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Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential

Mesenchymal stem cells (MSCs) are effective therapeutic agents that contribute to tissue repair and regeneration by secreting various factors. However, donor-dependent variations in MSC proliferation and therapeutic potentials result in variable production yields and clinical outcomes, thereby imped...

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Autores principales: Kim, Sun Jeong, Park, Sang Eon, Jeong, Jang Bin, Oh, Shin Ji, Choi, Alee, Kim, Yun Hee, Choi, Suk-joo, Oh, Soo-young, Ryu, Gyu Ha, Jeon, Hong Bae, Chang, Jong Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017458/
https://www.ncbi.nlm.nih.gov/pubmed/35450345
http://dx.doi.org/10.1155/2022/4711499
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author Kim, Sun Jeong
Park, Sang Eon
Jeong, Jang Bin
Oh, Shin Ji
Choi, Alee
Kim, Yun Hee
Choi, Suk-joo
Oh, Soo-young
Ryu, Gyu Ha
Jeon, Hong Bae
Chang, Jong Wook
author_facet Kim, Sun Jeong
Park, Sang Eon
Jeong, Jang Bin
Oh, Shin Ji
Choi, Alee
Kim, Yun Hee
Choi, Suk-joo
Oh, Soo-young
Ryu, Gyu Ha
Jeon, Hong Bae
Chang, Jong Wook
author_sort Kim, Sun Jeong
collection PubMed
description Mesenchymal stem cells (MSCs) are effective therapeutic agents that contribute to tissue repair and regeneration by secreting various factors. However, donor-dependent variations in MSC proliferation and therapeutic potentials result in variable production yields and clinical outcomes, thereby impeding MSC-based therapies. Hence, selection of MSCs with high proliferation and therapeutic potentials would be important for effective clinical application of MSCs. This study is aimed at identifying the upregulated genes in human Wharton's jelly-derived MSCs (WJ-MSCs) with high proliferation potential using mRNA sequencing. Aurora kinase A (AURKA) and dedicator of cytokinesis 2 (DOCK2) were selected as the upregulated genes, and their effects on proliferation, migration, and colony formation of the WJ-MSCs were verified using small interfering RNA (siRNA) techniques. mRNA expression levels of both the genes were positively correlated with the proliferation capacity of WJ-MSCs. Moreover, AURKA from human WJ-MSCs regulated the antiapoptotic effect of skeletal muscle cells by upregulating the chemokine (C motif) ligand (XCL1); this was further confirmed in the mdx mouse model. Taken together, the results indicated that AURKA and DOCK2 can be used as potential biomarkers for proliferation and migration of human WJ-MSCs. In particular, human WJ-MSCs with high expression of AURKA might have therapeutic efficacy against muscle diseases, such as Duchenne muscular dystrophy (DMD).
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spelling pubmed-90174582022-04-20 Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential Kim, Sun Jeong Park, Sang Eon Jeong, Jang Bin Oh, Shin Ji Choi, Alee Kim, Yun Hee Choi, Suk-joo Oh, Soo-young Ryu, Gyu Ha Jeon, Hong Bae Chang, Jong Wook Stem Cells Int Research Article Mesenchymal stem cells (MSCs) are effective therapeutic agents that contribute to tissue repair and regeneration by secreting various factors. However, donor-dependent variations in MSC proliferation and therapeutic potentials result in variable production yields and clinical outcomes, thereby impeding MSC-based therapies. Hence, selection of MSCs with high proliferation and therapeutic potentials would be important for effective clinical application of MSCs. This study is aimed at identifying the upregulated genes in human Wharton's jelly-derived MSCs (WJ-MSCs) with high proliferation potential using mRNA sequencing. Aurora kinase A (AURKA) and dedicator of cytokinesis 2 (DOCK2) were selected as the upregulated genes, and their effects on proliferation, migration, and colony formation of the WJ-MSCs were verified using small interfering RNA (siRNA) techniques. mRNA expression levels of both the genes were positively correlated with the proliferation capacity of WJ-MSCs. Moreover, AURKA from human WJ-MSCs regulated the antiapoptotic effect of skeletal muscle cells by upregulating the chemokine (C motif) ligand (XCL1); this was further confirmed in the mdx mouse model. Taken together, the results indicated that AURKA and DOCK2 can be used as potential biomarkers for proliferation and migration of human WJ-MSCs. In particular, human WJ-MSCs with high expression of AURKA might have therapeutic efficacy against muscle diseases, such as Duchenne muscular dystrophy (DMD). Hindawi 2022-04-11 /pmc/articles/PMC9017458/ /pubmed/35450345 http://dx.doi.org/10.1155/2022/4711499 Text en Copyright © 2022 Sun Jeong Kim et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Sun Jeong
Park, Sang Eon
Jeong, Jang Bin
Oh, Shin Ji
Choi, Alee
Kim, Yun Hee
Choi, Suk-joo
Oh, Soo-young
Ryu, Gyu Ha
Jeon, Hong Bae
Chang, Jong Wook
Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential
title Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential
title_full Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential
title_fullStr Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential
title_full_unstemmed Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential
title_short Wharton's Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential
title_sort wharton's jelly-derived mesenchymal stem cells with high aurora kinase a expression show improved proliferation, migration, and therapeutic potential
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017458/
https://www.ncbi.nlm.nih.gov/pubmed/35450345
http://dx.doi.org/10.1155/2022/4711499
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