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Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics

The gut microbes play important roles in human longevity and the gut microbiota profile of centenarians shows some unique features from young adults. Nowadays, most microbial studies on longevity are commonly based on metagenomic sequencing which may lose information about the functional microbes wi...

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Autores principales: Li, Congyong, Luan, Zhe, Zhao, Yiming, Chen, Jun, Yang, Yanan, Wang, Cong, Jing, Yujia, Qi, Shirui, Li, Zhuanyu, Guo, Hao, Xu, Wenyi, Zhao, Bowen, Wu, Chongming, Wang, Shufang, Yang, Yunsheng, Sun, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019030/
https://www.ncbi.nlm.nih.gov/pubmed/35440640
http://dx.doi.org/10.1038/s41522-022-00282-3
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author Li, Congyong
Luan, Zhe
Zhao, Yiming
Chen, Jun
Yang, Yanan
Wang, Cong
Jing, Yujia
Qi, Shirui
Li, Zhuanyu
Guo, Hao
Xu, Wenyi
Zhao, Bowen
Wu, Chongming
Wang, Shufang
Yang, Yunsheng
Sun, Gang
author_facet Li, Congyong
Luan, Zhe
Zhao, Yiming
Chen, Jun
Yang, Yanan
Wang, Cong
Jing, Yujia
Qi, Shirui
Li, Zhuanyu
Guo, Hao
Xu, Wenyi
Zhao, Bowen
Wu, Chongming
Wang, Shufang
Yang, Yunsheng
Sun, Gang
author_sort Li, Congyong
collection PubMed
description The gut microbes play important roles in human longevity and the gut microbiota profile of centenarians shows some unique features from young adults. Nowadays, most microbial studies on longevity are commonly based on metagenomic sequencing which may lose information about the functional microbes with extremely low abundance. Here, we combined in-depth metagenomic sequencing and large-scale culturomics to reveal the unique gut microbial structure of a Chinese longevity population, and to explore the possible relationship between intestinal microbes and longevity. Twenty-five healthy Hainan natives were enrolled in the study, including 12 centenarians and 13 senior neighbors. An average of 51.1 Gb raw sequencing data were obtained from individual fecal sample. We assembled 1778 non-redundant metagenomic assembled genomes (MAGs), 33.46% of which cannot be classified into known species. Comparison with the ordinary people in Hainan province, the longevous cohort displayed significantly decreased abundance of butyrate-producing bacteria and largely increased proportion of Escherichia coli, Desulfovibrio piger and Methanobrevibacter smithii. These species showed a constant change with aging. We also isolated 8,030 strains from these samples by large-scale culturomics, most of which belonged to 203 known species as identified by MALDI-TOF. Surprisingly, only 42.17% of the isolated species were also detected by metagenomics, indicating obvious complementarity between these two approaches. Combination of two complement methods, in-depth metagenomic sequencing and culturomics, provides deeper insights into the longevity-related gut microbiota. The uniquely enriched gut microbes in Hainan extreme decades population may help to promote health and longevity.
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spelling pubmed-90190302022-04-28 Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics Li, Congyong Luan, Zhe Zhao, Yiming Chen, Jun Yang, Yanan Wang, Cong Jing, Yujia Qi, Shirui Li, Zhuanyu Guo, Hao Xu, Wenyi Zhao, Bowen Wu, Chongming Wang, Shufang Yang, Yunsheng Sun, Gang NPJ Biofilms Microbiomes Article The gut microbes play important roles in human longevity and the gut microbiota profile of centenarians shows some unique features from young adults. Nowadays, most microbial studies on longevity are commonly based on metagenomic sequencing which may lose information about the functional microbes with extremely low abundance. Here, we combined in-depth metagenomic sequencing and large-scale culturomics to reveal the unique gut microbial structure of a Chinese longevity population, and to explore the possible relationship between intestinal microbes and longevity. Twenty-five healthy Hainan natives were enrolled in the study, including 12 centenarians and 13 senior neighbors. An average of 51.1 Gb raw sequencing data were obtained from individual fecal sample. We assembled 1778 non-redundant metagenomic assembled genomes (MAGs), 33.46% of which cannot be classified into known species. Comparison with the ordinary people in Hainan province, the longevous cohort displayed significantly decreased abundance of butyrate-producing bacteria and largely increased proportion of Escherichia coli, Desulfovibrio piger and Methanobrevibacter smithii. These species showed a constant change with aging. We also isolated 8,030 strains from these samples by large-scale culturomics, most of which belonged to 203 known species as identified by MALDI-TOF. Surprisingly, only 42.17% of the isolated species were also detected by metagenomics, indicating obvious complementarity between these two approaches. Combination of two complement methods, in-depth metagenomic sequencing and culturomics, provides deeper insights into the longevity-related gut microbiota. The uniquely enriched gut microbes in Hainan extreme decades population may help to promote health and longevity. Nature Publishing Group UK 2022-04-19 /pmc/articles/PMC9019030/ /pubmed/35440640 http://dx.doi.org/10.1038/s41522-022-00282-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Li, Congyong
Luan, Zhe
Zhao, Yiming
Chen, Jun
Yang, Yanan
Wang, Cong
Jing, Yujia
Qi, Shirui
Li, Zhuanyu
Guo, Hao
Xu, Wenyi
Zhao, Bowen
Wu, Chongming
Wang, Shufang
Yang, Yunsheng
Sun, Gang
Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics
title Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics
title_full Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics
title_fullStr Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics
title_full_unstemmed Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics
title_short Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics
title_sort deep insights into the gut microbial community of extreme longevity in south chinese centenarians by ultra-deep metagenomics and large-scale culturomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019030/
https://www.ncbi.nlm.nih.gov/pubmed/35440640
http://dx.doi.org/10.1038/s41522-022-00282-3
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