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Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities

Cyanidin-3-glucoside (C3G) and peonidin-3-glucoside (P3G) in black rice grain (BRG) demonstrate many beneficial health effects, including antioxidant and anti-aging properties. This research aimed to study on pulsed electric field assisted water extraction (PEF-AWE) on BRG using pre-treatment techni...

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Autores principales: Salee, Nuttinee, Chaiyana, Wantida, Yawootti, Artit, Naruenartwongsakul, Srisuwan, Klangpetch, Wannaporn, Walter, Ponjan, Utama-ang, Niramon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019085/
https://www.ncbi.nlm.nih.gov/pubmed/35440745
http://dx.doi.org/10.1038/s41598-022-10272-2
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author Salee, Nuttinee
Chaiyana, Wantida
Yawootti, Artit
Naruenartwongsakul, Srisuwan
Klangpetch, Wannaporn
Walter, Ponjan
Utama-ang, Niramon
author_facet Salee, Nuttinee
Chaiyana, Wantida
Yawootti, Artit
Naruenartwongsakul, Srisuwan
Klangpetch, Wannaporn
Walter, Ponjan
Utama-ang, Niramon
author_sort Salee, Nuttinee
collection PubMed
description Cyanidin-3-glucoside (C3G) and peonidin-3-glucoside (P3G) in black rice grain (BRG) demonstrate many beneficial health effects, including antioxidant and anti-aging properties. This research aimed to study on pulsed electric field assisted water extraction (PEF-AWE) on BRG using pre-treatment technique, which was determined for enhanced yields of C3G and P3G, antioxidant and sirtuin1 enzyme stimulation activities. The effects of operating parameters for PEF-AWE (intensity of electric field, X(1): 3–5 kV/cm, number of pulse, X(2): 1000–3000 pulse and BRG/water ratio, X(3): 0.1–0.5 g/mL) were determined using regression analysis and optimized PEF-AWE condition using the response surface methodology. Regression models showed the intensity of electric field and BRG/water ratio were the strong influence parameters significantly on C3G (p < 0.01). The results highlighted optimized conditions of PEF-AWE followed by 5 kV/cm, 3000 pulse and 0.5 g/mL leading to achieve higher C3G (92.59 ± 4.79 mg/g) and P3G (4.59 ± 0.27 mg/g) than no pre-treatment by PEF process, approximately 60%. Additionally, PEF extracts of BRG can modulate the ability of surtuin1 enzyme to deacetylate substrate proteins (26.78 ± 0.50 FIR). Thus, PEF-AWE can be applied to produce BRG extract as natural antioxidant compound and functional ingredient.
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spelling pubmed-90190852022-04-21 Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities Salee, Nuttinee Chaiyana, Wantida Yawootti, Artit Naruenartwongsakul, Srisuwan Klangpetch, Wannaporn Walter, Ponjan Utama-ang, Niramon Sci Rep Article Cyanidin-3-glucoside (C3G) and peonidin-3-glucoside (P3G) in black rice grain (BRG) demonstrate many beneficial health effects, including antioxidant and anti-aging properties. This research aimed to study on pulsed electric field assisted water extraction (PEF-AWE) on BRG using pre-treatment technique, which was determined for enhanced yields of C3G and P3G, antioxidant and sirtuin1 enzyme stimulation activities. The effects of operating parameters for PEF-AWE (intensity of electric field, X(1): 3–5 kV/cm, number of pulse, X(2): 1000–3000 pulse and BRG/water ratio, X(3): 0.1–0.5 g/mL) were determined using regression analysis and optimized PEF-AWE condition using the response surface methodology. Regression models showed the intensity of electric field and BRG/water ratio were the strong influence parameters significantly on C3G (p < 0.01). The results highlighted optimized conditions of PEF-AWE followed by 5 kV/cm, 3000 pulse and 0.5 g/mL leading to achieve higher C3G (92.59 ± 4.79 mg/g) and P3G (4.59 ± 0.27 mg/g) than no pre-treatment by PEF process, approximately 60%. Additionally, PEF extracts of BRG can modulate the ability of surtuin1 enzyme to deacetylate substrate proteins (26.78 ± 0.50 FIR). Thus, PEF-AWE can be applied to produce BRG extract as natural antioxidant compound and functional ingredient. Nature Publishing Group UK 2022-04-19 /pmc/articles/PMC9019085/ /pubmed/35440745 http://dx.doi.org/10.1038/s41598-022-10272-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Salee, Nuttinee
Chaiyana, Wantida
Yawootti, Artit
Naruenartwongsakul, Srisuwan
Klangpetch, Wannaporn
Walter, Ponjan
Utama-ang, Niramon
Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities
title Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities
title_full Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities
title_fullStr Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities
title_full_unstemmed Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities
title_short Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities
title_sort optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019085/
https://www.ncbi.nlm.nih.gov/pubmed/35440745
http://dx.doi.org/10.1038/s41598-022-10272-2
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