Cargando…

Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance

AIMS: To explore the effect of IR on the metabolism of PCOS by analyzing the changes in FF metabolites in PCOS patients who are undergoing assisted reproductive technology based on the metabonomic platform of ultraperformance gas chromatography coupled to mass spectrometry (GC/MS). METHOD: Eight PCO...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Ran, Bai, Shun, Zheng, Shengxia, Zhu, Xinyi, Zhang, Yan, Xu, Bo, Zhao, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019454/
https://www.ncbi.nlm.nih.gov/pubmed/35465261
http://dx.doi.org/10.1155/2022/6877541
_version_ 1784689283265724416
author Liu, Ran
Bai, Shun
Zheng, Shengxia
Zhu, Xinyi
Zhang, Yan
Xu, Bo
Zhao, Weidong
author_facet Liu, Ran
Bai, Shun
Zheng, Shengxia
Zhu, Xinyi
Zhang, Yan
Xu, Bo
Zhao, Weidong
author_sort Liu, Ran
collection PubMed
description AIMS: To explore the effect of IR on the metabolism of PCOS by analyzing the changes in FF metabolites in PCOS patients who are undergoing assisted reproductive technology based on the metabonomic platform of ultraperformance gas chromatography coupled to mass spectrometry (GC/MS). METHOD: Eight PCOS patients with IR (PCOS-IR) and 8 PCOS patients without IR (PCOS-NIR) were enrolled. All patients received controlled ovarian stimulation by using the gonadotropin-releasing hormone (GnRH) antagonist protocol, and the FF of a single dominant follicle was collected on the day of oocyte retrieval. The metabolite profiles of the FF were determined by GC/MS. Key Results. A total of 20 differentially expressed metabolites in FF were identified. Compared with levels in the PCOS-NIR group, stearic acid, palmitic acid, pentadecanoic acid, stigmasterol, citric acid, isocitric acid, thymine, and pyruvic acid in FF were significantly increased in the PCOS-IR group. Lithocholic acid and sinapinic acid in FF decreased significantly. The affected metabolic pathways with potential regulatory roles were identified by KEGG annotation. CONCLUSION: Compared with the PCOS-NIR group, the PCOS-IR group showed more significant metabolic abnormalities. Implications. These results will help us to understand the pathogenesis of PCOS combined with IR and will provide new clues for studying metabolic disorders associated with PCOS, e.g., IR.
format Online
Article
Text
id pubmed-9019454
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-90194542022-04-21 Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance Liu, Ran Bai, Shun Zheng, Shengxia Zhu, Xinyi Zhang, Yan Xu, Bo Zhao, Weidong Dis Markers Research Article AIMS: To explore the effect of IR on the metabolism of PCOS by analyzing the changes in FF metabolites in PCOS patients who are undergoing assisted reproductive technology based on the metabonomic platform of ultraperformance gas chromatography coupled to mass spectrometry (GC/MS). METHOD: Eight PCOS patients with IR (PCOS-IR) and 8 PCOS patients without IR (PCOS-NIR) were enrolled. All patients received controlled ovarian stimulation by using the gonadotropin-releasing hormone (GnRH) antagonist protocol, and the FF of a single dominant follicle was collected on the day of oocyte retrieval. The metabolite profiles of the FF were determined by GC/MS. Key Results. A total of 20 differentially expressed metabolites in FF were identified. Compared with levels in the PCOS-NIR group, stearic acid, palmitic acid, pentadecanoic acid, stigmasterol, citric acid, isocitric acid, thymine, and pyruvic acid in FF were significantly increased in the PCOS-IR group. Lithocholic acid and sinapinic acid in FF decreased significantly. The affected metabolic pathways with potential regulatory roles were identified by KEGG annotation. CONCLUSION: Compared with the PCOS-NIR group, the PCOS-IR group showed more significant metabolic abnormalities. Implications. These results will help us to understand the pathogenesis of PCOS combined with IR and will provide new clues for studying metabolic disorders associated with PCOS, e.g., IR. Hindawi 2022-03-20 /pmc/articles/PMC9019454/ /pubmed/35465261 http://dx.doi.org/10.1155/2022/6877541 Text en Copyright © 2022 Ran Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Ran
Bai, Shun
Zheng, Shengxia
Zhu, Xinyi
Zhang, Yan
Xu, Bo
Zhao, Weidong
Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance
title Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance
title_full Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance
title_fullStr Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance
title_full_unstemmed Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance
title_short Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance
title_sort identification of the metabolomics signature of human follicular fluid from pcos women with insulin resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019454/
https://www.ncbi.nlm.nih.gov/pubmed/35465261
http://dx.doi.org/10.1155/2022/6877541
work_keys_str_mv AT liuran identificationofthemetabolomicssignatureofhumanfollicularfluidfrompcoswomenwithinsulinresistance
AT baishun identificationofthemetabolomicssignatureofhumanfollicularfluidfrompcoswomenwithinsulinresistance
AT zhengshengxia identificationofthemetabolomicssignatureofhumanfollicularfluidfrompcoswomenwithinsulinresistance
AT zhuxinyi identificationofthemetabolomicssignatureofhumanfollicularfluidfrompcoswomenwithinsulinresistance
AT zhangyan identificationofthemetabolomicssignatureofhumanfollicularfluidfrompcoswomenwithinsulinresistance
AT xubo identificationofthemetabolomicssignatureofhumanfollicularfluidfrompcoswomenwithinsulinresistance
AT zhaoweidong identificationofthemetabolomicssignatureofhumanfollicularfluidfrompcoswomenwithinsulinresistance