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A stress-reduced passaging technique improves the viability of human pluripotent cells
Xeno-free culture systems have expanded the clinical and industrial application of human pluripotent stem cells (PSCs). However, reproducibility issues, often arising from variability during passaging steps, remain. Here, we describe an improved method for the subculture of human PSCs. The revised m...
Autores principales: | Takahashi, Kazutoshi, Okubo, Chikako, Nakamura, Michiko, Iwasaki, Mio, Kawahara, Yuka, Tabata, Tsuyoshi, Miyamoto, Yousuke, Woltjen, Knut, Yamanaka, Shinya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017214/ https://www.ncbi.nlm.nih.gov/pubmed/35474962 http://dx.doi.org/10.1016/j.crmeth.2021.100155 |
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