Detection of muscle stem cell-derived myonuclei in murine overloaded muscles

Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for...

Descripción completa

Detalles Bibliográficos
Autores principales: Kaneshige, Akihiro, Kaji, Takayuki, Saito, Hayato, Higashimoto, Tatsuyoshi, Nakamura, Ayasa, Kurosawa, Tamaki, Ikemoto-Uezumi, Madoka, Uezumi, Akiyoshi, Fukada, So-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018451/
https://www.ncbi.nlm.nih.gov/pubmed/35463471
http://dx.doi.org/10.1016/j.xpro.2022.101307
_version_ 1784689052706930688
author Kaneshige, Akihiro
Kaji, Takayuki
Saito, Hayato
Higashimoto, Tatsuyoshi
Nakamura, Ayasa
Kurosawa, Tamaki
Ikemoto-Uezumi, Madoka
Uezumi, Akiyoshi
Fukada, So-ichiro
author_facet Kaneshige, Akihiro
Kaji, Takayuki
Saito, Hayato
Higashimoto, Tatsuyoshi
Nakamura, Ayasa
Kurosawa, Tamaki
Ikemoto-Uezumi, Madoka
Uezumi, Akiyoshi
Fukada, So-ichiro
author_sort Kaneshige, Akihiro
collection PubMed
description Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation. We then describe immunostaining for EdU(+) myonuclei and image acquisition for quantitative analyses. For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022).
format Online
Article
Text
id pubmed-9018451
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-90184512022-04-21 Detection of muscle stem cell-derived myonuclei in murine overloaded muscles Kaneshige, Akihiro Kaji, Takayuki Saito, Hayato Higashimoto, Tatsuyoshi Nakamura, Ayasa Kurosawa, Tamaki Ikemoto-Uezumi, Madoka Uezumi, Akiyoshi Fukada, So-ichiro STAR Protoc Protocol Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation. We then describe immunostaining for EdU(+) myonuclei and image acquisition for quantitative analyses. For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022). Elsevier 2022-04-11 /pmc/articles/PMC9018451/ /pubmed/35463471 http://dx.doi.org/10.1016/j.xpro.2022.101307 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Kaneshige, Akihiro
Kaji, Takayuki
Saito, Hayato
Higashimoto, Tatsuyoshi
Nakamura, Ayasa
Kurosawa, Tamaki
Ikemoto-Uezumi, Madoka
Uezumi, Akiyoshi
Fukada, So-ichiro
Detection of muscle stem cell-derived myonuclei in murine overloaded muscles
title Detection of muscle stem cell-derived myonuclei in murine overloaded muscles
title_full Detection of muscle stem cell-derived myonuclei in murine overloaded muscles
title_fullStr Detection of muscle stem cell-derived myonuclei in murine overloaded muscles
title_full_unstemmed Detection of muscle stem cell-derived myonuclei in murine overloaded muscles
title_short Detection of muscle stem cell-derived myonuclei in murine overloaded muscles
title_sort detection of muscle stem cell-derived myonuclei in murine overloaded muscles
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018451/
https://www.ncbi.nlm.nih.gov/pubmed/35463471
http://dx.doi.org/10.1016/j.xpro.2022.101307
work_keys_str_mv AT kaneshigeakihiro detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT kajitakayuki detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT saitohayato detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT higashimototatsuyoshi detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT nakamuraayasa detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT kurosawatamaki detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT ikemotouezumimadoka detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT uezumiakiyoshi detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles
AT fukadasoichiro detectionofmusclestemcellderivedmyonucleiinmurineoverloadedmuscles

Ejemplares similares