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Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis

OBJECTIVES: To screen the potential epigenetic biomarkers associated with endometriosis (EMS) and traditional Chinese medicine (TCM) syndrome EMS types. METHODS: A cohort of 99 participants comprising 42 EMS patients with cold coagulation blood stasis (CCBS) syndrome, 35 EMS patients with Qi stagnat...

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Autores principales: Wang, Xinyue, Zhang, Zhaofeng, Zeng, Weiwei, Zhong, Yuqing, Xie, Dandan, Zhu, Weiqiang, Chen, Fujia, Du, Jing, Zhang, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017494/
https://www.ncbi.nlm.nih.gov/pubmed/35449808
http://dx.doi.org/10.1155/2022/4866360
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author Wang, Xinyue
Zhang, Zhaofeng
Zeng, Weiwei
Zhong, Yuqing
Xie, Dandan
Zhu, Weiqiang
Chen, Fujia
Du, Jing
Zhang, Tingting
author_facet Wang, Xinyue
Zhang, Zhaofeng
Zeng, Weiwei
Zhong, Yuqing
Xie, Dandan
Zhu, Weiqiang
Chen, Fujia
Du, Jing
Zhang, Tingting
author_sort Wang, Xinyue
collection PubMed
description OBJECTIVES: To screen the potential epigenetic biomarkers associated with endometriosis (EMS) and traditional Chinese medicine (TCM) syndrome EMS types. METHODS: A cohort of 99 participants comprising 42 EMS patients with cold coagulation blood stasis (CCBS) syndrome, 35 EMS patients with Qi stagnation blood stasis (QSBS) syndrome, and 22 women of childbearing age without EMS were recruited. Reduced representation bisulfite sequencing (RRBS) was used to establish the differential DNA methylation profiles in human peripheral blood samples obtained from four non-EMS and four EMS patients with CCBS or QSBS syndrome, respectively. Differentially expressed genes (DEGs) were verified in 18 non-EMS, 38 CCBS-EMS, and 31 QSBS-EMS using pyrosequencing. RESULTS: Methylation sites of 123942, 127229, and 115961 were found in peripheral blood DNA of non-EMS, CCBS-EMS, and QSBS-EMS patients, respectively. GO and KEGG analyses showed that the pathological process of EMS may be closely related to the nervous system development, cell junctions, GABA-gated chloride ion channel activity, nicotine addiction, Hippo signaling pathway, mRNA surveillance pathway, and Wnt signaling pathway. The methylation level at CpG site within HDAC6 gene in QSBS-EMS patients was significantly different from that in control women. CONCLUSIONS: The changes in DNA methylation in peripheral blood samples may be associated with EMS and TCM syndrome EMS types. The methylation level of HDAC6 gene may be used to distinguish QSBS-EMS patients from women without EMS.
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spelling pubmed-90174942022-04-20 Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis Wang, Xinyue Zhang, Zhaofeng Zeng, Weiwei Zhong, Yuqing Xie, Dandan Zhu, Weiqiang Chen, Fujia Du, Jing Zhang, Tingting Evid Based Complement Alternat Med Research Article OBJECTIVES: To screen the potential epigenetic biomarkers associated with endometriosis (EMS) and traditional Chinese medicine (TCM) syndrome EMS types. METHODS: A cohort of 99 participants comprising 42 EMS patients with cold coagulation blood stasis (CCBS) syndrome, 35 EMS patients with Qi stagnation blood stasis (QSBS) syndrome, and 22 women of childbearing age without EMS were recruited. Reduced representation bisulfite sequencing (RRBS) was used to establish the differential DNA methylation profiles in human peripheral blood samples obtained from four non-EMS and four EMS patients with CCBS or QSBS syndrome, respectively. Differentially expressed genes (DEGs) were verified in 18 non-EMS, 38 CCBS-EMS, and 31 QSBS-EMS using pyrosequencing. RESULTS: Methylation sites of 123942, 127229, and 115961 were found in peripheral blood DNA of non-EMS, CCBS-EMS, and QSBS-EMS patients, respectively. GO and KEGG analyses showed that the pathological process of EMS may be closely related to the nervous system development, cell junctions, GABA-gated chloride ion channel activity, nicotine addiction, Hippo signaling pathway, mRNA surveillance pathway, and Wnt signaling pathway. The methylation level at CpG site within HDAC6 gene in QSBS-EMS patients was significantly different from that in control women. CONCLUSIONS: The changes in DNA methylation in peripheral blood samples may be associated with EMS and TCM syndrome EMS types. The methylation level of HDAC6 gene may be used to distinguish QSBS-EMS patients from women without EMS. Hindawi 2022-04-11 /pmc/articles/PMC9017494/ /pubmed/35449808 http://dx.doi.org/10.1155/2022/4866360 Text en Copyright © 2022 Xinyue Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Xinyue
Zhang, Zhaofeng
Zeng, Weiwei
Zhong, Yuqing
Xie, Dandan
Zhu, Weiqiang
Chen, Fujia
Du, Jing
Zhang, Tingting
Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis
title Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis
title_full Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis
title_fullStr Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis
title_full_unstemmed Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis
title_short Establishment of DNA Methylation Profile Associated with TCM Syndrome in Endometriosis
title_sort establishment of dna methylation profile associated with tcm syndrome in endometriosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017494/
https://www.ncbi.nlm.nih.gov/pubmed/35449808
http://dx.doi.org/10.1155/2022/4866360
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