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Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, and squash, contribute greatly to the human diet. The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement. However, most economically import...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016859/ https://www.ncbi.nlm.nih.gov/pubmed/35048122 http://dx.doi.org/10.1093/hr/uhab086 |
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author | Xin, Tongxu Tian, Haojie Ma, Yalin Wang, Shenhao Yang, Li Li, Xutong Zhang, Mengzhuo Chen, Chen Wang, Huaisong Li, Haizhen Xu, Jieting Huang, Sanwen Yang, Xueyong |
author_facet | Xin, Tongxu Tian, Haojie Ma, Yalin Wang, Shenhao Yang, Li Li, Xutong Zhang, Mengzhuo Chen, Chen Wang, Huaisong Li, Haizhen Xu, Jieting Huang, Sanwen Yang, Xueyong |
author_sort | Xin, Tongxu |
collection | PubMed |
description | Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, and squash, contribute greatly to the human diet. The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement. However, most economically important cucurbit plants, including melon and squash, remain recalcitrant to standard Agrobacterium tumefaciens-mediated transformation, limiting the effective use of genome editing technology. In this study, we used an “optimal infiltration intensity” strategy to establish an efficient genetic transformation system for melon and squash. We harnessed the power of this method to target homologs of the ERECTA family of receptor kinase genes and created alleles that resulted in a compact plant architecture with shorter internodes in melon, squash, and cucumber. The optimized transformation method presented here enables stable CRISPR/Cas9-mediated mutagenesis and provides a solid foundation for functional gene manipulation in cucurbit crops. |
format | Online Article Text |
id | pubmed-9016859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-90168592022-04-20 Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants Xin, Tongxu Tian, Haojie Ma, Yalin Wang, Shenhao Yang, Li Li, Xutong Zhang, Mengzhuo Chen, Chen Wang, Huaisong Li, Haizhen Xu, Jieting Huang, Sanwen Yang, Xueyong Hortic Res Article Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, and squash, contribute greatly to the human diet. The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement. However, most economically important cucurbit plants, including melon and squash, remain recalcitrant to standard Agrobacterium tumefaciens-mediated transformation, limiting the effective use of genome editing technology. In this study, we used an “optimal infiltration intensity” strategy to establish an efficient genetic transformation system for melon and squash. We harnessed the power of this method to target homologs of the ERECTA family of receptor kinase genes and created alleles that resulted in a compact plant architecture with shorter internodes in melon, squash, and cucumber. The optimized transformation method presented here enables stable CRISPR/Cas9-mediated mutagenesis and provides a solid foundation for functional gene manipulation in cucurbit crops. Oxford University Press 2022-01-20 /pmc/articles/PMC9016859/ /pubmed/35048122 http://dx.doi.org/10.1093/hr/uhab086 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Xin, Tongxu Tian, Haojie Ma, Yalin Wang, Shenhao Yang, Li Li, Xutong Zhang, Mengzhuo Chen, Chen Wang, Huaisong Li, Haizhen Xu, Jieting Huang, Sanwen Yang, Xueyong Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants |
title | Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants |
title_full | Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants |
title_fullStr | Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants |
title_full_unstemmed | Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants |
title_short | Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants |
title_sort | targeted creation of new mutants with compact plant architecture using crispr/cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016859/ https://www.ncbi.nlm.nih.gov/pubmed/35048122 http://dx.doi.org/10.1093/hr/uhab086 |
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