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Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway

Mesenchymal stem cells (MSCs) are multipotent cells that can skew the balance of M1/M2 macrophage polarization towards the M2 phenotype via their paracrine effects, thereby promoting anatomical and functional recovery after many inflammatory diseases induced by macrophages. However, the underlying m...

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Autores principales: Pang, Qi-Ming, Yang, Rui, Zhang, Meng, Zou, Wang-Hui, Qian, Nan-Nan, Xu, Qi-Jing, Chen, Hui, Peng, Jia-Chen, Luo, Xiao-Ping, Zhang, Qian, Zhang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017453/
https://www.ncbi.nlm.nih.gov/pubmed/35450344
http://dx.doi.org/10.1155/2022/5181241
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author Pang, Qi-Ming
Yang, Rui
Zhang, Meng
Zou, Wang-Hui
Qian, Nan-Nan
Xu, Qi-Jing
Chen, Hui
Peng, Jia-Chen
Luo, Xiao-Ping
Zhang, Qian
Zhang, Tao
author_facet Pang, Qi-Ming
Yang, Rui
Zhang, Meng
Zou, Wang-Hui
Qian, Nan-Nan
Xu, Qi-Jing
Chen, Hui
Peng, Jia-Chen
Luo, Xiao-Ping
Zhang, Qian
Zhang, Tao
author_sort Pang, Qi-Ming
collection PubMed
description Mesenchymal stem cells (MSCs) are multipotent cells that can skew the balance of M1/M2 macrophage polarization towards the M2 phenotype via their paracrine effects, thereby promoting anatomical and functional recovery after many inflammatory diseases induced by macrophages. However, the underlying mechanism is still poorly understood. This study focused on the IL-10/STAT3 pathway and investigated whether IL-10 secreted by PBMSCs could mediate M2 polarization through the activation of this pathway. In this study, a Transwell system was used for coculturing macrophages and PBMSCs. ELISA and RT-qPCR analysis found that PBMSCs and their conditioned media (P-CM) significantly induced the expression of IL-10, while significantly inhibiting the expression of IL-1β and TNF-α; moreover, this effect could be reversed by adding Ab9969 (an IL-10 neutralizing antibody) and Stattic (a STAT3 inhibitor). Furthermore, western blotting and immunofluorescence assays demonstrated that JAK1/STAT3 signaling was significantly upregulated in macrophages cocultured with PBMSCs or P-CM, accompanied by an increase in the M2 biomarker CD206 and a decrease in the M1 biomarker CD86. This effect could also be reversed by blocking the IL-10/STAT3 pathway with Ab9969 and Stattic. In summary, PBMSCs could mediate the polarization of M2 macrophages by activating the IL-10/STAT3 pathway.
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spelling pubmed-90174532022-04-20 Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway Pang, Qi-Ming Yang, Rui Zhang, Meng Zou, Wang-Hui Qian, Nan-Nan Xu, Qi-Jing Chen, Hui Peng, Jia-Chen Luo, Xiao-Ping Zhang, Qian Zhang, Tao Stem Cells Int Research Article Mesenchymal stem cells (MSCs) are multipotent cells that can skew the balance of M1/M2 macrophage polarization towards the M2 phenotype via their paracrine effects, thereby promoting anatomical and functional recovery after many inflammatory diseases induced by macrophages. However, the underlying mechanism is still poorly understood. This study focused on the IL-10/STAT3 pathway and investigated whether IL-10 secreted by PBMSCs could mediate M2 polarization through the activation of this pathway. In this study, a Transwell system was used for coculturing macrophages and PBMSCs. ELISA and RT-qPCR analysis found that PBMSCs and their conditioned media (P-CM) significantly induced the expression of IL-10, while significantly inhibiting the expression of IL-1β and TNF-α; moreover, this effect could be reversed by adding Ab9969 (an IL-10 neutralizing antibody) and Stattic (a STAT3 inhibitor). Furthermore, western blotting and immunofluorescence assays demonstrated that JAK1/STAT3 signaling was significantly upregulated in macrophages cocultured with PBMSCs or P-CM, accompanied by an increase in the M2 biomarker CD206 and a decrease in the M1 biomarker CD86. This effect could also be reversed by blocking the IL-10/STAT3 pathway with Ab9969 and Stattic. In summary, PBMSCs could mediate the polarization of M2 macrophages by activating the IL-10/STAT3 pathway. Hindawi 2022-04-11 /pmc/articles/PMC9017453/ /pubmed/35450344 http://dx.doi.org/10.1155/2022/5181241 Text en Copyright © 2022 Qi-Ming Pang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pang, Qi-Ming
Yang, Rui
Zhang, Meng
Zou, Wang-Hui
Qian, Nan-Nan
Xu, Qi-Jing
Chen, Hui
Peng, Jia-Chen
Luo, Xiao-Ping
Zhang, Qian
Zhang, Tao
Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway
title Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway
title_full Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway
title_fullStr Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway
title_full_unstemmed Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway
title_short Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway
title_sort peripheral blood-derived mesenchymal stem cells modulate macrophage plasticity through the il-10/stat3 pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017453/
https://www.ncbi.nlm.nih.gov/pubmed/35450344
http://dx.doi.org/10.1155/2022/5181241
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