A protocol to assess cellular bioenergetics in flavivirus-infected cells

Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propagation, t...

Descripción completa

Detalles Bibliográficos
Autores principales: Low, John Z.H., Yau, Clement, Zhang, Summer L.X., Tan, Hwee Cheng, Ooi, Eng Eong, Chan, Kuan Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018450/
https://www.ncbi.nlm.nih.gov/pubmed/35463466
http://dx.doi.org/10.1016/j.xpro.2022.101297
Descripción
Sumario:Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propagation, titering and infection, cell preparation, and procedures for mitochondrial and glycolytic stress tests. The protocol enables assessment of cellular respiration and glycolytic flux in flavivirus-infected cells. For complete details on the use and execution of this protocol, please refer to Yau et al. (2021).

Ejemplares similares