Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells

BACKGROUND: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells. METHODS: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also appl...

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Detalles Bibliográficos
Autores principales: Simonian, Miganoosh, Haji Ghaffari, Mozhan, Salimi, Ali, Mirzadegan, Ebrahim, Sadeghi, Niloufar, Ebrahimnezhad, Nasim, Fazli, Ghazaleh, Fatemi, Ramina, Bayat, Ali-Ahmad, Milani, Saeideh, Negahdari, Babak, Rabbani, Hodjattallah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017463/
https://www.ncbi.nlm.nih.gov/pubmed/35509360
http://dx.doi.org/10.18502/ajmb.v14i1.8168
Descripción
Sumario:BACKGROUND: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells. METHODS: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines. RESULTS: Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines. CONCLUSION: Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.

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