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Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia
Circular RNA (circRNA) is an endogenous biomolecule in eukaryotes. It has tissue- and cell-specific expression patterns and can act as a microRNA sponge or competitive endogenous RNA. Although circRNA has been found in several species in recent years, the expression profiles in fish gonad are still...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019548/ https://www.ncbi.nlm.nih.gov/pubmed/35464370 http://dx.doi.org/10.3389/fvets.2022.847681 |
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author | Zhong, Huan Guo, Zhongbao Xiao, Jun Zhang, Hong Luo, Yongju Liang, Junneng |
author_facet | Zhong, Huan Guo, Zhongbao Xiao, Jun Zhang, Hong Luo, Yongju Liang, Junneng |
author_sort | Zhong, Huan |
collection | PubMed |
description | Circular RNA (circRNA) is an endogenous biomolecule in eukaryotes. It has tissue- and cell-specific expression patterns and can act as a microRNA sponge or competitive endogenous RNA. Although circRNA has been found in several species in recent years, the expression profiles in fish gonad are still not fully understood. We detected the expression of circRNA in the ovary, testis, and sex-changed gonad of tilapia by high-throughput deep sequencing, and circRNA-specific computing tools. A total of 20,607 circRNAs were obtained, of which 141 were differentially expressed in the testis and ovary. Among these circRNAs, 135 circRNAs were upregulated and 6 circRNAs were downregulated in female fish. In addition, GO annotation and KEGG pathway analysis of the host genes of circRNAs indicated that these host genes were mainly involved in adherens junction, androgen production, and reproductive development, such as ZP3, PLC, delta 4a, ARHGEF10, and HSD17b3. It is worth noting that we found that circRNAs in tilapia gonads have abundant miRNA-binding sites. Among them, 935 circRNAs have a regulatory effect on miR-212, 856 circRNAs have a regulatory effect on miR-200b-3p, and 529 circRNAs have a regulatory effect on miR-200b-5p. Thus, our findings provide a new evidence for circRNA–miRNA networks in the gonads in tilapia. |
format | Online Article Text |
id | pubmed-9019548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90195482022-04-21 Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia Zhong, Huan Guo, Zhongbao Xiao, Jun Zhang, Hong Luo, Yongju Liang, Junneng Front Vet Sci Veterinary Science Circular RNA (circRNA) is an endogenous biomolecule in eukaryotes. It has tissue- and cell-specific expression patterns and can act as a microRNA sponge or competitive endogenous RNA. Although circRNA has been found in several species in recent years, the expression profiles in fish gonad are still not fully understood. We detected the expression of circRNA in the ovary, testis, and sex-changed gonad of tilapia by high-throughput deep sequencing, and circRNA-specific computing tools. A total of 20,607 circRNAs were obtained, of which 141 were differentially expressed in the testis and ovary. Among these circRNAs, 135 circRNAs were upregulated and 6 circRNAs were downregulated in female fish. In addition, GO annotation and KEGG pathway analysis of the host genes of circRNAs indicated that these host genes were mainly involved in adherens junction, androgen production, and reproductive development, such as ZP3, PLC, delta 4a, ARHGEF10, and HSD17b3. It is worth noting that we found that circRNAs in tilapia gonads have abundant miRNA-binding sites. Among them, 935 circRNAs have a regulatory effect on miR-212, 856 circRNAs have a regulatory effect on miR-200b-3p, and 529 circRNAs have a regulatory effect on miR-200b-5p. Thus, our findings provide a new evidence for circRNA–miRNA networks in the gonads in tilapia. Frontiers Media S.A. 2022-04-06 /pmc/articles/PMC9019548/ /pubmed/35464370 http://dx.doi.org/10.3389/fvets.2022.847681 Text en Copyright © 2022 Zhong, Guo, Xiao, Zhang, Luo and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Zhong, Huan Guo, Zhongbao Xiao, Jun Zhang, Hong Luo, Yongju Liang, Junneng Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia |
title | Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia |
title_full | Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia |
title_fullStr | Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia |
title_full_unstemmed | Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia |
title_short | Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia |
title_sort | comprehensive characterization of circular rnas in ovary and testis from nile tilapia |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019548/ https://www.ncbi.nlm.nih.gov/pubmed/35464370 http://dx.doi.org/10.3389/fvets.2022.847681 |
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