Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungus Rhizopus microsporus

Mucormycosis is a lethal and emerging disease that has lacked a genetic model fulfilling both high virulence and the possibility of performing stable and reproducible gene manipulation by homologous recombination (HR). Here, we developed a new methodology to successfully perform HR in Rhizopus micro...

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Detalles Bibliográficos
Autores principales: Lax, Carlos, Navarro-Mendoza, María Isabel, Pérez-Arques, Carlos, Navarro, Eusebio, Nicolás, Francisco Esteban, Garre, Victoriano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017206/
https://www.ncbi.nlm.nih.gov/pubmed/35475217
http://dx.doi.org/10.1016/j.crmeth.2021.100124

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017206/
https://www.ncbi.nlm.nih.gov/pubmed/35475217
http://dx.doi.org/10.1016/j.crmeth.2021.100124

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