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Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature

Electron microscopy is the primary approach to study ultrastructural features of the cerebrovasculature. However, 2D snapshots of a vascular bed capture only a small fraction of its complexity. Recent efforts to synaptically map neuronal circuitry using volume electron microscopy have also sampled t...

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Autores principales: Bonney, Stephanie K., Coelho-Santos, Vanessa, Huang, Sheng-Fu, Takeno, Marc, Kornfeld, Joergen, Keller, Annika, Shih, Andy Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016339/
https://www.ncbi.nlm.nih.gov/pubmed/35450291
http://dx.doi.org/10.3389/fcell.2022.849469
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author Bonney, Stephanie K.
Coelho-Santos, Vanessa
Huang, Sheng-Fu
Takeno, Marc
Kornfeld, Joergen
Keller, Annika
Shih, Andy Y.
author_facet Bonney, Stephanie K.
Coelho-Santos, Vanessa
Huang, Sheng-Fu
Takeno, Marc
Kornfeld, Joergen
Keller, Annika
Shih, Andy Y.
author_sort Bonney, Stephanie K.
collection PubMed
description Electron microscopy is the primary approach to study ultrastructural features of the cerebrovasculature. However, 2D snapshots of a vascular bed capture only a small fraction of its complexity. Recent efforts to synaptically map neuronal circuitry using volume electron microscopy have also sampled the brain microvasculature in 3D. Here, we perform a meta-analysis of 7 data sets spanning different species and brain regions, including two data sets from the MICrONS consortium that have made efforts to segment vasculature in addition to all parenchymal cell types in mouse visual cortex. Exploration of these data have revealed rich information for detailed investigation of the cerebrovasculature. Neurovascular unit cell types (including, but not limited to, endothelial cells, mural cells, perivascular fibroblasts, microglia, and astrocytes) could be discerned across broad microvascular zones. Image contrast was sufficient to identify subcellular details, including endothelial junctions, caveolae, peg-and-socket interactions, mitochondria, Golgi cisternae, microvilli and other cellular protrusions of potential significance to vascular signaling. Additionally, non-cellular structures including the basement membrane and perivascular spaces were visible and could be traced between arterio-venous zones along the vascular wall. These explorations revealed structural features that may be important for vascular functions, such as blood-brain barrier integrity, blood flow control, brain clearance, and bioenergetics. They also identified limitations where accuracy and consistency of segmentation could be further honed by future efforts. The purpose of this article is to introduce these valuable community resources within the framework of cerebrovascular research. We do so by providing an assessment of their vascular contents, identifying features of significance for further study, and discussing next step ideas for refining vascular segmentation and analysis.
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spelling pubmed-90163392022-04-20 Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature Bonney, Stephanie K. Coelho-Santos, Vanessa Huang, Sheng-Fu Takeno, Marc Kornfeld, Joergen Keller, Annika Shih, Andy Y. Front Cell Dev Biol Cell and Developmental Biology Electron microscopy is the primary approach to study ultrastructural features of the cerebrovasculature. However, 2D snapshots of a vascular bed capture only a small fraction of its complexity. Recent efforts to synaptically map neuronal circuitry using volume electron microscopy have also sampled the brain microvasculature in 3D. Here, we perform a meta-analysis of 7 data sets spanning different species and brain regions, including two data sets from the MICrONS consortium that have made efforts to segment vasculature in addition to all parenchymal cell types in mouse visual cortex. Exploration of these data have revealed rich information for detailed investigation of the cerebrovasculature. Neurovascular unit cell types (including, but not limited to, endothelial cells, mural cells, perivascular fibroblasts, microglia, and astrocytes) could be discerned across broad microvascular zones. Image contrast was sufficient to identify subcellular details, including endothelial junctions, caveolae, peg-and-socket interactions, mitochondria, Golgi cisternae, microvilli and other cellular protrusions of potential significance to vascular signaling. Additionally, non-cellular structures including the basement membrane and perivascular spaces were visible and could be traced between arterio-venous zones along the vascular wall. These explorations revealed structural features that may be important for vascular functions, such as blood-brain barrier integrity, blood flow control, brain clearance, and bioenergetics. They also identified limitations where accuracy and consistency of segmentation could be further honed by future efforts. The purpose of this article is to introduce these valuable community resources within the framework of cerebrovascular research. We do so by providing an assessment of their vascular contents, identifying features of significance for further study, and discussing next step ideas for refining vascular segmentation and analysis. Frontiers Media S.A. 2022-04-05 /pmc/articles/PMC9016339/ /pubmed/35450291 http://dx.doi.org/10.3389/fcell.2022.849469 Text en Copyright © 2022 Bonney, Coelho-Santos, Huang, Takeno, Kornfeld, Keller and Shih. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Bonney, Stephanie K.
Coelho-Santos, Vanessa
Huang, Sheng-Fu
Takeno, Marc
Kornfeld, Joergen
Keller, Annika
Shih, Andy Y.
Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature
title Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature
title_full Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature
title_fullStr Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature
title_full_unstemmed Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature
title_short Public Volume Electron Microscopy Data: An Essential Resource to Study the Brain Microvasculature
title_sort public volume electron microscopy data: an essential resource to study the brain microvasculature
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016339/
https://www.ncbi.nlm.nih.gov/pubmed/35450291
http://dx.doi.org/10.3389/fcell.2022.849469
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