv AT schulzelammersfriederikec antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT bonifaciusagnes antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT tischerzimmermannsabine antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT goudevalilia antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT martensjorg antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT lepeniesbernd antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT vonkarpowitzmaria antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT eineckegunilla antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT beutelgernot antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT skripuletzthomas antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT blasczykrainer antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT beierrita antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT maeckerkolhoffbritta antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients
AT eizvesperbritta antiviraltcellfrequenciesinahealthypopulationreferencevaluesforevaluatingantiviralimmunecellprofilesinimmunocompromisedpatients