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Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota

Bifidobacteria is an important microbe that inhabits the human gut. It is capable of metabolizing complex compounds in the human diet. Albiflorin, an antidepressant natural product from Radix Paeoniae Alba in China, is difficult to absorb after oral administration, and its metabolism has been proven...

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Autores principales: Peng, Ran, Han, Pei, Fu, Jie, Zhang, Zheng-Wei, Ma, Shu-Rong, Pan, Li-Bin, Xia, Yuan-Yuan, Yu, Hang, Xu, Hui, Liu, Chang-Xiao, Wang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019491/
https://www.ncbi.nlm.nih.gov/pubmed/35464989
http://dx.doi.org/10.3389/fmicb.2022.880118
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author Peng, Ran
Han, Pei
Fu, Jie
Zhang, Zheng-Wei
Ma, Shu-Rong
Pan, Li-Bin
Xia, Yuan-Yuan
Yu, Hang
Xu, Hui
Liu, Chang-Xiao
Wang, Yan
author_facet Peng, Ran
Han, Pei
Fu, Jie
Zhang, Zheng-Wei
Ma, Shu-Rong
Pan, Li-Bin
Xia, Yuan-Yuan
Yu, Hang
Xu, Hui
Liu, Chang-Xiao
Wang, Yan
author_sort Peng, Ran
collection PubMed
description Bifidobacteria is an important microbe that inhabits the human gut. It is capable of metabolizing complex compounds in the human diet. Albiflorin, an antidepressant natural product from Radix Paeoniae Alba in China, is difficult to absorb after oral administration, and its metabolism has been proven to be closely related to the gut microbiota. In this study, we demonstrated in vitro that several Bifidobacteria species were able to convert albiflorin to benzoic acid, and four esterases (B2, B3, B4, and BL) from Bifidobacterium breve and Bifidobacterium longum were found through genome mining and modeled by SWISS-MODEL. B2 and B3 presented the strongest albiflorin metabolism ability. The optimal conditions, including temperature, buffer, and pH, for the conversion of albiflorin by the four esterases were investigated. Furthermore, the effect of esterase on the metabolism of albiflorin in vivo was confirmed by transplanting bacteria containing esterase B2. This study demonstrated the vital role of esterases from Bifidobacteria in the metabolism of natural compounds containing ester bonds, which could contribute to the development of new enzymes, microbial evolution, and probiotic adjuvant compounds for treatment.
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spelling pubmed-90194912022-04-21 Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota Peng, Ran Han, Pei Fu, Jie Zhang, Zheng-Wei Ma, Shu-Rong Pan, Li-Bin Xia, Yuan-Yuan Yu, Hang Xu, Hui Liu, Chang-Xiao Wang, Yan Front Microbiol Microbiology Bifidobacteria is an important microbe that inhabits the human gut. It is capable of metabolizing complex compounds in the human diet. Albiflorin, an antidepressant natural product from Radix Paeoniae Alba in China, is difficult to absorb after oral administration, and its metabolism has been proven to be closely related to the gut microbiota. In this study, we demonstrated in vitro that several Bifidobacteria species were able to convert albiflorin to benzoic acid, and four esterases (B2, B3, B4, and BL) from Bifidobacterium breve and Bifidobacterium longum were found through genome mining and modeled by SWISS-MODEL. B2 and B3 presented the strongest albiflorin metabolism ability. The optimal conditions, including temperature, buffer, and pH, for the conversion of albiflorin by the four esterases were investigated. Furthermore, the effect of esterase on the metabolism of albiflorin in vivo was confirmed by transplanting bacteria containing esterase B2. This study demonstrated the vital role of esterases from Bifidobacteria in the metabolism of natural compounds containing ester bonds, which could contribute to the development of new enzymes, microbial evolution, and probiotic adjuvant compounds for treatment. Frontiers Media S.A. 2022-04-06 /pmc/articles/PMC9019491/ /pubmed/35464989 http://dx.doi.org/10.3389/fmicb.2022.880118 Text en Copyright © 2022 Peng, Han, Fu, Zhang, Ma, Pan, Xia, Yu, Xu, Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Peng, Ran
Han, Pei
Fu, Jie
Zhang, Zheng-Wei
Ma, Shu-Rong
Pan, Li-Bin
Xia, Yuan-Yuan
Yu, Hang
Xu, Hui
Liu, Chang-Xiao
Wang, Yan
Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota
title Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota
title_full Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota
title_fullStr Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota
title_full_unstemmed Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota
title_short Esterases From Bifidobacteria Exhibit the Conversion of Albiflorin in Gut Microbiota
title_sort esterases from bifidobacteria exhibit the conversion of albiflorin in gut microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019491/
https://www.ncbi.nlm.nih.gov/pubmed/35464989
http://dx.doi.org/10.3389/fmicb.2022.880118
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