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The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew)

Nesfatin-1 is an anorectic peptide expressed in both peripheral tissues and brain areas involved in the regulation of feeding, emotion and emesis. The aim of the present study is to characterize the distribution of NUCB2/nesfatin-1 in Suncus murinus and to investigate the actions of nesfatin-1 to af...

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Autores principales: Lu, Zengbing, Cui, Dexuan, Liu, Julia Yuen Hang, Jiang, Bin, Ngan, Man Piu, Sakata, Ichiro, Takemi, Shota, Sakai, Takafumi, Lin, Ge, Chan, Sze Wa, Rudd, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019301/
https://www.ncbi.nlm.nih.gov/pubmed/35462894
http://dx.doi.org/10.3389/fphar.2022.858522
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author Lu, Zengbing
Cui, Dexuan
Liu, Julia Yuen Hang
Jiang, Bin
Ngan, Man Piu
Sakata, Ichiro
Takemi, Shota
Sakai, Takafumi
Lin, Ge
Chan, Sze Wa
Rudd, John A.
author_facet Lu, Zengbing
Cui, Dexuan
Liu, Julia Yuen Hang
Jiang, Bin
Ngan, Man Piu
Sakata, Ichiro
Takemi, Shota
Sakai, Takafumi
Lin, Ge
Chan, Sze Wa
Rudd, John A.
author_sort Lu, Zengbing
collection PubMed
description Nesfatin-1 is an anorectic peptide expressed in both peripheral tissues and brain areas involved in the regulation of feeding, emotion and emesis. The aim of the present study is to characterize the distribution of NUCB2/nesfatin-1 in Suncus murinus and to investigate the actions of nesfatin-1 to affect gastrointestinal contractility, emesis, food and water intake, and locomotor activity. The deduced amino acid sequence of S. murinus nesfatin-1 using in silico cloning showed high homology with humans and rodents. NUCB2 mRNA was detected throughout the entire brain and in the gastrointestinal tract, including the stomach and gut. Western blot analysis and immunohistochemistry confirmed the expression of nesfatin-1 protein in these regions. The NUCB2 mRNA levels in the hypothalamus, hippocampus and brainstem were significantly decreased, whereas that in the striatum were increased after 24 h starvation compared to ad libitum-fed animals (p < 0.05). In in vitro studies, nesfatin-1 (0.3–1,000 pM) failed to contract or relax the isolated gastric antrum and intestinal segments. In conscious, freely moving animals, intracerebroventricular administration of nesfatin-1 (1–50 pmol) induced emesis (p < 0.05) and suppressed 6-h cumulative food intake (p < 0.05), without affecting the latency to feeding. Nesfatin-1 (25 pmol, i.c.v.) decreased 24-h cumulative food and water intake by 28.3 and 35.4%, respectively (p < 0.01). No significant differences in locomotor activity were observed. In conclusion, NUCB2/nesfatin-1 might be a potent regulator of feeding and emesis in S. murinus. Further studies are required to elucidate the mechanism of actions of this peptide as a mediator linking the brainstem NUCB2/nesfatin-1 to forebrain system.
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spelling pubmed-90193012022-04-21 The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew) Lu, Zengbing Cui, Dexuan Liu, Julia Yuen Hang Jiang, Bin Ngan, Man Piu Sakata, Ichiro Takemi, Shota Sakai, Takafumi Lin, Ge Chan, Sze Wa Rudd, John A. Front Pharmacol Pharmacology Nesfatin-1 is an anorectic peptide expressed in both peripheral tissues and brain areas involved in the regulation of feeding, emotion and emesis. The aim of the present study is to characterize the distribution of NUCB2/nesfatin-1 in Suncus murinus and to investigate the actions of nesfatin-1 to affect gastrointestinal contractility, emesis, food and water intake, and locomotor activity. The deduced amino acid sequence of S. murinus nesfatin-1 using in silico cloning showed high homology with humans and rodents. NUCB2 mRNA was detected throughout the entire brain and in the gastrointestinal tract, including the stomach and gut. Western blot analysis and immunohistochemistry confirmed the expression of nesfatin-1 protein in these regions. The NUCB2 mRNA levels in the hypothalamus, hippocampus and brainstem were significantly decreased, whereas that in the striatum were increased after 24 h starvation compared to ad libitum-fed animals (p < 0.05). In in vitro studies, nesfatin-1 (0.3–1,000 pM) failed to contract or relax the isolated gastric antrum and intestinal segments. In conscious, freely moving animals, intracerebroventricular administration of nesfatin-1 (1–50 pmol) induced emesis (p < 0.05) and suppressed 6-h cumulative food intake (p < 0.05), without affecting the latency to feeding. Nesfatin-1 (25 pmol, i.c.v.) decreased 24-h cumulative food and water intake by 28.3 and 35.4%, respectively (p < 0.01). No significant differences in locomotor activity were observed. In conclusion, NUCB2/nesfatin-1 might be a potent regulator of feeding and emesis in S. murinus. Further studies are required to elucidate the mechanism of actions of this peptide as a mediator linking the brainstem NUCB2/nesfatin-1 to forebrain system. Frontiers Media S.A. 2022-04-06 /pmc/articles/PMC9019301/ /pubmed/35462894 http://dx.doi.org/10.3389/fphar.2022.858522 Text en Copyright © 2022 Lu, Cui, Liu, Jiang, Ngan, Sakata, Takemi, Sakai, Lin, Chan and Rudd. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Lu, Zengbing
Cui, Dexuan
Liu, Julia Yuen Hang
Jiang, Bin
Ngan, Man Piu
Sakata, Ichiro
Takemi, Shota
Sakai, Takafumi
Lin, Ge
Chan, Sze Wa
Rudd, John A.
The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew)
title The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew)
title_full The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew)
title_fullStr The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew)
title_full_unstemmed The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew)
title_short The Actions of Centrally Administered Nesfatin-1 on Emesis, Feeding, and Locomotor Activity in Suncus murinus (House Musk Shrew)
title_sort actions of centrally administered nesfatin-1 on emesis, feeding, and locomotor activity in suncus murinus (house musk shrew)
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019301/
https://www.ncbi.nlm.nih.gov/pubmed/35462894
http://dx.doi.org/10.3389/fphar.2022.858522
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