Cargando…

β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge

BACKGROUND: Antimicrobial peptides including various defensins have been attracting considerable research interest worldwide, as they have potential to substitute for antibiotics. Moreover, AMPs also have immunomodulatory activity. In this study, we explored the role and its potential mechanisms of...

Descripción completa

Detalles Bibliográficos
Autores principales: Fu, Qingqing, Lin, Qian, Chen, Daiwen, Yu, Bing, Luo, Yuheng, Zheng, Ping, Mao, Xiangbing, Huang, Zhiqing, Yu, Jie, Luo, Junqiu, Yan, Hui, He, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017018/
https://www.ncbi.nlm.nih.gov/pubmed/35440001
http://dx.doi.org/10.1186/s12917-022-03242-3
_version_ 1784688683754979328
author Fu, Qingqing
Lin, Qian
Chen, Daiwen
Yu, Bing
Luo, Yuheng
Zheng, Ping
Mao, Xiangbing
Huang, Zhiqing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
author_facet Fu, Qingqing
Lin, Qian
Chen, Daiwen
Yu, Bing
Luo, Yuheng
Zheng, Ping
Mao, Xiangbing
Huang, Zhiqing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
author_sort Fu, Qingqing
collection PubMed
description BACKGROUND: Antimicrobial peptides including various defensins have been attracting considerable research interest worldwide, as they have potential to substitute for antibiotics. Moreover, AMPs also have immunomodulatory activity. In this study, we explored the role and its potential mechanisms of β-defensin 118 (DEFB118) in alleviating inflammation and injury of IPEC-J2 cells (porcine jejunum epithelial cell line) upon the enterotoxigenic Escherichia coli (ETEC) challenge. RESULTS: The porcine jejunum epithelial cell line (IPEC-J2) pretreated with or without DEFB118 (25 μg/mL) were challenged by ETEC (1×10(6) CFU) or culture medium. We showed that DEFB118 pretreatment significantly increased the cell viability (P<0.05) and decreased the expressions of inflammatory cytokines such as the interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in IPEC-J2 cells exposure to ETEC (P<0.05). Interestingly, DEFB118 pretreatment significantly elevated the abundance of the major tight-junction protein zonula occludens-1 (ZO-1), but decreased the number of apoptotic cells upon ETEC challenge (P<0.05). The expression of caspase 3, caspase 8, and caspase 9 were downregulated by DEFB118 in the IPEC-J2 cells exposure to ETEC (P<0.05). Importantly, DEFB118 suppressed two critical inflammation-associated signaling proteins, nuclear factor-kappa-B inhibitor alpha (IκB-α) and nuclear factor-kappaB (NF-κB) in the ETEC-challenged IPEC-J2 cells. CONCLUSIONS: DEFB118 can alleviate ETEC-induced inflammation in IPEC-J2 cells through inhibition of the NF-κB signaling pathway, resulting in reduced secretion of inflammatory cytokines and decreased cell apoptosis. Therefore, DEFB118 can act as a novel anti-inflammatory agent.
format Online
Article
Text
id pubmed-9017018
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-90170182022-04-20 β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge Fu, Qingqing Lin, Qian Chen, Daiwen Yu, Bing Luo, Yuheng Zheng, Ping Mao, Xiangbing Huang, Zhiqing Yu, Jie Luo, Junqiu Yan, Hui He, Jun BMC Vet Res Research BACKGROUND: Antimicrobial peptides including various defensins have been attracting considerable research interest worldwide, as they have potential to substitute for antibiotics. Moreover, AMPs also have immunomodulatory activity. In this study, we explored the role and its potential mechanisms of β-defensin 118 (DEFB118) in alleviating inflammation and injury of IPEC-J2 cells (porcine jejunum epithelial cell line) upon the enterotoxigenic Escherichia coli (ETEC) challenge. RESULTS: The porcine jejunum epithelial cell line (IPEC-J2) pretreated with or without DEFB118 (25 μg/mL) were challenged by ETEC (1×10(6) CFU) or culture medium. We showed that DEFB118 pretreatment significantly increased the cell viability (P<0.05) and decreased the expressions of inflammatory cytokines such as the interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in IPEC-J2 cells exposure to ETEC (P<0.05). Interestingly, DEFB118 pretreatment significantly elevated the abundance of the major tight-junction protein zonula occludens-1 (ZO-1), but decreased the number of apoptotic cells upon ETEC challenge (P<0.05). The expression of caspase 3, caspase 8, and caspase 9 were downregulated by DEFB118 in the IPEC-J2 cells exposure to ETEC (P<0.05). Importantly, DEFB118 suppressed two critical inflammation-associated signaling proteins, nuclear factor-kappa-B inhibitor alpha (IκB-α) and nuclear factor-kappaB (NF-κB) in the ETEC-challenged IPEC-J2 cells. CONCLUSIONS: DEFB118 can alleviate ETEC-induced inflammation in IPEC-J2 cells through inhibition of the NF-κB signaling pathway, resulting in reduced secretion of inflammatory cytokines and decreased cell apoptosis. Therefore, DEFB118 can act as a novel anti-inflammatory agent. BioMed Central 2022-04-19 /pmc/articles/PMC9017018/ /pubmed/35440001 http://dx.doi.org/10.1186/s12917-022-03242-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Fu, Qingqing
Lin, Qian
Chen, Daiwen
Yu, Bing
Luo, Yuheng
Zheng, Ping
Mao, Xiangbing
Huang, Zhiqing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge
title β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge
title_full β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge
title_fullStr β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge
title_full_unstemmed β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge
title_short β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge
title_sort β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic escherichia coli challenge
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017018/
https://www.ncbi.nlm.nih.gov/pubmed/35440001
http://dx.doi.org/10.1186/s12917-022-03242-3
work_keys_str_mv AT fuqingqing bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT linqian bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT chendaiwen bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT yubing bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT luoyuheng bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT zhengping bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT maoxiangbing bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT huangzhiqing bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT yujie bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT luojunqiu bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT yanhui bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge
AT hejun bdefensin118attenuatesinflammationandinjuryofintestinalepithelialcellsuponenterotoxigenicescherichiacolichallenge