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Improving Thermostability of Chimeric Enzymes Generated by Domain Shuffling Between Two Different Original Glucoamylases

In order to improve enzymatic properties of glucoamylases, six recombinant genes GA1–GA6 were created by domain shuffling of glucoamylase genes GAA1 from Aspergillus niger Ld418AI and GATE from Talaromyces emersonii Ld418 TE using overlap extension PCR and were expressed in Saccharomyces cerevisiae...

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Detalles Bibliográficos
Autores principales:	Chen, Zhongxiu, Wang, Longbin, Shen, Yuyu, Hu, Dunji, Zhou, Liying, Lu, Fuping, Li, Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017332/ https://www.ncbi.nlm.nih.gov/pubmed/35449593 http://dx.doi.org/10.3389/fbioe.2022.881421

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