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A stress-reduced passaging technique improves the viability of human pluripotent cells

Xeno-free culture systems have expanded the clinical and industrial application of human pluripotent stem cells (PSCs). However, reproducibility issues, often arising from variability during passaging steps, remain. Here, we describe an improved method for the subculture of human PSCs. The revised m...

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Detalles Bibliográficos
Autores principales: Takahashi, Kazutoshi, Okubo, Chikako, Nakamura, Michiko, Iwasaki, Mio, Kawahara, Yuka, Tabata, Tsuyoshi, Miyamoto, Yousuke, Woltjen, Knut, Yamanaka, Shinya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017214/
https://www.ncbi.nlm.nih.gov/pubmed/35474962
http://dx.doi.org/10.1016/j.crmeth.2021.100155