Cargando…
Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation
BACKGROUND: Lots of studies have measured motor evoked potential (MEP) induced by transcranial magnetic stimulation (TMS) in anesthetized animals. However, in awake animals, the measurement of TMS-induced MEP is scarce as lack of sufficient restraint. So far, the explicit study of anesthesia effects...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016971/ https://www.ncbi.nlm.nih.gov/pubmed/35439927 http://dx.doi.org/10.1186/s12871-022-01655-z |
_version_ | 1784688672623296512 |
---|---|
author | Wang, Xin Wang, Tengfei Jin, Jingna Wang, He Li, Ying Liu, Zhipeng Yin, Tao |
author_facet | Wang, Xin Wang, Tengfei Jin, Jingna Wang, He Li, Ying Liu, Zhipeng Yin, Tao |
author_sort | Wang, Xin |
collection | PubMed |
description | BACKGROUND: Lots of studies have measured motor evoked potential (MEP) induced by transcranial magnetic stimulation (TMS) in anesthetized animals. However, in awake animals, the measurement of TMS-induced MEP is scarce as lack of sufficient restraint. So far, the explicit study of anesthesia effects on corticospinal excitability and repetitive TMS (rTMS) induced modulation is still lacking. This study aimed to: (1) measure TMS-induced MEP in both awake restrained and anesthetized rats, (2) investigate the effect of anesthesia on corticospinal excitability, and (3) on rTMS-induced modulation. METHODS: MEP of eighteen rats were measured under both wakefulness and anesthesia using flexible binding and surface electrodes. Peak-to-peak MEP amplitudes, resting motor threshold (RMT) and the slope of stimulus response (SR) were extracted to investigate anesthesia effects on corticospinal excitability. Thereafter, 5 or 10 Hz rTMS was applied with 600 pulses, and the increase in MEP amplitude and the decrease in RMT were used to quantify rTMS-induced modulation. RESULTS: The RMT in the awake condition was 44.6 ± 1.2% maximum output (MO), the peak-to-peak MEP amplitude was 404.6 ± 48.8 μV at 60% MO. Under anesthesia, higher RMT (55.6 ± 2.9% MO), lower peak-to-peak MEP amplitudes (258.6 ± 32.7 μV) and lower slope of SR indicated that the corticospinal excitability was suppressed. Moreover, under anesthesia, high-frequency rTMS still showed significant modulation of corticospinal excitability, but the modulation of MEP peak-to-peak amplitudes was weaker than that under wakefulness. CONCLUSIONS: This study measured TMS-induced MEP in both awake and anesthetized rats, and provided explicit evidence for the inhibitory effects of anesthesia on corticospinal excitability and on high-frequency rTMS-induced modulation of MEP. |
format | Online Article Text |
id | pubmed-9016971 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90169712022-04-20 Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation Wang, Xin Wang, Tengfei Jin, Jingna Wang, He Li, Ying Liu, Zhipeng Yin, Tao BMC Anesthesiol Research BACKGROUND: Lots of studies have measured motor evoked potential (MEP) induced by transcranial magnetic stimulation (TMS) in anesthetized animals. However, in awake animals, the measurement of TMS-induced MEP is scarce as lack of sufficient restraint. So far, the explicit study of anesthesia effects on corticospinal excitability and repetitive TMS (rTMS) induced modulation is still lacking. This study aimed to: (1) measure TMS-induced MEP in both awake restrained and anesthetized rats, (2) investigate the effect of anesthesia on corticospinal excitability, and (3) on rTMS-induced modulation. METHODS: MEP of eighteen rats were measured under both wakefulness and anesthesia using flexible binding and surface electrodes. Peak-to-peak MEP amplitudes, resting motor threshold (RMT) and the slope of stimulus response (SR) were extracted to investigate anesthesia effects on corticospinal excitability. Thereafter, 5 or 10 Hz rTMS was applied with 600 pulses, and the increase in MEP amplitude and the decrease in RMT were used to quantify rTMS-induced modulation. RESULTS: The RMT in the awake condition was 44.6 ± 1.2% maximum output (MO), the peak-to-peak MEP amplitude was 404.6 ± 48.8 μV at 60% MO. Under anesthesia, higher RMT (55.6 ± 2.9% MO), lower peak-to-peak MEP amplitudes (258.6 ± 32.7 μV) and lower slope of SR indicated that the corticospinal excitability was suppressed. Moreover, under anesthesia, high-frequency rTMS still showed significant modulation of corticospinal excitability, but the modulation of MEP peak-to-peak amplitudes was weaker than that under wakefulness. CONCLUSIONS: This study measured TMS-induced MEP in both awake and anesthetized rats, and provided explicit evidence for the inhibitory effects of anesthesia on corticospinal excitability and on high-frequency rTMS-induced modulation of MEP. BioMed Central 2022-04-19 /pmc/articles/PMC9016971/ /pubmed/35439927 http://dx.doi.org/10.1186/s12871-022-01655-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wang, Xin Wang, Tengfei Jin, Jingna Wang, He Li, Ying Liu, Zhipeng Yin, Tao Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation |
title | Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation |
title_full | Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation |
title_fullStr | Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation |
title_full_unstemmed | Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation |
title_short | Anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation |
title_sort | anesthesia inhibited corticospinal excitability and attenuated the modulation of repetitive transcranial magnetic stimulation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016971/ https://www.ncbi.nlm.nih.gov/pubmed/35439927 http://dx.doi.org/10.1186/s12871-022-01655-z |
work_keys_str_mv | AT wangxin anesthesiainhibitedcorticospinalexcitabilityandattenuatedthemodulationofrepetitivetranscranialmagneticstimulation AT wangtengfei anesthesiainhibitedcorticospinalexcitabilityandattenuatedthemodulationofrepetitivetranscranialmagneticstimulation AT jinjingna anesthesiainhibitedcorticospinalexcitabilityandattenuatedthemodulationofrepetitivetranscranialmagneticstimulation AT wanghe anesthesiainhibitedcorticospinalexcitabilityandattenuatedthemodulationofrepetitivetranscranialmagneticstimulation AT liying anesthesiainhibitedcorticospinalexcitabilityandattenuatedthemodulationofrepetitivetranscranialmagneticstimulation AT liuzhipeng anesthesiainhibitedcorticospinalexcitabilityandattenuatedthemodulationofrepetitivetranscranialmagneticstimulation AT yintao anesthesiainhibitedcorticospinalexcitabilityandattenuatedthemodulationofrepetitivetranscranialmagneticstimulation |