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Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance
AIMS: To explore the effect of IR on the metabolism of PCOS by analyzing the changes in FF metabolites in PCOS patients who are undergoing assisted reproductive technology based on the metabonomic platform of ultraperformance gas chromatography coupled to mass spectrometry (GC/MS). METHOD: Eight PCO...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019454/ https://www.ncbi.nlm.nih.gov/pubmed/35465261 http://dx.doi.org/10.1155/2022/6877541 |
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author | Liu, Ran Bai, Shun Zheng, Shengxia Zhu, Xinyi Zhang, Yan Xu, Bo Zhao, Weidong |
author_facet | Liu, Ran Bai, Shun Zheng, Shengxia Zhu, Xinyi Zhang, Yan Xu, Bo Zhao, Weidong |
author_sort | Liu, Ran |
collection | PubMed |
description | AIMS: To explore the effect of IR on the metabolism of PCOS by analyzing the changes in FF metabolites in PCOS patients who are undergoing assisted reproductive technology based on the metabonomic platform of ultraperformance gas chromatography coupled to mass spectrometry (GC/MS). METHOD: Eight PCOS patients with IR (PCOS-IR) and 8 PCOS patients without IR (PCOS-NIR) were enrolled. All patients received controlled ovarian stimulation by using the gonadotropin-releasing hormone (GnRH) antagonist protocol, and the FF of a single dominant follicle was collected on the day of oocyte retrieval. The metabolite profiles of the FF were determined by GC/MS. Key Results. A total of 20 differentially expressed metabolites in FF were identified. Compared with levels in the PCOS-NIR group, stearic acid, palmitic acid, pentadecanoic acid, stigmasterol, citric acid, isocitric acid, thymine, and pyruvic acid in FF were significantly increased in the PCOS-IR group. Lithocholic acid and sinapinic acid in FF decreased significantly. The affected metabolic pathways with potential regulatory roles were identified by KEGG annotation. CONCLUSION: Compared with the PCOS-NIR group, the PCOS-IR group showed more significant metabolic abnormalities. Implications. These results will help us to understand the pathogenesis of PCOS combined with IR and will provide new clues for studying metabolic disorders associated with PCOS, e.g., IR. |
format | Online Article Text |
id | pubmed-9019454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-90194542022-04-21 Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance Liu, Ran Bai, Shun Zheng, Shengxia Zhu, Xinyi Zhang, Yan Xu, Bo Zhao, Weidong Dis Markers Research Article AIMS: To explore the effect of IR on the metabolism of PCOS by analyzing the changes in FF metabolites in PCOS patients who are undergoing assisted reproductive technology based on the metabonomic platform of ultraperformance gas chromatography coupled to mass spectrometry (GC/MS). METHOD: Eight PCOS patients with IR (PCOS-IR) and 8 PCOS patients without IR (PCOS-NIR) were enrolled. All patients received controlled ovarian stimulation by using the gonadotropin-releasing hormone (GnRH) antagonist protocol, and the FF of a single dominant follicle was collected on the day of oocyte retrieval. The metabolite profiles of the FF were determined by GC/MS. Key Results. A total of 20 differentially expressed metabolites in FF were identified. Compared with levels in the PCOS-NIR group, stearic acid, palmitic acid, pentadecanoic acid, stigmasterol, citric acid, isocitric acid, thymine, and pyruvic acid in FF were significantly increased in the PCOS-IR group. Lithocholic acid and sinapinic acid in FF decreased significantly. The affected metabolic pathways with potential regulatory roles were identified by KEGG annotation. CONCLUSION: Compared with the PCOS-NIR group, the PCOS-IR group showed more significant metabolic abnormalities. Implications. These results will help us to understand the pathogenesis of PCOS combined with IR and will provide new clues for studying metabolic disorders associated with PCOS, e.g., IR. Hindawi 2022-03-20 /pmc/articles/PMC9019454/ /pubmed/35465261 http://dx.doi.org/10.1155/2022/6877541 Text en Copyright © 2022 Ran Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Ran Bai, Shun Zheng, Shengxia Zhu, Xinyi Zhang, Yan Xu, Bo Zhao, Weidong Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance |
title | Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance |
title_full | Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance |
title_fullStr | Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance |
title_full_unstemmed | Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance |
title_short | Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance |
title_sort | identification of the metabolomics signature of human follicular fluid from pcos women with insulin resistance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019454/ https://www.ncbi.nlm.nih.gov/pubmed/35465261 http://dx.doi.org/10.1155/2022/6877541 |
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