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Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants

Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, and squash, contribute greatly to the human diet. The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement. However, most economically import...

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Autores principales: Xin, Tongxu, Tian, Haojie, Ma, Yalin, Wang, Shenhao, Yang, Li, Li, Xutong, Zhang, Mengzhuo, Chen, Chen, Wang, Huaisong, Li, Haizhen, Xu, Jieting, Huang, Sanwen, Yang, Xueyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016859/
https://www.ncbi.nlm.nih.gov/pubmed/35048122
http://dx.doi.org/10.1093/hr/uhab086
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author Xin, Tongxu
Tian, Haojie
Ma, Yalin
Wang, Shenhao
Yang, Li
Li, Xutong
Zhang, Mengzhuo
Chen, Chen
Wang, Huaisong
Li, Haizhen
Xu, Jieting
Huang, Sanwen
Yang, Xueyong
author_facet Xin, Tongxu
Tian, Haojie
Ma, Yalin
Wang, Shenhao
Yang, Li
Li, Xutong
Zhang, Mengzhuo
Chen, Chen
Wang, Huaisong
Li, Haizhen
Xu, Jieting
Huang, Sanwen
Yang, Xueyong
author_sort Xin, Tongxu
collection PubMed
description Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, and squash, contribute greatly to the human diet. The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement. However, most economically important cucurbit plants, including melon and squash, remain recalcitrant to standard Agrobacterium tumefaciens-mediated transformation, limiting the effective use of genome editing technology. In this study, we used an “optimal infiltration intensity” strategy to establish an efficient genetic transformation system for melon and squash. We harnessed the power of this method to target homologs of the ERECTA family of receptor kinase genes and created alleles that resulted in a compact plant architecture with shorter internodes in melon, squash, and cucumber. The optimized transformation method presented here enables stable CRISPR/Cas9-mediated mutagenesis and provides a solid foundation for functional gene manipulation in cucurbit crops.
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spelling pubmed-90168592022-04-20 Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants Xin, Tongxu Tian, Haojie Ma, Yalin Wang, Shenhao Yang, Li Li, Xutong Zhang, Mengzhuo Chen, Chen Wang, Huaisong Li, Haizhen Xu, Jieting Huang, Sanwen Yang, Xueyong Hortic Res Article Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, and squash, contribute greatly to the human diet. The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement. However, most economically important cucurbit plants, including melon and squash, remain recalcitrant to standard Agrobacterium tumefaciens-mediated transformation, limiting the effective use of genome editing technology. In this study, we used an “optimal infiltration intensity” strategy to establish an efficient genetic transformation system for melon and squash. We harnessed the power of this method to target homologs of the ERECTA family of receptor kinase genes and created alleles that resulted in a compact plant architecture with shorter internodes in melon, squash, and cucumber. The optimized transformation method presented here enables stable CRISPR/Cas9-mediated mutagenesis and provides a solid foundation for functional gene manipulation in cucurbit crops. Oxford University Press 2022-01-20 /pmc/articles/PMC9016859/ /pubmed/35048122 http://dx.doi.org/10.1093/hr/uhab086 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Xin, Tongxu
Tian, Haojie
Ma, Yalin
Wang, Shenhao
Yang, Li
Li, Xutong
Zhang, Mengzhuo
Chen, Chen
Wang, Huaisong
Li, Haizhen
Xu, Jieting
Huang, Sanwen
Yang, Xueyong
Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
title Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
title_full Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
title_fullStr Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
title_full_unstemmed Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
title_short Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
title_sort targeted creation of new mutants with compact plant architecture using crispr/cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016859/
https://www.ncbi.nlm.nih.gov/pubmed/35048122
http://dx.doi.org/10.1093/hr/uhab086
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