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Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungus Rhizopus microsporus
Mucormycosis is a lethal and emerging disease that has lacked a genetic model fulfilling both high virulence and the possibility of performing stable and reproducible gene manipulation by homologous recombination (HR). Here, we developed a new methodology to successfully perform HR in Rhizopus micro...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017206/ https://www.ncbi.nlm.nih.gov/pubmed/35475217 http://dx.doi.org/10.1016/j.crmeth.2021.100124 |