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Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics

Drug-induced liver injury (DILI) results in over 100 000 hospital attendances per year in the UK alone and is a leading cause for the post-marketing withdrawal of new drugs, leading to significant financial losses. MicroRNA-122 (miR-122) has been proposed as a sensitive DILI marker although no comme...

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Autores principales: Kersaudy-Kerhoas, Maïwenn, Liga, Antonio, Roychoudhury, Appan, Stamouli, Marilena, Grant, Rhiannon, Carrera, Damaso Sanchez, Schulze, Holger, Mielczarek, Witold, Oosthuyzen, Wilna, Quintana, Juan F., Dickinson, Paul, Buck, Amy H., Leslie, Nicholas R., Haas, Jurgen, Bachmann, Till T., Dear, James W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018095/
https://www.ncbi.nlm.nih.gov/pubmed/35464137
http://dx.doi.org/10.1063/5.0085078
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author Kersaudy-Kerhoas, Maïwenn
Liga, Antonio
Roychoudhury, Appan
Stamouli, Marilena
Grant, Rhiannon
Carrera, Damaso Sanchez
Schulze, Holger
Mielczarek, Witold
Oosthuyzen, Wilna
Quintana, Juan F.
Dickinson, Paul
Buck, Amy H.
Leslie, Nicholas R.
Haas, Jurgen
Bachmann, Till T.
Dear, James W.
author_facet Kersaudy-Kerhoas, Maïwenn
Liga, Antonio
Roychoudhury, Appan
Stamouli, Marilena
Grant, Rhiannon
Carrera, Damaso Sanchez
Schulze, Holger
Mielczarek, Witold
Oosthuyzen, Wilna
Quintana, Juan F.
Dickinson, Paul
Buck, Amy H.
Leslie, Nicholas R.
Haas, Jurgen
Bachmann, Till T.
Dear, James W.
author_sort Kersaudy-Kerhoas, Maïwenn
collection PubMed
description Drug-induced liver injury (DILI) results in over 100 000 hospital attendances per year in the UK alone and is a leading cause for the post-marketing withdrawal of new drugs, leading to significant financial losses. MicroRNA-122 (miR-122) has been proposed as a sensitive DILI marker although no commercial applications are available yet. Extracellular blood microRNAs (miRNAs) are promising clinical biomarkers but their measurement at point of care remains time-consuming, technically challenging, and expensive. For circulating miRNA to have an impact on healthcare, a key challenge to overcome is the development of rapid and reliable low-cost sample preparation. There is an acknowledged issue with miRNA stability in the presence of hemolysis and platelet activation, and no solution has been demonstrated for fast and robust extraction at the site of blood draw. Here, we report a novel microfluidic platform for the extraction of circulating miR-122 from blood enabled by a vertical approach and gravity-based bubble mixing. The performance of this disposable cartridge was verified by standard quantitative polymerase chain reaction analysis on extracted miR-122. The cartridge performed equivalently or better than standard bench extraction kits. The extraction cartridge was combined with electrochemical impedance spectroscopy to detect miR-122 as an initial proof-of-concept toward an application in point-of-care detection. This platform enables the standardization of sample preparation and the detection of miRNAs at the point of blood draw and in resource limited settings and could aid the introduction of miRNA-based assays into routine clinical practice.
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spelling pubmed-90180952022-04-22 Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics Kersaudy-Kerhoas, Maïwenn Liga, Antonio Roychoudhury, Appan Stamouli, Marilena Grant, Rhiannon Carrera, Damaso Sanchez Schulze, Holger Mielczarek, Witold Oosthuyzen, Wilna Quintana, Juan F. Dickinson, Paul Buck, Amy H. Leslie, Nicholas R. Haas, Jurgen Bachmann, Till T. Dear, James W. Biomicrofluidics Regular Articles Drug-induced liver injury (DILI) results in over 100 000 hospital attendances per year in the UK alone and is a leading cause for the post-marketing withdrawal of new drugs, leading to significant financial losses. MicroRNA-122 (miR-122) has been proposed as a sensitive DILI marker although no commercial applications are available yet. Extracellular blood microRNAs (miRNAs) are promising clinical biomarkers but their measurement at point of care remains time-consuming, technically challenging, and expensive. For circulating miRNA to have an impact on healthcare, a key challenge to overcome is the development of rapid and reliable low-cost sample preparation. There is an acknowledged issue with miRNA stability in the presence of hemolysis and platelet activation, and no solution has been demonstrated for fast and robust extraction at the site of blood draw. Here, we report a novel microfluidic platform for the extraction of circulating miR-122 from blood enabled by a vertical approach and gravity-based bubble mixing. The performance of this disposable cartridge was verified by standard quantitative polymerase chain reaction analysis on extracted miR-122. The cartridge performed equivalently or better than standard bench extraction kits. The extraction cartridge was combined with electrochemical impedance spectroscopy to detect miR-122 as an initial proof-of-concept toward an application in point-of-care detection. This platform enables the standardization of sample preparation and the detection of miRNAs at the point of blood draw and in resource limited settings and could aid the introduction of miRNA-based assays into routine clinical practice. AIP Publishing LLC 2022-04-18 /pmc/articles/PMC9018095/ /pubmed/35464137 http://dx.doi.org/10.1063/5.0085078 Text en © 2022 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). Published open access through an agreement withJISC Collections128554
spellingShingle Regular Articles
Kersaudy-Kerhoas, Maïwenn
Liga, Antonio
Roychoudhury, Appan
Stamouli, Marilena
Grant, Rhiannon
Carrera, Damaso Sanchez
Schulze, Holger
Mielczarek, Witold
Oosthuyzen, Wilna
Quintana, Juan F.
Dickinson, Paul
Buck, Amy H.
Leslie, Nicholas R.
Haas, Jurgen
Bachmann, Till T.
Dear, James W.
Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics
title Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics
title_full Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics
title_fullStr Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics
title_full_unstemmed Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics
title_short Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics
title_sort microfluidic system for near-patient extraction and detection of mir-122 microrna biomarker for drug-induced liver injury diagnostics
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018095/
https://www.ncbi.nlm.nih.gov/pubmed/35464137
http://dx.doi.org/10.1063/5.0085078
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